OSBP antibody
Principal name
OSBP antibody
Alternative names for OSBP antibody
OSBP1, OSBP-1, Oxysterol-binding protein 1
Ncbi ID
9606, NM_002556, NP_002547.1, NP_002547, NP_001028346.1, NM_001033174
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)
Background of OSBP antibody
OSBP (Oxysterol-Binding Protein), also called OSBP1, is a protein that in humans is encoded by the OSBP gene. Im et al. (2005) reported the structure of the full-length yeast oxysterol-binding-protein Osh4, a member of the OSBP-related protein (ORP) family, at 1.5- to 1.9-angstrom resolution in complexes with ergosterol, cholesterol, and 7-, 20- and 25-hydroxy cholesterol. Moreira et al. (2001) refined the localization of the OSBP1 gene to chromosome 11q12.1 by radiation hybrid analysis. Wang et al. (2005) found that OSBP functions as a cholesterol-binding scaffolding protein coordinating the activity of 2 phosphatases to control the extracellular signal-regulated kinase (ERK) signaling pathway. Cytosolic OSBP formed an approximately 440-kD oligomer with a member of the PTPPBS family of tyrosine phosphatases, the serine/threonine phosphatase PP2A, and cholesterol.
General readings
Moreira EF, Jaworski C, Li A, Rodriguez IR.
Molecular and biochemical characterization of a novel oxysterol-binding protein (OSBP2) highly expressed in retina.
J Biol Chem. 2001 May 25;276(21):18570-8. Epub 2001 Jan 26.
PMID: 11278871
OSBP polyclonal antibody ( Cat # PAB6769 ) ( 0.1 µg/ml ) staining of human placenta lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
Human Muscle; WB Suggested Anti-OSBP Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Muscle; OSBP antibody - C-terminal region (ARP60212_P050) in Human Muscle cells using Western Blot
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-OSBP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503140)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-OSBP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503140)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-OSBP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503140)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-OSBP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503140)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-OSBP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503140)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-OSBP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503140)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-OSBP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503140)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-OSBP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503140)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-OSBP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503140)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-OSBP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503140)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-OSBP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503140)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-OSBP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503140)
Anti-OSBP mouse monoclonal antibody (TA503140) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY OSBP(RC204250).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OSBP (RC204250, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OSBP.
HEK293T cells transfected with either RC204250 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-OSBP antibody(TA503140), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-OSBP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503180)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-OSBP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503180)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-OSBP monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OSBP (RC204250, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OSBP.
TA303012 (0.1µg/ml) staining of Human Placenta lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA305774 (2ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
WB Suggested Anti-OSBP Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human Muscle
- Quick-Filters:
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA503140 | OSBP antibody |
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| OSBP mouse monoclonal antibody, clone OTI1F2 (formerly 1F2) | |||||||
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Mouse | IgG1 | OTI1F2 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +14 additional images |
Highly validated antibody!
|
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| TA503180 | OSBP antibody |
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| OSBP mouse monoclonal antibody, clone OTI1A4 (formerly 1A4) | |||||||
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Mouse | IgG1 | OTI1A4 | Purified | Hu | P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
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| TA303012 | OSBP antibody |
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| Goat Polyclonal Antibody against OSBP | |||||||
 |
Goat | Aff - Purified | Can, Chimp, Hu, Ms, Rb, Rt | WB |
0.1 mg /
€360.00
|
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| OriGene Technologies, Inc. | |||||||
| TA305774 | OSBP (C-term) antibody |
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| Goat Anti-OSBP1 (C Terminus) Antibody | |||||||
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Goat | Aff - Purified | Hu | WB |
0.1 mg /
€360.00
|
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| OriGene Technologies, Inc. | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP304250 | OSBP |
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| Recombinant protein of human oxysterol binding protein (OSBP) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY419260 | OSBP overexpression lysate |
|
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0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR215637L2V | Lenti ORF particles, Osbp (GFP-tagged) - Mouse oxysterol binding protein (Osbp), 200 uL, >10^7 TU/mL |
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200 µl /
€1,610.00
|
|
| OriGene Technologies, Inc. | ||
| MR215637L1V | Lenti ORF particles, Osbp (Myc-DDK-tagged) - Mouse oxysterol binding protein (Osbp), 200 uL, >10^7 TU/mL |
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200 µl /
€1,450.00
|
|
| OriGene Technologies, Inc. | ||