IRF4 antibody
Principal name
IRF4 antibody
Alternative names for IRF4 antibody
Interferon regulatory factor 4, NF-EM5, MUM1, LSIRF, Lymphocyte-specific interferon regulatory factor, Multiple myeloma oncogene 1
Ncbi ID
NP_001182215.1, 9606, NP_002451, NP_001182215, NP_002451.2, NP_038702.1, NM_013674
Available reactivities
Available hosts
Available applications
Frozen Sections (C), Paraffin Sections (P), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F)
Background of IRF4 antibody
- Kit Component:
- - KN308426G1, Irf4 gRNA vector 1 in pCas-Guide vector
- KN308426G2, Irf4 gRNA vector 2 in pCas-Guide vector
- KN308426D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Ucisik-Akkaya, E., et al. Mol. Hum. Reprod. 16(10):770-777(2010)
Staudt, V., et al. Immunity 33(2):192-202(2010)
Newton-Bishop, J.A., et al. Cancer Epidemiol. Biomarkers Prev. 19(8):2043-2054(2010)
Duffy, D.L., et al. Am. J. Hum. Genet. 87(1):6-16(2010)
Eriksson, N., et al. PLoS Genet. 6 (6), E1000993 (2010)
Immunoperoxidase of monoclonal antibody to IRF4 on formalin-fixed paraffin-embedded human colon. [antibody concentration 0.3 ug/ml]
Western Blot analysis of IRF4 expression in transfected 293T cell line by IRF4 monoclonal antibody (M02), clone 2F2.Lane 1: IRF4 transfected lysate(51.8 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged IRF4 is approximately 0.03ng/ml as a capture antibody.
Western Blot analysis of IRF4 expression in transfected 293T cell line by IRF4 monoclonal antibody (M04), clone 3A10.Lane 1: IRF4 transfected lysate(51.8 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged IRF4 is approximately 0.03ng/ml as a capture antibody.
Western Blot analysis of IRF4 expression in transfected 293T cell line by IRF4 monoclonal antibody (M05), clone 2D1.Lane 1: IRF4 transfected lysate(51.8 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of IRF4 over-expressed 293 cell line, cotransfected with IRF4 Validated Chimera RNAi ( Cat # H00003662-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with IRF4 monoclonal antibody (M05), clone 2D1 (Cat # H00003662-M05 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Detection limit for recombinant GST tagged IRF4 is approximately 0.03ng/ml as a capture antibody.
Western Blot analysis of IRF4 expression in transfected 293T cell line by IRF4 monoclonal antibody (M10), clone 3C4.Lane 1: IRF4 transfected lysate(51.8 KDa).Lane 2: Non-transfected lysate.
Human Jurkat; WB Suggested Anti-IRF4 Antibody Titration: 5ug/ml. Positive Control: Jurkat cell lysate; IRF4 antibody - N-terminal region (ARP32669_T100) in Human Jurkat cells using Western Blot
Western Blot analysis of MUM1 expression in transfected 293T cell line (H00084939-T01) by MUM1 MaxPab polyclonal antibody.Lane 1: MUM1 transfected lysate(78.21 KDa).Lane 2: Non-transfected lysate.
Western blot (WB) analysis of IRF-4 antibody (Cat.-No. AP01237PU-N) in extracts from HeLa cells.
Immunohistochemistry (IHC) analysis of IRF-4 antibody (Cat.-No. AP01237PU-N) in paraffin-embedded human tonsil carcinoma tissue at 1:50.showing nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin peroxidase.
Western blot (WB) analysis of IRF-4 antibody (Cat.-No AP01237PU-N) in extracts from A549 cells.
Human Skin; IRF4 antibody - N-terminal region (ARP38154_P050) in Human Skin cells using Immunohistochemistry
Human Jurkat; WB Suggested Anti-IRF4 Antibody Titration: 5.0ug/ml. Positive Control: Jurkat cell lysate; IRF4 antibody - N-terminal region (ARP38154_P050) in Human Jurkat cells using Western Blot
AP15668PU IRF4 antibody staining of Human Hodgkin's Lymphoma.
