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NGF antibody

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Principal name

NGF antibody

Alternative names for NGF antibody

Beta-NGF, NGFB,Beta-nerve growth factor

SwissProt ID

P01138 (Human), P01139 (Mouse), P05200 (Chick), P13600 (Bovin), P25427 (Rat), Q29074 (Pig)

Gene ID

4803 (NGF), 18049 (Ngf), 310738

Ncbi ID

NP_002497.2, 9606, NP_002497, 10090, NP_001106168, 10116, NP_001106168.1, ADR82879.1, NM_002506, NP_038637, NM_001112698, NM_001277055, NM_013609

Available reactivities

Rt (Rat), Hu (Human), Bov (Bovine), Ms (Mouse), Viper, Av (Birds)

Available hosts

Goat, Rabbit, Mouse, Sheep

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Functional assay (FN), Immunoprecipitation (IP), Dot blot (Dot)

Background of NGF antibody

The neurotrophins ("neuro" means nerve and "trophe" means nutrient) are a family of soluble, basic growth factors which regulate neurol development, maintence, survival and death in the CNS and the PNS. NGF, the first member of the family to be discovered, was origilly purified as a factor able to support survival of sympathetic and sensory spil neurons in culture. It is synthesized and secreted by sympathetic and sensory target organs and provides trophic support to neurons as they reach their fil target.Neurotrophin secretion also increases in the nervous system following injury. Schwann cells, fibroblasts, and activated mast cells normally synthesize NGF constitutively, however direct trauma and induced cytokines combine to increase neurotrophin production in these cells after injury.NGF is purified in three forms: the 7S, 2.5S and Ã?. The 7S, 130 kDa, form occurs turally in mouse submaxillary glands, and is a multimeric protein composed of two a, one Ã? and two ? subunits. The me is derived from its sedimentation co-efficient, 7S. The biologically active subunit is the Ã?, which is a 26 kDa dimer composed of two identical 120 amino acid chains held together by hydrophobic interactions.5 The 2.5S form is 9 amino acids shorter than the Ã? form, because of proteolysis that occurs during the purification process. The structural hallmark of all the neurotrophins is the characteristic arrangement of the disulfide bridges known as the cysteine knot, which has been found in other growth factors such as PDGF. There is a 95.8% homology between the rat and mouse forms, and a 85% homology between the human and mouse.NGF has been shown to regulate neurol survival, development function and plasticity. Recently, involvement of NGF in processes not involving neurol cells has been shown, such as asthma, psoriasis10 and wound healing. The biological effects of NGF are mediated by two receptors: TrkA, which is specific for NGF, and p75, which binds all the neurotrophins.

