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GTF2B antibody

Principal name

GTF2B antibody

Alternative names for GTF2B antibody

Transcription initiation factor IIB, TF2B, TFIIB, General transcription factor TFIIB, S300-II

SwissProt ID

P62915 (Mouse), P62916 (Rat), Q00403 (Human), Q2KIN8 (Bovin)

Gene ID

2959 (GTF2B), 81673 (Gtf2b), 229906

Ncbi ID

9606, NP_001505, NM_031041, NP_001505.1, NM_001514, NM_145546

Available reactivities

Bov (Bovine), Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Mky (Monkey), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Mam (Mammalia)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Immunoprecipitation (IP)

Background of GTF2B antibody

GTF2B is the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II.This gene encodes the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Tubon,T.C., et al., (2004) Mol. Cell. Biol. 24 (7), 2863-2874

Human Intestine; GTF2B antibody - N-terminal region (P100990_T100) in Human Intestine cells using Immunohistochemistry

P100990_T100

Human HepG2; WB Suggested Anti-GTF2B Antibody Titration: 4.0ug/ml. Positive Control: HepG2 cell lysate; GTF2B antibody - N-terminal region (P100990_T100) in Human HepG2 cells using Western Blot

P100990_T100

Immunofluorescence of purified MaxPab antibody to GTF2B on HeLa cell. [antibody concentration 10 ug/ml]

H00002959-B01

GTF2B MaxPab polyclonal antibody. Western Blot analysis of GTF2B expression in human pancreas.

H00002959-B01

Western Blot analysis of GTF2B expression in transfected 293T cell line (H00002959-T01) by GTF2B MaxPab polyclonal antibody.Lane 1: GTF2B transfected lysate(34.87 KDa).Lane 2: Non-transfected lysate.

H00002959-B01

Immunofluorescence of purified MaxPab antibody to GTF2B on HeLa cell. [antibody concentration 10 ug/ml]

H00002959-B01P

GTF2B MaxPab polyclonal antibody. Western Blot analysis of GTF2B expression in human pancreas.

H00002959-B01P

Western Blot analysis of GTF2B expression in transfected 293T cell line (H00002959-T01) by GTF2B MaxPab polyclonal antibody.Lane 1: GTF2B transfected lysate(34.87 KDa).Lane 2: Non-transfected lysate.

H00002959-B01P

Western Blot analysis of GTF2B expression in transfected 293T cell line (H00002959-T02) by GTF2B MaxPab polyclonal antibody.Lane 1: GTF2B transfected lysate(34.76 KDa).Lane 2: Non-transfected lysate.

H00002959-B02

GTF2B MaxPab polyclonal antibody. Western Blot analysis of GTF2B expression in NIH/3T3.

H00002959-B02

GTF2B MaxPab polyclonal antibody. Western Blot analysis of GTF2B expression in Raw 264.7.

H00002959-B02

GTF2B MaxPab polyclonal antibody. Western Blot analysis of GTF2B expression in Hela NE.

H00002959-B02

GTF2B MaxPab polyclonal antibody. Western Blot analysis of GTF2B expression in PC-12.

H00002959-B02

GTF2B MaxPab rabbit polyclonal antibody. Western Blot analysis of GTF2B expression in mouse kidney.

H00002959-D01P

GTF2B MaxPab rabbit polyclonal antibody. Western Blot analysis of GTF2B expression in human kidney.

H00002959-D01P

Western Blot analysis of GTF2B expression in transfected 293T cell line (H00002959-T03) by GTF2B MaxPab polyclonal antibody.Lane 1: GTF2B transfected lysate(34.80 KDa).Lane 2: Non-transfected lysate.

H00002959-D01P

Recombinant human GTF2B (1-316 aa), His-tagged: 15% SDS-PAGE (3 µg)

Human Placenta; WB Suggested Anti-GTF2B Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Placenta; GTF2B antibody - C-terminal region (ARP58006_P050) in Human Placenta cells using Western Blot

ARP58006_P050

Human Brain; WB Suggested Anti-GTF2B Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human brain; GTF2B antibody - N-terminal region (P100989_P050) in Human Brain cells using Western Blot

P100989_P050

Rat Lung; WB Suggested Anti-Gtf2b Antibody. . Titration: 1.0 ug/ml. . Positive Control: Rat lung; Gtf2b antibody - N-terminal region (ARP58005_P050) in Rat Lung cells using Western Blot

ARP58005_P050

Western blot analysis using GTF2B mouse mAb against Hela (1), NIH/3T3 (2), COS7 (3) and A431 (4) cell lysate.

AM06454SU-N

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GTF2B (RC204607, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GTF2B.(1:2000)