Switch to Europe/Worldwide website Switch to German website Switch to French website Switch to OriGene Technologies, Inc. (North America)
Home » Target » GRAP2 antibody

GRAP2 antibody

(reset)

Principal name

GRAP2 antibody

Alternative names for GRAP2 antibody

GADS, GRBLG, GRB2L, Grf-40, GRBX, GRID, GRB2-related adapter protein 2, Growth factor receptor-binding protein, GRB-2-like protein, Grf40 adapter protein, Hematopoietic cell-associated adapter protein GrpL, Adapter protein GRID, SH3-SH2-SH3 adapter Mona

SwissProt ID

O75791 (Human), O89100 (Mouse)

Gene ID

9402 (GRAP2), 17444 (Grap2)

Ncbi ID

9606, NP_004801, NP_004801.1, NP_034945.1, NM_010815

Available reactivities

Hu (Human), Ms (Mouse), Por (Porcine)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunoprecipitation (IP), Flow Cytometry (F)

Background of GRAP2 antibody

In general, it exists in association with catalytic domains, as in the nonreceptor protein-tyrosine kinases and phospholipase C-γ, within structural proteins, such as spectrin or myosin, and in small adapter proteins, such as Crk and GRB2. SH3 domains are often accompanied by SH2 domains of 100 amino acids that bind to tyrosine-phosphorylated regions of target proteins, frequently linking activated growth factors to putative signal transduction proteins. Deletion or mutation of SH3 domains generally activates the transforming potential of nonreceptor tyrosine kinases, suggesting that SH3 mediates negative regulation of an intrinsic transforming activity. Gads is an adapter proteins that contains both SH2 and SH3 domains. Gads binds to tyrosine-phosphorylated proteins, such as Shc, and functions to couple these proteins to downstream effectors.

General readings

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010)
Preisinger, C., et al. Cell. Signal. 22(5):848-856 (2010)
Davila, S., et al. Genes Immun. 11(3):232-238 (2010)
Segat, L., et al. J. Gastroenterol. Hepatol. 24(12):1840-1846 (2009)
Ludwig, L., et al. Cancer Lett. 275(2):194-197 (2009)