PP1057B1 - NGF
Direct ELISA using Biotin conjugated anti-beta-NGF Antibody Cat.-No PP1057B
PP1057B1 - NGF
Sandwich ELISA using Biotin conjugated anti-beta-NGF Antibody Cat.-No PP1057B
PP1057P1 - NGF
Formalin-Fixed, Paraffin-Embedded sections of Human pancreas adenocarcinoma stained with beta-NGF Antibody Cat.-No PP1057P at 0.5 µg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.
PP1057P1 - NGF
Western Blot (Unreduced) using beta-NGF Antibody Cat.-No PP1057P
PP1057P1 - NGF
Western Blot (Reduced) using beta-NGF Antibody Cat.-No PP1057P
PP1057P1 - NGF
Sandwich ELISA using beta-NGF Antibody Cat.-No PP1057P
PAB0756 - NGF
Nerve growth factor ( NGF ) immunoreactivity using sheep Ngf polyclonal antibody ( Cat # PAB0756 ) .
AP14560PU-N - NGF
Formalin-fixed and paraffin-embedded human brain tissue reacted with NGFB antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
PAB7994 - NGF
Immunohistochemical analysis of paraffin-embedded rat brain sections, stain NGF in cytoplasm DAB chromogenic reaction.
PAB7994 - NGF
Western blot analysis of rat brain tissue lysate. Using NGF polyclonal antibody ( Cat # PAB7994 ) .
PAB14337 - NGF
Immunohistochemical staining of nerve growth factor ( Ngf ) in rat cervical ganglion using Ngf polyclonal antibody ( Cat # PAB14337 ).
AP20675PU-N - NGF
Immunohistochemistry analysis of NGF Antibody (Cat.-No AP20675PU-N) in paraffin-embedded human lung carcinoma tissue.
AP20675PU-N - NGF
Western blot analysis of NGF Antibody (Cat.-No AP20675PU-N) in extracts from Jurkat cells.
AP23284PU-N - NGF
Immunohistochemical analysis of paraffin embedded rat tissue sections (brain), staining NGFbeat in cytoplasm, DAB chromogenic reaction
AP23284PU-N - NGF
Western blot analysis of rat brain tissue lysis using NGFbeta antibody
CEK1294 - Human NGF beta ELISA Kit
Representative Standard Curve
CEK1538 - Mouse NGF beta ELISA Kit
Representative Standard Curve
CEK1630 - Rat NGF beta ELISA Kit
Representative Standard Curve
TP321463 - NGF
TP723425 - NGF
TP723426 - NGF
TA321493 - NGF
Predicted band size: 27 kDa . Positive control: Mouse pancreas tissue lysate. Recommended dilution: 1/200-1000. (Gel: 12%SDS-PAGE Lysate: 50 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes)
TA326803 - NGF
Western blot analysis of extracts of mouse liver tissue, using NGF antibody.
TA328404 - NGF
To detect Human beta-NGF by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human beta-NGF.
TA328404 - NGF
To detect Human beta-NGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human beta-NGF (TA328405) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human beta-NGF.
TA328405 - NGF
To detect Human beta-NGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human beta-NGF (TA328404) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human beta-NGF.
TA328405 - NGF
To detect Human beta-NGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human beta-NGF (TA328404) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human beta-NGF.
TA328405 - NGF
To detect Human beta-NGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human beta-NGF (TA328404) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human beta-NGF.
TA328880 - NGF
WB analysis using Anti-mNGF antibody (1:200). Immunohistochemistry Expression of NGF in rat brain. IHC staining of rat hippocampal mossy fiber terminal zone using Anti-mNGF antibody . A. The mossy fiber terminal zone (MF) is seen as the gray area (arrow) adjacent to the pyramidal layer (P). The pyramidal layer is visualized using cresyl violet (purple-labeled cells). B. The antibody was pre-incubated with the immunogen. The lack of staining in the MF zone (arrow) demonstrates specificity.
TA328880 - NGF
Expression of NGF in rat brain. Immunohistochemical staining of rat hippocampal mossy fiber terminal zone using Anti-mNGF antibody. A. The mossy fiber terminal zone (MF) is seen as the gray area (arrow) adjacent to the pyramidal layer (P). The pyramidal layer is visualized using cresyl violet (purple-labeled cells). B. The antibody was pre-incubated with the immunogen. The lack of staining in the MF zone (arrow) demonstrates specificity.
TA328903 - NGF
Western blot analysis of proNGF (WT-mouse) , (Lanes 1-3) and proNGF (WT-human) , (Lanes 4-6): 1, 4. 100 ng + Anti-proNGF antibody (ANT-005), (1:200). 2, 5. 250 ng + Anti-proNGF antibody (1:200). 3, 6. 500 ng + Anti-proNGF antibody (1:200).
TA328903 - NGF
Western blot analysis of mouse submanibular glands (SMGs): 1. Anti-proNGF antibody , (1:200). 2. Anti-proNGF antibody, preincubated with the control peptide antigen.
TA328903 - NGF
Expression of proNGF in rat brain sections. Immunohistochemical staining of proNGF in rat brain sections using Anti-proNGF antibody . A. Staining of astrocytes was performed using mouse anti-glial fibrillary acidic protein (GFAP), labeled red. B. Co-localization between GFAP and proNGF (orange). C. Staining of proNGF (green).
TA347902 - NGF
Western blot analysis of NGF beta expression in Jurkat cells
TA349414 - NGF
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (NGF Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200)
TA349414 - NGF
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (NGF Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200)
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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NGF antibody
Formalin-Fixed, Paraffin-Embedded sections of Human pancreas adenocarcinoma stained with beta-NGF Antibody Cat.-No PP1057P at 0.5 µg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. Rabbit Aff - Purified Hu E, FN, P, WB
50 µg / €250.00
  OriGene Technologies GmbH
+3 additional images

Published recently!

Highly validated antibody!

NGF antibody
Formalin-Fixed, Paraffin-Embedded sections of Human pancreas adenocarcinoma stained with beta-NGF Antibody Cat.-No PP1057P at 0.5 µg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. Rabbit Aff - Purified Hu E, FN, P, WB
0.1 mg / €340.00
  OriGene Technologies GmbH
+3 additional images

Published recently!

