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NSF antibody

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Principal name

NSF antibody

Alternative names for NSF antibody

Vesicle-fusing ATPase, N-ethylmaleimide-sensitive fusion protein, Vesicular-fusion protein NSF

SwissProt ID

P46459 (Human), P46460 (Mouse), Q9QUL6 (Rat)

Gene ID

4905 (NSF), 60355 (Nsf), 18195

Ncbi ID

NP_006169.2, NP_068516, 9606, XP_001128468, 10116, 10090, NP_006169, NP_032766.2, NM_008740, NM_021748

Available reactivities

Ms (Mouse), Rt (Rat), Hu (Human), Bov (Bovine), Chk (Chicken), Can (Canine), Ze (Zebrafish), African clawed frog, Mky (Monkey)

Available hosts

Rabbit, Mouse

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Enzyme Immunoassay (E), Frozen Sections (C)

Background of NSF antibody

The N-ethylmaleimide-sensitive fusion protein (NSF), acting in concert with SNAPs (Soluble NSF attachment proteins) and SNARES (SNAP receptors), is required for the fusion of transport vesicles to their target membranes in synaptic transmission, intra-Golgi transport, endosome-to-endosome fusion and transcytotic vesicles-toplasma membrane transport. In its functional form, NSF is a homotrimer of 76-kDa subunits. Each subunit contains an amino-terminal domain (N) and two homologous ATP-binding domains (D1 and D2). Vesicle-to-target membrane docking occurs when the vesicle SNARE binds to its cognate target membrane SNARE. a-SNAP (a- or b-SNAP in brain) then binds to this docking complex and mediates the binding of NSF and thus the formation of a 20S fusion particle. It is thought that, once NSF is bound, ATP hydrolysis by NSF initiates the fusion process.

H00004905-B01 - NSF
Western Blot analysis of NSF expression in transfected 293T cell line (H00004905-T01) by NSF MaxPab polyclonal antibody.Lane 1: NSF transfected lysate(81.84 KDa).Lane 2: Non-transfected lysate.
AM05267PU-N - NSF
Figure 1. A typical Western blot (10% SDS-PAGE) of 3T3 (Lane 1), PC12 (Lane 2), MDBK (Lane 3) and normal human fibroblasts (Lane 4) probed with clone NSF-1 anti-NSF at 0.5 mg/ml. The diffuse bands at ~70 kDa were also present on a blot incubated with second antibody alone (data not shown).
H00004905-B01P - NSF
Western Blot analysis of NSF expression in transfected 293T cell line (H00004905-T01) by NSF MaxPab polyclonal antibody.Lane 1: NSF transfected lysate(81.84 KDa).Lane 2: Non-transfected lysate.
ARP40629_P050 - NSF
Mouse brain stem; . Sample Type :. Mouse brain stem cells. Primary Antibody Dilution :. 1:500. Secondary Antibody :. Goat anti-rabbit Alexa-Fluor 594. Secondary Antibody Dilution :. 1:1000. Color/Signal Descriptions :. NSF: Red DAPI:Blue. Gene Name :. NSF. Submitted by :. Dr. Yuzhi Chen, University of Arkansas for Medical Science; NSF antibody - C-terminal region (ARP40629_P050) in Mouse brain stem cells using Immunohistochemistry
ARP40629_P050 - NSF
Mouse Brain; . Sample Type: 1. NT-2 cells. 2.  mouse brain extracts. Primary Antibody Dilution: 2ug/ml. Secondary Antibody Dilution: 1: 20,000. Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience. Lane 1: 60ug/lane. Lane 2: 80ug/lane. Image Submitted by: Yuzhi Chen. University of Arkansas for Medical Science .; NSF antibody - C-terminal region (ARP40629_P050) in Mouse Brain cells using Western Blot
ARP40629_P050 - NSF
Human brain; WB Suggested Anti-NSF Antibody Titration: 0.2-1 ug/ml. Positive Control: Human brain; NSF antibody - C-terminal region (ARP40629_P050) in Human brain cells using Western Blot
MAB1966 - NSF
Western blot analysis of 3T3 (lane 1), PC-12 (lane 2), MDBK (lane 3) and normal human fibroblasts (lane 4) probed with NSF monoclonal antibody, clone NSF-1 ( Cat # MAB1966 ) at 0.5 µg/ml.
H00004905-D01P - NSF
Western Blot analysis of NSF expression in transfected 293T cell line (H00004905-T02) by NSF MaxPab polyclonal antibody.Lane 1: NSF transfected lysate(82.60 KDa).Lane 2: Non-transfected lysate.
PAB9620 - NSF
Western blot of rat caudate lysate showing specific labeling of the ~75k Nsf protein. Using Nsf polyclonal antibody ( Cat # PAB9620 ).
AP08714PU-N - NSF
Figure 1. Western blot of rat caudate lysate showing specific labeling of the ~75k NSF protein.
AP31259PU-N - NSF
Rat Brain: Formalin-Fixed, Paraffin-Embedded (FFPE)
CPA1830-100ul - NSF
Western blot analysis of NSF expression in MCF7 (A); Raw264.7 (B); H9C2 (C) whole cell lysates.
TA590218 - NSF
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-NSF Rabbit Polyclonal antibody. (TA590218)
TA590218 - NSF
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-NSF Rabbit Polyclonal antibody. (TA590218)
TA590218 - NSF
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NSF Rabbit Polyclonal antibody. (TA590218)
TA590218 - NSF
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-NSF Rabbit Polyclonal antibody. (TA590218)
TA590218 - NSF
Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-NSF Rabbit Polyclonal antibody. (TA590218)
TA590218 - NSF
Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-NSF Rabbit Polyclonal antibody. (TA590218)
TA590218 - NSF
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NSF polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
TA590218 - NSF
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NSF (RC205983, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NSF.
TA590218 - NSF
ELISA: NSF Antibody
TP305983 - NSF
TA314288 - NSF
Western blot analysis of extracts from CoLo cells, using NSF antibody.The lane on the right is treated with the synthesized peptide.

3 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

NSF antibody
  Rabbit polyclonal anti-NSF antibody  
Western blot analysis of extracts from CoLo cells, using NSF antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

NSF (C-term) antibody
Figure 1. Western blot of rat caudate lysate showing specific labeling of the ~75k NSF protein. Rabbit IgG Aff - Purified Hu, Ms, Rt WB
0.1 ml / €405.00
  OriGene Technologies GmbH

NSF antibody
Rat Brain: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Purified Ms, Rt IP, P, WB
50 µg / €425.00
  OriGene Technologies GmbH

3 Item(s)

per page

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Product Type
  1. Primary Antibody (3)
Target
  1. NSF (3)
Property
  1. C-term (1)
Reactivity
  1. Mouse (Ms) (3)
  2. Rat (Rt) (3)
  3. Human (Hu) (2)
Applications
  1. Western blot / Immunoblot (WB) (3)
  2. Enzyme Immunoassay (E) (1)
  3. Immunoprecipitation (IP) (1)
  4. Paraffin Sections (P) (1)
Presentation
  1. Purified (2)
  2. Aff - Purified (1)
Host
  1. Rabbit (3)
Images
  1. 1 image (3)
Images: Applications
  1. Western blot / Immunoblot (WB) (2)
  2. Paraffin Sections (P) (1)
Manufacturer
  1. OriGene Technologies (3)
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