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IRF4 antibody

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Principal name

IRF4 antibody

Alternative names for IRF4 antibody

Interferon regulatory factor 4, NF-EM5, MUM1, LSIRF, Lymphocyte-specific interferon regulatory factor, Multiple myeloma oncogene 1

SwissProt ID

Q15306 (Human), Q64287 (Mouse)

Gene ID

3662 (IRF4), 16364 (Irf4)

Ncbi ID

NP_001182215.1, 9606, NP_002451, NP_001182215, NP_002451.2, NP_038702.1, NM_013674

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Por (Porcine), Rt (Rat), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Goat, Rat

Available applications

Frozen Sections (C), Paraffin Sections (P), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F)

Background of IRF4 antibody

Kit Component:
- KN308426G1, Irf4 gRNA vector 1 in pCas-Guide vector
- KN308426G2, Irf4 gRNA vector 2 in pCas-Guide vector
- KN308426D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

Ucisik-Akkaya, E., et al. Mol. Hum. Reprod. 16(10):770-777(2010)
Staudt, V., et al. Immunity 33(2):192-202(2010)
Newton-Bishop, J.A., et al. Cancer Epidemiol. Biomarkers Prev. 19(8):2043-2054(2010)
Duffy, D.L., et al. Am. J. Hum. Genet. 87(1):6-16(2010)
Eriksson, N., et al. PLoS Genet. 6 (6), E1000993 (2010)

