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OSBP antibody

Principal name

OSBP antibody

Alternative names for OSBP antibody

OSBP1, OSBP-1, Oxysterol-binding protein 1

SwissProt ID

P16258 (Rabit), P22059 (Human), Q3B7Z2 (Mouse)

Gene ID

5007 (OSBP), 76303 (Osbp)

Ncbi ID

9606, NM_002556, NP_002547.1, NP_002547, NP_001028346.1, NM_001033174

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Chimp (Chimpanzee), Rb (Rabbit), Bov (Bovine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Ze (Zebrafish)

Available hosts

Goat, Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of OSBP antibody

OSBP (Oxysterol-Binding Protein), also called OSBP1, is a protein that in humans is encoded by the OSBP gene. Im et al. (2005) reported the structure of the full-length yeast oxysterol-binding-protein Osh4, a member of the OSBP-related protein (ORP) family, at 1.5- to 1.9-angstrom resolution in complexes with ergosterol, cholesterol, and 7-, 20- and 25-hydroxy cholesterol. Moreira et al. (2001) refined the localization of the OSBP1 gene to chromosome 11q12.1 by radiation hybrid analysis. Wang et al. (2005) found that OSBP functions as a cholesterol-binding scaffolding protein coordinating the activity of 2 phosphatases to control the extracellular signal-regulated kinase (ERK) signaling pathway. Cytosolic OSBP formed an approximately 440-kD oligomer with a member of the PTPPBS family of tyrosine phosphatases, the serine/threonine phosphatase PP2A, and cholesterol.

General readings

Moreira EF, Jaworski C, Li A, Rodriguez IR.
Molecular and biochemical characterization of a novel oxysterol-binding protein (OSBP2) highly expressed in retina.
J Biol Chem. 2001 May 25;276(21):18570-8. Epub 2001 Jan 26.
PMID: 11278871

OSBP polyclonal antibody ( Cat # PAB6769 ) ( 0.1 µg/ml ) staining of human placenta lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.

PAB6769

Human Muscle; WB Suggested Anti-OSBP Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Muscle; OSBP antibody - C-terminal region (ARP60212_P050) in Human Muscle cells using Western Blot

ARP60212_P050

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-OSBP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503140)

TA503140

Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-OSBP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503140)

TA503140

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-OSBP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503140)

TA503140

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-OSBP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503140)

TA503140

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-OSBP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503140)

TA503140

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-OSBP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503140)

TA503140

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-OSBP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503140)

TA503140

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-OSBP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503140)

TA503140

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-OSBP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503140)

TA503140

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-OSBP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503140)

TA503140

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-OSBP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503140)

TA503140

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-OSBP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503140)

TA503140

Anti-OSBP mouse monoclonal antibody (TA503140) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY OSBP(RC204250).

TA503140

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OSBP (RC204250, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OSBP.

TA503140

HEK293T cells transfected with either RC204250 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-OSBP antibody(TA503140), and then analyzed by flow cytometry.