Highly validated antibody!

NGF antibody
  Anti-NGF Rabbit Polyclonal Antibody  
Predicted band size: 27 kDa . Positive control: Mouse pancreas tissue lysate. Recommended dilution: 1/200-1000. (Gel: 12%SDS-PAGE Lysate: 50 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

NGF antibody
  Rabbit anti-NGF Polyclonal Antibody  
Western blot analysis of extracts of mouse liver tissue, using NGF antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €335.00
  OriGene Technologies, Inc.

NGF antibody
  Biotinylated Anti-Human ß-NGF Rabbit Polyclonal Antibody  
To detect Human beta-NGF by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human beta-NGF. Rabbit IgG Purified Hu E, WB
50 µg / €275.00
  OriGene Technologies, Inc.
+1 additional image

NGF antibody
  Anti-Human ß-NGF Rabbit Polyclonal Antibody  
To detect Human beta-NGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human beta-NGF (TA328404) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human beta-NGF. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €275.00
  OriGene Technologies, Inc.
+2 additional images

NGF antibody
  Rabbit Polyclonal Anti-mNGF  
WB analysis using Anti-mNGF antibody (1:200). Immunohistochemistry Expression of NGF in rat brain. IHC staining of rat hippocampal mossy fiber terminal zone using Anti-mNGF antibody . A. The mossy fiber terminal zone (MF) is seen as the gray area (arrow) adjacent to the pyramidal layer (P). The pyramidal layer is visualized using cresyl violet (purple-labeled cells). B. The antibody was pre-incubated with the immunogen. The lack of staining in the MF zone (arrow) demonstrates specificity. Rabbit Purified Hu, Ms, Rt WB
0.2 ml / €585.00
  OriGene Technologies, Inc.
+1 additional image

NGF antibody
  Rabbit polyclonal Anti-proNGF  
Western blot analysis of proNGF (WT-mouse) , (Lanes 1-3) and proNGF (WT-human) , (Lanes 4-6): 1, 4. 100 ng + Anti-proNGF antibody (ANT-005), (1:200). 2, 5. 250 ng + Anti-proNGF antibody (1:200). 3, 6. 500 ng + Anti-proNGF antibody (1:200). Rabbit Purified Hu, Ms, Rt WB
0.2 ml / €585.00
  OriGene Technologies, Inc.
+2 additional images

NGF antibody
  Rabbit Polyclonal Anti-NGF beta Antibody  
Western blot analysis of NGF beta expression in Jurkat cells Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €335.00
  OriGene Technologies, Inc.

NGF antibody
  Rabbit Polyclonal Anti-NGF Antibody  
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (NGF Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

NGF antibody
NGF Mouse IgG2b Purified Hu E, FN, WB
0.5 mg / €220.00
  OriGene Technologies GmbH

NGF antibody
NGF Rabbit IgG Purified Hu, Ms E, FN, WB
1 mg / €510.00
  OriGene Technologies GmbH

NGF antibody
Formalin-fixed and paraffin-embedded human brain tissue reacted with NGFB antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P
0.4 ml / €415.00
  OriGene Technologies GmbH

NGF antibody
Immunohistochemistry analysis of NGF Antibody (Cat.-No AP20675PU-N) in paraffin-embedded human lung carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €370.00
  OriGene Technologies GmbH
+1 additional image

NGF antibody
Immunohistochemical analysis of paraffin embedded rat tissue sections (brain), staining NGFbeat in cytoplasm, DAB chromogenic reaction Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €390.00
  OriGene Technologies GmbH
+1 additional image

NGF antibody
Direct ELISA using Biotin conjugated anti-beta-NGF Antibody Cat.-No PP1057B Rabbit Biotin Hu E, WB
25 µg / €250.00
  OriGene Technologies GmbH
+1 additional image

NGF antibody
Direct ELISA using Biotin conjugated anti-beta-NGF Antibody Cat.-No PP1057B Rabbit Biotin Hu E, WB
50 µg / €340.00
  OriGene Technologies GmbH
+1 additional image

NGF antibody
NGF Goat Aff - Purified Rt C, E, P, WB
0.1 mg / €450.00
  Neuromics Antibodies

CRISPR

Catalog No. Species Donor Vector  

Ngf - mouse gene knockout kit via CRISPR
Ngf - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ngf - mouse gene knockout kit via CRISPR
Ngf - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

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