H00009402-M01 - GRAP2
Immunoperoxidase of monoclonal antibody to GRAP2 on formalin-fixed paraffin-embedded human spleen. [antibody concentration 3 ug/ml]
H00009402-M01 - GRAP2
Western Blot analysis of GRAP2 expression in transfected 293T cell line by GRAP2 monoclonal antibody (M01), clone 1G12.Lane 1: GRAP2 transfected lysate(38 KDa).Lane 2: Non-transfected lysate.
H00009402-M01 - GRAP2
GRAP2 monoclonal antibody (M01), clone 1G12 Western Blot analysis of GRAP2 expression in Jurkat ( Cat # L017V1 ).
H00009402-M01 - GRAP2
Detection limit for recombinant GST tagged GRAP2 is approximately 0.03ng/ml as a capture antibody.
H00009402-B02 - GRAP2
Western Blot analysis of GRAP2 expression in transfected 293T cell line (H00009402-T01) by GRAP2 MaxPab polyclonal antibody.Lane 1: GRAP2 transfected lysate(36.3 KDa).Lane 2: Non-transfected lysate.
H00009402-B01 - GRAP2
Western Blot analysis of GRAP2 expression in transfected 293T cell line (H00009402-T02) by GRAP2 MaxPab polyclonal antibody.Lane 1: GRAP2 transfected lysate(36.3 KDa).Lane 2: Non-transfected lysate.
PAB6036 - GRAP2
HEK293 overexpressing GRAP2 and probed with GRAP2 polyclonal antibody ( Cat # PAB6036 ) ( mock transfection in first lane ), tested by Origene.
PAB6036 - GRAP2
GRAP2 polyclonal antibody ( Cat # PAB6036 ) staining ( 0.5 µg/ml ) of Jurkat lysate ( RIPA buffer, 30 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
H00009402-D01P - GRAP2
Proximity Ligation Analysis (PLA) of protein-protein interactions between GRAP2 and CSF1R. HeLa cells were stained with anti-GRAP2 rabbit purified polyclonal 1:1200 and anti-CSF1R mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
H00009402-D01P - GRAP2
Western Blot analysis of GRAP2 expression in transfected 293T cell line (H00009402-T03) by GRAP2 MaxPab polyclonal antibody.Lane 1: GRAP2 transfected lysate(37.90 KDa).Lane 2: Non-transfected lysate.
H00009402-D01P - GRAP2
GRAP2 MaxPab rabbit polyclonal antibody. Western Blot analysis of GRAP2 expression in Jurkat.
H00009402-D01 - GRAP2
GRAP2 MaxPab rabbit polyclonal antibody. Western Blot analysis of GRAP2 expression in Jurkat.
H00009402-D01 - GRAP2
Western Blot analysis of GRAP2 expression in transfected 293T cell line (H00009402-T03) by GRAP2 MaxPab polyclonal antibody.Lane 1: GRAP2 transfected lysate(37.90 KDa).Lane 2: Non-transfected lysate.
H00009402-D01 - GRAP2
Immunoprecipitation of GRAP2 transfected lysate using anti-GRAP2 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with GRAP2 MaxPab mouse polyclonal antibody (B02) (H00009402-B02).
AP21183PU-N - GRAP2
Western blot (WB) analysis of Gads antibody (Cat.-No.: AP21183PU-N) in extracts from HUVEC cells.
C15966-1 - GRAP2
Western blot analysis of extracts from HuvEc cells, using GRAP2 Antibody. The lane on the right is treated with the synthesized peptide.
AR09828PU-L - GRAP2
AR09828PU-L - GRAP2
Recombinant human GRAP2, 1-330 aa, His-tagged
AP51943PU-N - GRAP2
Western blot analysis of GRAP2 Antibody (Center) Cat.-No AP51943PU-N in MCF-7 cell line lysates (35ug/lane). This demonstrates the GRAP2 antibody detected the GRAP2 protein (arrow).
TA502923 - GRAP2
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-GRAP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502923)
TA502923 - GRAP2
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-GRAP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502923)
TA502923 - GRAP2
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-GRAP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502923)
TA502923 - GRAP2
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRAP2 (RC206546, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRAP2.
TA502923 - GRAP2
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GRAP2 monoclonal antibody.
TA502923 - GRAP2
HEK293T cells transfected with either RC206546 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-GRAP2 antibody(TA502923), and then analyzed by flow cytometry.
TA502923 - GRAP2
Flow cytometric Analysis of Jurkat cells, using anti-GRAP2 antibody(TA502923),(Red), compared to a nonspecific negative control antibody,(Blue).
TP306546 - GRAP2
TA302501 - GRAP2
TA302501 staining (0.5µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
TA308365 - GRAP2
Sample (30 ug of whole cell lysate). A: JurKat. 10% SDS PAGE. TA308365 diluted at 1:1000.
TA308365 - GRAP2
Immunohistochemical analysis of paraffin-embedded Colon ca, using GRAP2(TA308365) antibody at 1:500 dilution.
TA312173 - GRAP2
Western blot analysis of extracts from HuvEc cells, using GRAP2 antibody.The lane on the right is treated with the synthesized peptide.
TA346954 - GRAP2
Western blot detection of GRAP2 in GRAP2 positive cell (CEM,Molt-4,Jurkat) and negative cell (3T3,K562,Hela) lysates using GRAP2 mouse mAb (1:1000 diluted).Predicted band size:38KDa.Observed band size:38KDa.

1 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GRAP2 (C-term) antibody
  Goat Polyclonal Antibody against GRAP2 / GRID / Grf40  
TA302501 staining (0.5µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Aff - Purified Hu, Ms, Por WB
0.1 mg / €360.00
  OriGene Technologies, Inc.

1 Item(s)

per page

Active Filters

  1. Remove This Filter Reactivity: Porcine (Por)
Clear All Filters

Available Filters

Product Type
  1. Primary Antibody (1)
Clonality
  1. Polyclonal (1)
Target
  1. GRAP2 (1)
Property
  1. C-term (1)
Applications
  1. Western blot / Immunoblot (WB) (1)
Presentation
  1. Aff - Purified (1)
Host
  1. Goat (1)
Images
  1. 1 image (1)
Images: Applications
  1. Western blot / Immunoblot (WB) (1)
Manufacturer
  1. OriGene Technologies (1)
Service
  • OriGene Technologies GmbH
  • Schillerstraße 5
  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
  • email: info-de@origene.com
  • orders: ordering-de@origene.com
 
© Copyright 1998 - 2018 OriGene Technologies GmbH
  • LinkedIn