Hum. Fetal Muscle; Host:Rabbit. Target Name:IRF4. Sample Tissue:Human Fetal Muscle. Antibody Dilution: 1.0ug/ml.; IRF4 antibody - middle region (ARP38155_P050) in Hum. Fetal Muscle cells using Western Blot
Human 721_B; WB Suggested Anti-IRF4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 721_B cell lysate. IRF4 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.; IRF4 antibody - middle region (ARP38155_P050) in Human 721_B cells using Western Blot
Human Hodgkin's lymphoma stained with anti-MUM1/IRF4 antibody.
Western Blot analysis of Ramos cell lysate with anti-MUM1/IRF4 antibody
Human Neutrophils: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Neutrophils: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western Blot analysis of IRF4 expression in transfected 293T cell line by IRF4 monoclonal antibody clone 2F2.
Detection limit for recombinant GST tagged IRF4 is approximately 0.03 ng/ml as a capture antibody.
Western blot analysis of IRF4 (arrow) using IRF4 Antibody (Center) Cat.-No AP52236PU-N. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the IRF4 gene.
Flow cytometric analysis of 293 cells using IRF4 Antibody (Center) Cat.-No AP52236PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Immunohistochemical analysis of IRF4 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of IRF4 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of IRF4 expression in A549 (A); Ramos (B) whole cell lysates.
(0.5ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot analysis of extracts from HeLa cells and Jurkat cells, using IRF4 antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of IRF4 in Jurkat cell lysate with IRF4 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.
Immunofluorescence of IRF4 in Jurkat cells with IRF4 antibody at 20 ug/mL.
Immunocytochemistry of IRF4 in Jurkat cells with IRF4 antibody at 10 ug/mL.
Immunohistochemistry staining of Paraffin Tonsil tissue by MUM1 antibody (dilution: 1:100 - 1:500; visualization of staining: Nuclear, cytoplasmic)
Western blot analysis of Daudi (lane 1) , Raji(lane 2) and A20 (lane 3) cells using IRF4 antibody (clone IRF4.3E4). β-actin antibody (poly6221) was used as loading control.
WB Suggested Anti-IRF4 Antibody Titration: 5ug/ml; Positive Control: Jurkat cell lysate
WB Suggested Anti-IRF4 Antibody Titration: 5.0 ug/ml; Positive Control: Jurkat cell lysate
Human Skin
Host: Rabbit; Target Name: IRF4; Sample Tissue: Human Fetal Muscle; Antibody Dilution: 1.0 ug/ml
WB Suggested Anti-IRF4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: 721_B cell lysate. IRF4 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells
Western blot analysis on HeLa cell lysate using IRF4 Antibody
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA311234 | IRF4 (C-term) antibody |
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| Goat Anti-IRF4 Antibody | |||||||
 |
Goat | Aff - Purified | Hu | E, WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA314093 | IRF4 antibody |
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| Rabbit polyclonal anti-IRF4 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA319669 | IRF4 (C-term) antibody |
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| Rabbit Polyclonal IRF4 Antibody | |||||||
 |
Rabbit | Aff - Purified | Hu, Ms, Rt | E, WB |
0.1 mg /
€415.00
|
||
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
|||||||
| TA347745 | IRF4 antibody |
||||||
| Rabbit Polyclonal Anti-IRF4 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | WB |
0.1 mg /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA351302 | IRF4 antibody |
||||||
| Rabbit Polyclonal Anti-IRF4 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP01237PU-N | IRF4 antibody |
||||||
 |
Rabbit | Aff - Purified | Hu, Ms | ICC/IF, P, WB |
0.1 mg /
€370.00
|
||
| OriGene Technologies GmbH | |||||||
| +2 additional images |
|||||||
| AP52236PU-N | IRF4 (Center) antibody |
||||||
 |
Rabbit | Ig | Purified | Hu | F, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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