H00003662-M02 - IRF4
Immunoperoxidase of monoclonal antibody to IRF4 on formalin-fixed paraffin-embedded human colon. [antibody concentration 0.3 ug/ml]
H00003662-M02 - IRF4
Western Blot analysis of IRF4 expression in transfected 293T cell line by IRF4 monoclonal antibody (M02), clone 2F2.Lane 1: IRF4 transfected lysate(51.8 KDa).Lane 2: Non-transfected lysate.
H00003662-M02 - IRF4
Detection limit for recombinant GST tagged IRF4 is approximately 0.03ng/ml as a capture antibody.
H00003662-M04 - IRF4
Western Blot analysis of IRF4 expression in transfected 293T cell line by IRF4 monoclonal antibody (M04), clone 3A10.Lane 1: IRF4 transfected lysate(51.8 KDa).Lane 2: Non-transfected lysate.
H00003662-M04 - IRF4
Detection limit for recombinant GST tagged IRF4 is approximately 0.03ng/ml as a capture antibody.
H00003662-M05 - IRF4
Western Blot analysis of IRF4 expression in transfected 293T cell line by IRF4 monoclonal antibody (M05), clone 2D1.Lane 1: IRF4 transfected lysate(51.8 KDa).Lane 2: Non-transfected lysate.
H00003662-M05 - IRF4
Western blot analysis of IRF4 over-expressed 293 cell line, cotransfected with IRF4 Validated Chimera RNAi ( Cat # H00003662-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with IRF4 monoclonal antibody (M05), clone 2D1 (Cat # H00003662-M05 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
H00003662-M05 - IRF4
Detection limit for recombinant GST tagged IRF4 is approximately 0.03ng/ml as a capture antibody.
H00003662-M10 - IRF4
Western Blot analysis of IRF4 expression in transfected 293T cell line by IRF4 monoclonal antibody (M10), clone 3C4.Lane 1: IRF4 transfected lysate(51.8 KDa).Lane 2: Non-transfected lysate.
ARP32669_T100 - IRF4
Human Jurkat; WB Suggested Anti-IRF4 Antibody Titration: 5ug/ml. Positive Control: Jurkat cell lysate; IRF4 antibody - N-terminal region (ARP32669_T100) in Human Jurkat cells using Western Blot
H00084939-B01P - IRF4
Western Blot analysis of MUM1 expression in transfected 293T cell line (H00084939-T01) by MUM1 MaxPab polyclonal antibody.Lane 1: MUM1 transfected lysate(78.21 KDa).Lane 2: Non-transfected lysate.
AP01237PU-N - IRF4
Western blot (WB) analysis of IRF-4 antibody (Cat.-No. AP01237PU-N) in extracts from HeLa cells.
AP01237PU-N - IRF4
Immunohistochemistry (IHC) analysis of IRF-4 antibody (Cat.-No. AP01237PU-N) in paraffin-embedded human tonsil carcinoma tissue at 1:50.showing nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin peroxidase.
AP01237PU-N - IRF4
Western blot (WB) analysis of IRF-4 antibody (Cat.-No AP01237PU-N) in extracts from A549 cells.
ARP38154_P050 - IRF4
Human Skin; IRF4 antibody - N-terminal region (ARP38154_P050) in Human Skin cells using Immunohistochemistry
ARP38154_P050 - IRF4
Human Jurkat; WB Suggested Anti-IRF4 Antibody Titration: 5.0ug/ml. Positive Control: Jurkat cell lysate; IRF4 antibody - N-terminal region (ARP38154_P050) in Human Jurkat cells using Western Blot
AP15668PU-S - IRF4
AP15668PU IRF4 antibody staining of Human Hodgkin's Lymphoma.
ARP38155_P050 - IRF4
Hum. Fetal Muscle; Host:Rabbit. Target Name:IRF4. Sample Tissue:Human Fetal Muscle. Antibody Dilution: 1.0ug/ml.; IRF4 antibody - middle region (ARP38155_P050) in Hum. Fetal Muscle cells using Western Blot
ARP38155_P050 - IRF4
Human 721_B; WB Suggested Anti-IRF4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 721_B cell lysate. IRF4 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.; IRF4 antibody - middle region (ARP38155_P050) in Human 721_B cells using Western Blot
C11070-1 - IRF4
Western blot analysis of extracts from HeLa/Jurkat cells, using IRF4 Antibody. The lane on the right is treated with the synthesized peptide.
AM20307PU-S - IRF4
Human Hodgkin's lymphoma stained with anti-MUM1/IRF4 antibody.
AM20307PU-S - IRF4
Western Blot analysis of Ramos cell lysate with anti-MUM1/IRF4 antibody
AM20906PU-N - IRF4
Human Neutrophils: Formalin-Fixed, Paraffin-Embedded (FFPE)
AM20906PU-N - IRF4
Human Neutrophils: Formalin-Fixed, Paraffin-Embedded (FFPE)
AM20906PU-N - IRF4
Western Blot analysis of IRF4 expression in transfected 293T cell line by IRF4 monoclonal antibody clone 2F2.
AM20906PU-N - IRF4
Detection limit for recombinant GST tagged IRF4 is approximately 0.03 ng/ml as a capture antibody.
AP52236PU-N - IRF4
Western blot analysis of IRF4 (arrow) using IRF4 Antibody (Center) Cat.-No AP52236PU-N. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the IRF4 gene.
AP52236PU-N - IRF4
Flow cytometric analysis of 293 cells using IRF4 Antibody (Center) Cat.-No AP52236PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
CPA3248-100ul - IRF4
Immunohistochemical analysis of IRF4 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
CPA3248-100ul - IRF4
Immunofluorescent analysis of IRF4 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
CPA3248-100ul - IRF4
Western blot analysis of IRF4 expression in A549 (A); Ramos (B) whole cell lysates.
TP304876 - IRF4
TA311234 - IRF4
(0.5ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA314093 - IRF4
Western blot analysis of extracts from HeLa cells and Jurkat cells, using IRF4 antibody.The lane on the right is treated with the synthesized peptide.
TA319669 - IRF4
Western blot analysis of IRF4 in Jurkat cell lysate with IRF4 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.
TA319669 - IRF4
Immunofluorescence of IRF4 in Jurkat cells with IRF4 antibody at 20 ug/mL.
TA319669 - IRF4
Immunocytochemistry of IRF4 in Jurkat cells with IRF4 antibody at 10 ug/mL.
TA327705 - IRF4
Immunohistochemistry staining of Paraffin Tonsil tissue by MUM1 antibody (dilution: 1:100 - 1:500; visualization of staining: Nuclear, cytoplasmic)
TA327948 - IRF4
Western blot analysis of Daudi (lane 1) , Raji(lane 2) and A20 (lane 3) cells using IRF4 antibody (clone IRF4.3E4). β-actin antibody (poly6221) was used as loading control.
TA331103 - IRF4
WB Suggested Anti-IRF4 Antibody Titration: 5ug/ml; Positive Control: Jurkat cell lysate
TA335827 - IRF4
WB Suggested Anti-IRF4 Antibody Titration: 5.0 ug/ml; Positive Control: Jurkat cell lysate
TA335827 - IRF4
Human Skin
TA335828 - IRF4
Host: Rabbit; Target Name: IRF4; Sample Tissue: Human Fetal Muscle; Antibody Dilution: 1.0 ug/ml
TA335828 - IRF4
WB Suggested Anti-IRF4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: 721_B cell lysate. IRF4 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells
TA347745 - IRF4
Western blot analysis on HeLa cell lysate using IRF4 Antibody
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  2. Highly Validated (1)

14 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

IRF4 antibody
Human Neutrophils: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 2F2 Hu E, P, WB
50 µg / €475.00
  OriGene Technologies GmbH
+3 additional images

Highly validated antibody!

