MSH2 antibody
Principal name
MSH2 antibody
Alternative names for MSH2 antibody
DNA mismatch repair protein Msh2, MutS protein homolog 2
Ncbi ID
NP_000242.1, 9606, AAY15096, NP_000242, NP_000239, NM_000251, NP_032654.1, NM_031058
Available reactivities
Available hosts
Available applications
Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Frozen Sections (C)
Background of MSH2 antibody
Component of the post-replicative D mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to D mismatches thereby initiating D repair. When bound, heterodimers bend the D helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the D. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a terry complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimition, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched D provokes ADP-->ATP exchange, resulting in a discernible conformatiol transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the D backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in D homologous recombition repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.
General readings
Kim, M. et al., Cancer Sci. 101(11): 2436-2442 (2010)
Mangoni, M. et al., Int. J. Radiat. Oncol. Biol. Phys. 81(1): 52-58 (2010)
Srivastava, K. et al., Cancer 116(13): 3160-3169 (2010)
van der Post, R.S. et al., J. Med. Genet. 47(7): 464-470 (2010)
Langner, E.,et al., J. Genet. 89(1): 101-104 (2010)
Proximity Ligation Analysis (PLA) of protein-protein interactions between MAX and MSH2 HeLa cells were stained with anti-MAX rabbit purified polyclonal 1:1200 and anti-MSH2 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
MSH2 polyclonal antibody (A01), Lot # 050727JC01 Western Blot analysis of MSH2 expression in 293 ( Cat # L026V1 ).
Human HepG2; WB Suggested Anti-MSH2 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:62500. Positive Control: HepG2 cell lysate; MSH2 antibody - N-terminal region (ARP41350_T100) in Human HepG2 cells using Western Blot
Immunofluorescence of purified MaxPab antibody to MSH2 on HeLa cell. [antibody concentration 10 ug/ml]
MSH2 MaxPab polyclonal antibody. Western Blot analysis of MSH2 expression in human placenta.
Western Blot analysis of MSH2 expression in transfected 293T cell line (H00004436-T01) by MSH2 MaxPab polyclonal antibody.Lane 1: MSH2 transfected lysate(102.74 KDa).Lane 2: Non-transfected lysate.
Proximity Ligation Analysis (PLA) of protein-protein interactions between MSH2 and MSH6 HeLa cells were stained with anti-MSH2 rabbit purified polyclonal 1:1200 and anti-MSH6 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Western Blot analysis of MSH2 expression in transfected 293T cell line (H00004436-T01) by MSH2 MaxPab polyclonal antibody.Lane 1: MSH2 transfected lysate(104.70 KDa).Lane 2: Non-transfected lysate.
MSH2 MaxPab rabbit polyclonal antibody. Western Blot analysis of MSH2 expression in Hela NE.
Human colon ca stained with Anti-MSH2
Human Colon Carcinoma stained with anti-MSH2 Antibody Cat.-No AM11173PU (Clone SP46).
Cytometric analysis of anti-MSH2 Antibody Cat.-No AM11173PU (Clone SP46) in HeLa (green) compare to negative control of Rabbit IgG (blue)
Breast: Formalin-Fixed Paraffin-Embedded (FFPE)
Immunohistochemical staining using MSH2 monoclonal antibody, clone 2MSH01 ( Cat # MAB1956 ) on formalin fixed, paraffin embedded human tonsil tissue.
Immunohistochemical staining of MSH2 on formalin fixed, paraffin embedded human colon cancer with MSH2 polyclonal antibody.
Immunohistochemical analysis of paraffin-embedded human rectum carcinoma tissue, showing nuclear and cytoplasmic localization using MSH2 monoclonal antibody, clone 3A2B8C ( Cat # MAB5538 ) with DAB staining.
Western blot analysis using MSH2 monoclonal antibody, clone 3A2B8C ( Cat # MAB5538 ) against A-431 cell lysate.
Human kidney; . Sample Type: Human KidneyPrimary. Dilution:. 1ug/mL; MSH2 antibody - N-terminal region (ARP41351_P050) in Human kidney cells using Immunohistochemistry
Human HepG2; WB Suggested Anti-MSH2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate; MSH2 antibody - N-terminal region (ARP41351_P050) in Human HepG2 cells using Western Blot
Formalin-Fixed, Paraffin-Embedded Human colon adenocarcinoma stained with MSH2 antibody Cat.-No. AM10116SU-N using peroxidase conjugate and DAB chromogen. Note the nuclear staining of tumor cells.
Immunohistochemical analysis of paraffin-embedded human breast cancer (left) and lung cancer (right) tissues, showing nuclear localization using MSH2 antibody Cat.-No AM06094SU-N with DAB staining.
Confocal Immunofluorescence analysis of Hela cells using MSH2 antibody Cat.-No AM06094SU-N (green), showing nuclear localization. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Western blot analysis using MSH2 antibody Cat.-No AM06094SU-N against Hela (Lane 1), A549 (Lane 2), A431 (Lane 3) and HEK293 (Lane 4) cell lysate
Immunohistochemistry analysis of paraffin-embedded human brain tissue using MSH2 antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HUVEC cells, using MSH2 antibody.The picture on the right is treated with the synthesized peptide.
