PDE4B antibody
Principal name
PDE4B antibody
Alternative names for PDE4B antibody
PDE4B, DPDE4, cAMP-specific 3',5'-cyclic phosphodiesterase 4B, EC=3.1.4.17, PDE32
Ncbi ID
9606, NP_001032416.1, NP_001032416, NM_001037339, NP_001032418, NP_002591, NM_017031, NM_002600, NM_001037340
Available reactivities
Available hosts
Available applications
Frozen Sections (C), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Flow Cytometry (F)
Background of PDE4B antibody
Enzymes of the cAMP-dependent phosphodiesterase type 4 (PDE4) family are important in hydrolyzing cAMP produced by G-protein coupled receptor (GPCR) stimulated adenylyl cyclases. Four genes (PDE4A, B, C and D) make up the PDE4 family. cAMP-dependent phosphodiesterase type B (PDE4B) family is consists of 4 known splice variants, PDE4B1, B2, B3 and a novel 66 kDa PDE4B4 protein (1, 2). One or more subtype variants of PDE4B are ubiquitously present in all mammalian cells. In CNS, all four PDE4B subtype variants are expressed in varying ratios and their activity is regulated in tandem with GPCRs stimulation. Some PDE4B variants are also substrate for PKA-dependent phosphorylation. Peripheral tissues exhibit differential expression of PDE4B1, B2 and B4 variants; the PDE4B4 (66kDa protein) is the major PDE4B variant expressed in lungs, testis and heart. In testis PDE4B enzymes are expressed in somatic cells (Sertoli and Leydig cells) while PDE4A is localized in germ cells (2).
PDE4B2 variant (b) contains a distinct 5’ UTR and lacks an in-frame portion of the 5’ coding region, compared to variant a. The resulting isoform, PDE4B2 (PDE32) has a shorter and distinct N-terminus when compared to isoform 1. Altered activity of this protein has been associated with schizophrenia and bipolar affective disorder. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The PDE4B2 expression is found in brain, heart, lung and skeletal muscle but not in placenta, liver, kidney or pancrease.
General readings
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Souza, R.P., et al. Int Clin Psychopharmacol 25(5):264-269(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010)
Joslyn, G., et al. Alcohol. Clin. Exp. Res. 34(5):800-812(2010)
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Western blot using Scottish AP00656SU-N (1:2,000) against lysate from COS cells transfected with the indicated human PDE isoform.
HEK293 overexpressing human PDE4B and probed with PDE4B polyclonal antibody ( Cat # PAB6965 ) ( mock transfection in first lane ), tested by Origene.
PDE4B polyclonal antibody ( Cat # PAB6965 ) ( 0.5 µg/ml ) staining of mouse brain Lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence
Western blot of PDE4B3 (AP10225PU-N, 1:350) using Mock transfected or PDE4B3 transfected Cos Cells. Light band in mock transfected cells is endogenous PDE4B3.
Human Heart; WB Suggested Anti-PDE4B Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human heart; PDE4B antibody - middle region (ARP56256_P050) in Human Heart cells using Western Blot
Immunohistochemistry Image: Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western Blot Image: Antibody (0.5 µg/ml) staining of Mouse Brain Lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot analysis of PDE4B Antibody (Center) Cat.-No AP53236PU-N in Ramos cell line lysates (35ug/lane). This demonstrates the PDE4B antibody detected the PDE4B protein (arrow).
Flow cytometric analysis of Ramos cells using using PDE4B Antibody (Center) Cat.-No AP53236PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
AP31948PU-N PDE4B antibody staining of Human Uterus lysate at 2 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemical analysis of PDE4B/C/D staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of PDE4B/C/D expression in HeLa (A) whole cell lysates.
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PDE4B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503439)
Anti-PDE4B mouse monoclonal antibody (TA503439) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDE4B(RC211956).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE4B (RC211956, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE4B.
HEK293T cells transfected with either RC211956 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PDE4B antibody(TA503439), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE4B (RC211956, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE4B.
HEK293T cells transfected with either RC211956 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PDE4B antibody(TA503446), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PDE4B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503471)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PDE4B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503471)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PDE4B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503471)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PDE4B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503471)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PDE4B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503471)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PDE4B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503471)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PDE4B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503471)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PDE4B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503471)
Anti-PDE4B mouse monoclonal antibody (TA503471) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDE4B(RC211956).
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PDE4B monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE4B (RC211956, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE4B.
Flow cytometric Analysis of Hela cells, using anti-PDE4B antibody(TA503471),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-PDE4B antibody(TA503471),(Red), compared to a nonspecific negative control antibody,(Blue).
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PDE4B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503477)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PDE4B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503477)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE4B (RC211956, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE4B.
HEK293T cells transfected with either RC211956 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PDE4B antibody(TA503477), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE4B (RC211956, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE4B.
HEK293T cells transfected with either RC211956 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PDE4B antibody(TA503478), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PDE4B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503532)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PDE4B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503532)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PDE4B monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE4B (RC211956, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE4B.
HEK293T cells transfected with either RC211956 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PDE4B antibody(TA503532), and then analyzed by flow cytometry.
TA302571 (0.5µg/ml) staining of Mouse Brain Lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Sample (30 ug of whole cell lysate)Â . A: NT2D1. B:Â PC-3. 7.5% SDS PAGEÂ . TA308931 diluted at 1:1000. Â
PDE4B antibody detects PDE4B protein by western blot analysis. Whole cell extracts (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PDE4B antibody (TA308931) diluted by 1:1000.
WB Suggested Anti-PDE4B Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Human heart
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP31948PU-N | PDE4B (C-term) antibody |
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 |
Goat | Aff - Purified | Hu, Ms | E, WB |
0.1 mg /
€380.00
|
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| OriGene Technologies GmbH | |||||||
OriGene recommends this product for Western blotting. |
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| TA302571 | PDE4B antibody |
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| Goat Polyclonal Antibody against Phosphodiesterase 4B | |||||||
 |
Goat | Aff - Purified | Hu, Ms, Rt | WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA308931 | PDE4B antibody |
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| Rabbit polyclonal antibody to Phosphodiesterase 4B (phosphodiesterase 4B, cAMP-specific (phosphodiesterase E4 dunce homolog, Drosophila)) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu | WB |
0.1 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| AP00656SU-N | PDE4B antibody |
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 |
Rabbit | Serum | Hu, Ms, Rt | C, IP, WB |
0.1 ml /
€440.00
|
||
| OriGene Technologies GmbH | |||||||
| AP23040PU-N | PDE4B (723-736) antibody |
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 |
Goat | Aff - Purified | Hu, Ms | E, P, WB |
50 µg /
€425.00
|
||
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| AP53236PU-N | PDE4B (Center) antibody |
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 |
Rabbit | Ig | Aff - Purified | Hu | F, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| AP55408PU-N | PDE4B cAMP- antibody |
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 |
Rabbit | Purified | Hu | C, E, WB |
0.1 mg /
€450.00
|
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| OriGene Technologies GmbH | |||||||