NSF antibody
Principal name
NSF antibody
Alternative names for NSF antibody
Vesicle-fusing ATPase, N-ethylmaleimide-sensitive fusion protein, Vesicular-fusion protein NSF
Ncbi ID
NP_006169.2, NP_068516, 9606, XP_001128468, 10116, 10090, NP_006169, NP_032766.2, NM_008740, NM_021748
Available reactivities
Available hosts
Available applications
Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Enzyme Immunoassay (E), Frozen Sections (C)
Background of NSF antibody
The N-ethylmaleimide-sensitive fusion protein (NSF), acting in concert with SNAPs (Soluble NSF attachment proteins) and SNARES (SNAP receptors), is required for the fusion of transport vesicles to their target membranes in synaptic transmission, intra-Golgi transport, endosome-to-endosome fusion and transcytotic vesicles-toplasma membrane transport. In its functional form, NSF is a homotrimer of 76-kDa subunits. Each subunit contains an amino-terminal domain (N) and two homologous ATP-binding domains (D1 and D2). Vesicle-to-target membrane docking occurs when the vesicle SNARE binds to its cognate target membrane SNARE. a-SNAP (a- or b-SNAP in brain) then binds to this docking complex and mediates the binding of NSF and thus the formation of a 20S fusion particle. It is thought that, once NSF is bound, ATP hydrolysis by NSF initiates the fusion process.
Western Blot analysis of NSF expression in transfected 293T cell line (H00004905-T01) by NSF MaxPab polyclonal antibody.Lane 1: NSF transfected lysate(81.84 KDa).Lane 2: Non-transfected lysate.
Figure 1. A typical Western blot (10% SDS-PAGE) of 3T3 (Lane 1), PC12 (Lane 2), MDBK (Lane 3) and normal human fibroblasts (Lane 4) probed with clone NSF-1 anti-NSF at 0.5 mg/ml. The diffuse bands at ~70 kDa were also present on a blot incubated with second antibody alone (data not shown).
Western Blot analysis of NSF expression in transfected 293T cell line (H00004905-T01) by NSF MaxPab polyclonal antibody.Lane 1: NSF transfected lysate(81.84 KDa).Lane 2: Non-transfected lysate.
Mouse brain stem; . Sample Type :. Mouse brain stem cells. Primary Antibody Dilution :. 1:500. Secondary Antibody :. Goat anti-rabbit Alexa-Fluor 594. Secondary Antibody Dilution :. 1:1000. Color/Signal Descriptions :. NSF: Red DAPI:Blue. Gene Name :. NSF. Submitted by :. Dr. Yuzhi Chen, University of Arkansas for Medical Science; NSF antibody - C-terminal region (ARP40629_P050) in Mouse brain stem cells using Immunohistochemistry
Mouse Brain; . Sample Type: 1. NT-2 cells. 2. mouse brain extracts. Primary Antibody Dilution: 2ug/ml. Secondary Antibody Dilution: 1: 20,000. Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience. Lane 1: 60ug/lane. Lane 2: 80ug/lane. Image Submitted by: Yuzhi Chen. University of Arkansas for Medical Science .; NSF antibody - C-terminal region (ARP40629_P050) in Mouse Brain cells using Western Blot
Human brain; WB Suggested Anti-NSF Antibody Titration: 0.2-1 ug/ml. Positive Control: Human brain; NSF antibody - C-terminal region (ARP40629_P050) in Human brain cells using Western Blot
Western blot analysis of 3T3 (lane 1), PC-12 (lane 2), MDBK (lane 3) and normal human fibroblasts (lane 4) probed with NSF monoclonal antibody, clone NSF-1 ( Cat # MAB1966 ) at 0.5 µg/ml.
Western Blot analysis of NSF expression in transfected 293T cell line (H00004905-T02) by NSF MaxPab polyclonal antibody.Lane 1: NSF transfected lysate(82.60 KDa).Lane 2: Non-transfected lysate.
Western blot of rat caudate lysate showing specific labeling of the ~75k Nsf protein. Using Nsf polyclonal antibody ( Cat # PAB9620 ).
Figure 1. Western blot of rat caudate lysate showing specific labeling of the ~75k NSF protein.
Rat Brain: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot analysis of NSF expression in MCF7 (A); Raw264.7 (B); H9C2 (C) whole cell lysates.
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-NSF Rabbit Polyclonal antibody. (TA590218)
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-NSF Rabbit Polyclonal antibody. (TA590218)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NSF Rabbit Polyclonal antibody. (TA590218)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-NSF Rabbit Polyclonal antibody. (TA590218)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-NSF Rabbit Polyclonal antibody. (TA590218)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-NSF Rabbit Polyclonal antibody. (TA590218)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NSF polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NSF (RC205983, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NSF.
ELISA: NSF Antibody
Western blot analysis of extracts from CoLo cells, using NSF antibody.The lane on the right is treated with the synthesized peptide.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA314288 | NSF antibody |
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| Rabbit polyclonal anti-NSF antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP08714PU-N | NSF (C-term) antibody |
||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | WB |
0.1 ml /
€405.00
|
|
| OriGene Technologies GmbH | |||||||
| AP31259PU-N | NSF antibody |
||||||
 |
Rabbit | IgG | Purified | Ms, Rt | IP, P, WB |
50 µg /
€425.00
|
|
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP305983 | NSF |
||||
| Recombinant protein of human N-ethylmaleimide-sensitive factor (NSF) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR210421L2V | Lenti ORF particles, Nsf (GFP-tagged) - Mouse N-ethylmaleimide sensitive fusion protein (Nsf), 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€1,530.00
|
|
| OriGene Technologies, Inc. | ||
| MR210421L1V | Lenti ORF particles, Nsf (Myc-DDK-tagged) - Mouse N-ethylmaleimide sensitive fusion protein (Nsf), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,370.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR203702L2V | Lenti ORF particles, Nsf (GFP-tagged ORF) - Rat N-ethylmaleimide-sensitive factor (Nsf), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,530.00
|
|
| OriGene Technologies, Inc. | ||
| RR203702L1V | Lenti ORF particles, Nsf (Myc-DDK-tagged ORF) - Rat N-ethylmaleimide-sensitive factor (Nsf), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,370.00
|
|
| OriGene Technologies, Inc. | ||