MSH2 Antibody (Center) (Cat. #TA324411) western blot analysis in SW480,U251 cell line lysates (35ug/lane).This demonstrates the MSH2 antibody detected the MSH2 protein (arrow).
Confocal immunofluorescent analysis of MSH2 Antibody (Center)(Cat. #TA324411) with Hela cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
MSH2 Antibody (Center) (Cat. #TA324411) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of extracts of various cell lines, using MSH2 antibody.
Immunofluorescence analysis of HeLa cell using MSH2 antibody. Blue: DAPI for nuclear staining.
Immunohistochemistry staining of Paraffin Colon tissue by MSH2 antibody (dilution: 1:100-1:500; visualization of staining: Nuclear)
MSH2 Antibody (Center) (Cat. #TA328162) western blot analysis in 293,Hela cell line lysates (35ug/lane).This demonstrates the MSH2 antibody detected the MSH2 protein (arrow).
WB Suggested Anti-MSH2 Antibody Titration: 1.25 ug/ml; ELISA Titer: 1: 62500; Positive Control: HepG2 cell lysate
WB Suggested Anti-MSH2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: HepG2 cell lysate
Sample Type: Human KidneyPrimary; Dilution: 1 ug/mL
Western blot detection of MSH2 in K562,CEM,Jurkat,293T and A431 cell lysates using MSH2 mouse mAb (1:1000 diluted).Predicted band size:100KDa.Observed band size:100KDa.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA313355 | MSH2 antibody |
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| Rabbit polyclonal anti-MSH2 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, ICC/IF, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| TA324411 | MSH2 (Center) antibody |
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| Rabbit polyclonal MSH2 Antibody (Center) | |||||||
 |
Rabbit | Ig | Aff - Purified | Hu | F, ICC/IF, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
| TA326937 | MSH2 antibody |
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| MSH2 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
|
||
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA327701 | MSH2 antibody |
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| Mouse Monoclonal MSH2 Antibody | |||||||
 |
Mouse | IgG1 | G219-1129 | Purified | Hu | P |
1 ml /
€690.00
|
| OriGene Technologies, Inc. | |||||||
| TA328162 | MSH2 antibody |
||||||
| Mouse monoclonal anti-MSH2 antibody (Center) | |||||||
 |
Mouse | IgG1 | 1184CT1.3.2 | Purified | Hu | WB |
0.4 ml /
€415.00
|
| OriGene Technologies, Inc. | |||||||
| TA347005 | MSH2 antibody |
||||||
| Mouse Monoclonal MSH2 Antibody | |||||||
 |
Mouse | IgG2b | 4G5-E11-D9 | Purified | WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AM06094SU-N | MSH2 antibody |
||||||
 |
Mouse | IgG1 | 1B3=3A2B8C | Ascites | Hu, Mky | E, P, WB |
0.1 ml /
€340.00
|
| OriGene Technologies GmbH | |||||||
| +2 additional images |
|||||||
| AM10116SU-N | MSH2 antibody |
||||||
 |
Mouse | IgG1 | G219-1129 | Supernatant | Hu | P |
1 ml /
€670.00
|
| OriGene Technologies GmbH | |||||||
| AP00168PU-N | MSH2 antibody |
||||||
 |
Rabbit | Aff - Purified | Hu, Ms, Rt | IP, P, WB |
0.1 mg /
€355.00
|
||
| OriGene Technologies GmbH | |||||||
| AP08394PU-N | MSH2 (550-600) antibody |
||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms | P, WB |
50 µg /
€425.00
|
|
| OriGene Technologies GmbH | |||||||
| DM332 | MSH2 antibody |
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|
Mouse | IgG1 | 25D12 | Purified | Hu | P |
1 ml /
€970.00
|
| OriGene Technologies GmbH | |||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN310420 | Msh2 - mouse gene knockout kit via CRISPR |
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|
Mouse | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN310420BN | Msh2 - mouse gene knockout kit via CRISPR |
||
|
Mouse | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN310420LP | Msh2 - mouse gene knockout kit via CRISPR |
||
|
Mouse | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN310420RB | Msh2 - mouse gene knockout kit via CRISPR |
||
|
Mouse | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP305848 | MSH2 |
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| Recombinant protein of human mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli) (MSH2) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€788.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY424843 | MSH2 overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR213288L2V | Lenti ORF particles, Msh2 (GFP-tagged ORF) - Rat mutS homolog 2 (E. coli) (Msh2), 200 uL, >10^7 TU/mL |
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|
200 µl /
€1,790.00
|
|
| OriGene Technologies, Inc. | ||
| RR213288L1V | Lenti ORF particles, Msh2 (Myc-DDK-tagged ORF) - Rat mutS homolog 2 (E. coli) (Msh2), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,610.00
|
|
| OriGene Technologies, Inc. | ||