IRF4 (C-term) antibody
  Goat Anti-IRF4 Antibody  
(0.5ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €360.00
  OriGene Technologies, Inc.

IRF4 antibody
  Rabbit polyclonal anti-IRF4 antibody  
Western blot analysis of extracts from HeLa cells and Jurkat cells, using IRF4 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

IRF4 (C-term) antibody
  Rabbit Polyclonal IRF4 Antibody  
Western blot analysis of IRF4 in Jurkat cell lysate with IRF4 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €415.00
  OriGene Technologies, Inc.
+2 additional images

IRF4 antibody
  Rabbit Monoclonal MUM1 Antibody  
Immunohistochemistry staining of Paraffin Tonsil tissue by MUM1 antibody (dilution: 1:100 - 1:500; visualization of staining: Nuclear, cytoplasmic) Rabbit IgG1 MRQ-43 Purified Hu P
1 ml / €720.00
  OriGene Technologies, Inc.

IRF4 (C-term) antibody
  Rat Monoclonal anti-IRF4 Antibody  
Western blot analysis of Daudi (lane 1) , Raji(lane 2) and A20 (lane 3) cells using IRF4 antibody (clone IRF4.3E4). β-actin antibody (poly6221) was used as loading control. Rat IgG1 IRF4.3E4 Purified Hu, Ms F, WB
0.1 mg / €255.00
  OriGene Technologies, Inc.

IRF4 antibody
  Rabbit Polyclonal Anti-IRF4 Antibody  
Western blot analysis on HeLa cell lysate using IRF4 Antibody Rabbit IgG Purified Hu, Ms WB
0.1 mg / €335.00
  OriGene Technologies, Inc.

IRF4 antibody
  Rabbit Polyclonal Anti-IRF4 Antibody  
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Mouse lung tissue, Primary antibody: (IRF4 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

IRF4 antibody
IRF4 Mouse IgG1 KBA.62 Supernatant Hu C, P
1 ml / €760.00
  OriGene Technologies GmbH

IRF4 antibody
Western blot (WB) analysis of IRF-4 antibody (Cat.-No. AP01237PU-N) in extracts from HeLa cells. Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 mg / €370.00
  OriGene Technologies GmbH
+2 additional images

IRF4 (Center) antibody
Western blot analysis of IRF4 (arrow) using IRF4 Antibody (Center) Cat.-No AP52236PU-N. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the IRF4 gene. Rabbit Ig Purified Hu F, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+1 additional image

IRF4 antibody
IRF4 Mouse IgG1 MUM1p Supernatant Hu C, P
1 ml / €1,200.00
  OriGene Technologies GmbH

IRF4 antibody
IRF4 Mouse IgG1 MUM1p Supernatant Hu C, P
0.5 ml / €750.00
  OriGene Technologies GmbH

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

IRF4
  Recombinant protein of human interferon regulatory factor 4 (IRF4)  
IRF4 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

14 Item(s)

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14 products - FILTER YOUR RESULTS!

Available Filters

Product Type
  1. Primary Antibody (13)
  2. Protein | Growth Factor (1)
Clonality
  1. Polyclonal (7)
  2. Monoclonal (6)
Target
  1. IRF4 (14)
Property
  1. C-term (3)
  2. Center (1)
Reactivity
  1. Human (Hu) (13)
  2. Mouse (Ms) (6)
  3. Rat (Rt) (1)
Applications
  1. Western blot / Immunoblot (WB) (9)
  2. Paraffin Sections (P) (6)
  3. Enzyme Immunoassay (E) (5)
  4. Frozen sections (C) (3)
  5. Flow cytometry (F) (2)
  6. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
Presentation
  1. Purified (6)
  2. Aff - Purified (3)
  3. Supernatant (3)
Host
  1. Rabbit (7)
  2. Mouse (4)
  3. Goat (1)
  4. Rat (1)
Images
  1. 1 image (11)
  2. 3+ images (3)
Images: Applications
  1. Western blot / Immunoblot (WB) (9)
  2. Paraffin Sections (P) (2)
  3. ELISA (capture) (E(capture)) (1)
  4. Flow cytometry (F) (1)
  5. Immunofluorescence (IF) (1)
  6. IHC (1)
Species (Protein)
  1. Human (1)
Source
  1. HEK293 cells (1)
Manufacturer
  1. OriGene Technologies (14)
Service
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