GTF2B antibody
Principal name
GTF2B antibody
Alternative names for GTF2B antibody
Transcription initiation factor IIB, TF2B, TFIIB, General transcription factor TFIIB, S300-II
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Immunoprecipitation (IP)
Background of GTF2B antibody
GTF2B is the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II.This gene encodes the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
General readings
Tubon,T.C., et al., (2004) Mol. Cell. Biol. 24 (7), 2863-2874
Human Intestine; GTF2B antibody - N-terminal region (P100990_T100) in Human Intestine cells using Immunohistochemistry
Human HepG2; WB Suggested Anti-GTF2B Antibody Titration: 4.0ug/ml. Positive Control: HepG2 cell lysate; GTF2B antibody - N-terminal region (P100990_T100) in Human HepG2 cells using Western Blot
Immunofluorescence of purified MaxPab antibody to GTF2B on HeLa cell. [antibody concentration 10 ug/ml]
GTF2B MaxPab polyclonal antibody. Western Blot analysis of GTF2B expression in human pancreas.
Western Blot analysis of GTF2B expression in transfected 293T cell line (H00002959-T01) by GTF2B MaxPab polyclonal antibody.Lane 1: GTF2B transfected lysate(34.87 KDa).Lane 2: Non-transfected lysate.
Immunofluorescence of purified MaxPab antibody to GTF2B on HeLa cell. [antibody concentration 10 ug/ml]
GTF2B MaxPab polyclonal antibody. Western Blot analysis of GTF2B expression in human pancreas.
Western Blot analysis of GTF2B expression in transfected 293T cell line (H00002959-T01) by GTF2B MaxPab polyclonal antibody.Lane 1: GTF2B transfected lysate(34.87 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of GTF2B expression in transfected 293T cell line (H00002959-T02) by GTF2B MaxPab polyclonal antibody.Lane 1: GTF2B transfected lysate(34.76 KDa).Lane 2: Non-transfected lysate.
GTF2B MaxPab polyclonal antibody. Western Blot analysis of GTF2B expression in NIH/3T3.
GTF2B MaxPab polyclonal antibody. Western Blot analysis of GTF2B expression in Raw 264.7.
GTF2B MaxPab polyclonal antibody. Western Blot analysis of GTF2B expression in Hela NE.
GTF2B MaxPab polyclonal antibody. Western Blot analysis of GTF2B expression in PC-12.
GTF2B MaxPab rabbit polyclonal antibody. Western Blot analysis of GTF2B expression in mouse kidney.
GTF2B MaxPab rabbit polyclonal antibody. Western Blot analysis of GTF2B expression in human kidney.
Western Blot analysis of GTF2B expression in transfected 293T cell line (H00002959-T03) by GTF2B MaxPab polyclonal antibody.Lane 1: GTF2B transfected lysate(34.80 KDa).Lane 2: Non-transfected lysate.
Recombinant human GTF2B (1-316 aa), His-tagged: 15% SDS-PAGE (3 µg)
Human Placenta; WB Suggested Anti-GTF2B Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Placenta; GTF2B antibody - C-terminal region (ARP58006_P050) in Human Placenta cells using Western Blot
Human Brain; WB Suggested Anti-GTF2B Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human brain; GTF2B antibody - N-terminal region (P100989_P050) in Human Brain cells using Western Blot
Rat Lung; WB Suggested Anti-Gtf2b Antibody. . Titration: 1.0 ug/ml. . Positive Control: Rat lung; Gtf2b antibody - N-terminal region (ARP58005_P050) in Rat Lung cells using Western Blot
Western blot analysis using GTF2B mouse mAb against Hela (1), NIH/3T3 (2), COS7 (3) and A431 (4) cell lysate.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GTF2B (RC204607, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GTF2B.(1:2000)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GTF2B (RC204607, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GTF2B.(1:2000)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GTF2B (RC204607, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GTF2B.(1:2000)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GTF2B (RC204607, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GTF2B.(1:2000)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GTF2B (RC204607, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GTF2B.(1:2000)
WB Suggested Anti-GTF2B Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human brain
WB Suggested Anti-GTF2B Antibody Titration: 4.0ug/ml; Positive Control: HepG2 cell lysate
Human Intestine
WB Suggested Anti-GTF2B Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human Placenta
Western blot detection of TFIIB in Jurkat,MCF7,COS7,3T3 and Hela cell lysates using TFIIB mouse mAb (1:500 diluted).Observed band size: 34KDa
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA346956 | GTF2B antibody |
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| Mouse Monoclonal TFIIB Antibody | |||||||
 |
Mouse | IgG2b | 2F6-A3 | Purified | WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA808547 | GTF2B antibody |
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| GTF2B mouse monoclonal antibody, clone OTI1H10 (formerly 1H10) | |||||||
 |
Mouse | IgG1 | OTI1H10 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA808571 | GTF2B antibody |
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| GTF2B mouse monoclonal antibody, clone OTI2A7 (formerly 2A7) | |||||||
 |
Mouse | IgG1 | OTI2A7 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA808572 | GTF2B antibody |
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| GTF2B mouse monoclonal antibody, clone OTI2A1 (formerly 2A1) | |||||||
 |
Mouse | IgG1 | OTI2A1 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA808573 | GTF2B antibody |
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| GTF2B mouse monoclonal antibody, clone OTI2F5 (formerly 2F5) | |||||||
 |
Mouse | IgG1 | OTI2F5 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA808602 | GTF2B antibody |
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| GTF2B mouse monoclonal antibody, clone OTI5B6 (formerly 5B6) | |||||||
 |
Mouse | IgG2b | OTI5B6 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| AM06454SU-N | GTF2B antibody |
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 |
Mouse | IgG1 | 4C1 | Ascites | Hu, Mky | E, WB |
0.1 ml /
€380.00
|
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP304607 | GTF2B |
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| Recombinant protein of human general transcription factor IIB (GTF2B) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP720263 | GTF2B |
||||
| Recombinant protein of human general transcription factor IIB (GTF2B) | |||||
 |
Human | > 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
10 µg /
€299.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY400582 | GTF2B overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR204535L2V | Lenti ORF particles, Gtf2b (GFP-tagged) - Mouse general transcription factor IIB (Gtf2b), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR204535L1V | Lenti ORF particles, Gtf2b (Myc-DDK-tagged) - Mouse general transcription factor IIB (Gtf2b), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR207197L2V | Lenti ORF particles, Gtf2b (GFP-tagged ORF) - Rat general transcription factor IIB (Gtf2b), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR207197L1V | Lenti ORF particles, Gtf2b (Myc-DDK-tagged ORF) - Rat general transcription factor IIB (Gtf2b), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
