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PDE9A antibody

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Principal name

PDE9A antibody

Alternative names for PDE9A antibody

Phosphodiesterase 9A, Phosphodiesterase PDE9A21, HSPDE9A2, High affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A

SwissProt ID

O70628 (Mouse), O76083 (Human), Q86WN8 (Human)

Gene ID

5152 (PDE9A), 18585 (Pde9a)

Ncbi ID

NP_001001567, 9606, NP_001001567.1, NP_001001581, NM_002606, NM_001001569, NM_001001571, NM_001001574, NM_001001575, NM_001001576, NM_001001577, NM_001001578, NM_001001582, NM_001001583, NM_001001568, NP_001001578, NP_001001568, NP_001001574, NP_001157220.1, NM_008804, NM_001163748, NM_001001585, NM_001001567, NM_001001573, NM_001001581, NM_001001584, NM_001001572

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Ze (Zebrafish)

Available hosts

Rabbit, Mouse

Available applications

Frozen Sections (C), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of PDE9A antibody

Cyclic nucleotide phosphodiesterases (PDEs) are enzymes which catalyzes the hydrolysis of cyclic adenosine 30,50-monophosphate (cAMP) and/or cyclic guanosine 30,50-monophosphate (cGMP); and PDE9A (high affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A) is a PDE member which hydrolyzes the second messenger cGMP, an important regulator of several critical cellular physiological processes. PDE9A activity gets modulated by metal ions and it can bind 2 divalent metal cations per subunit - site 1 binds zinc ions, whereas, site 2 preferentially associates with magnesium and/or manganese ions. PDE9A is expressed in most of the solid tissues and because of its location as well as contribution to the regulation of steady-state cellular concentrations of cyclic nucleotides, PDE9A has been suggested as a potential candidate for diseases such as bipolar affective disorder and its overexpression has been linked to Down syndrome. Moreover, PDE9A deficient mice fed a high-fat diet have been reported to exhibit a reduced weight gain and fat mass compared with wild-type mice.

General readings

Huai,Q., (2004) Proc. Natl. Acad. Sci. U.S.A. 101 (26), 9624-9629

AP00660SU-N - PDE9A
Western blot using AP00660SU-N (1:2,000) against lysate from Insect cells transfected with the indicated human PDE isoform.
H00005152-M01 - PDE9A
Immunoperoxidase of monoclonal antibody to PDE9A on formalin-fixed paraffin-embedded human cerebellum. [antibody concentration 3 ug/ml]
H00005152-B01P - PDE9A
Immunofluorescence of purified MaxPab antibody to PDE9A on HeLa cell. [antibody concentration 10 ug/ml]
H00005152-B01P - PDE9A
Western Blot analysis of PDE9A expression in transfected 293T cell line (H00005152-T01) by PDE9A MaxPab polyclonal antibody.Lane 1: PDE9A transfected lysate(58.63 KDa).Lane 2: Non-transfected lysate.
ARP45760_P050 - PDE9A
Human kidney; PDE9A Antibody (ARP45760_P050) in Human kidney cells using Immunohistochemistry
ARP45760_P050 - PDE9A
HEK293; . Sample Type :. Lane 1: 5ug in vitro translation without DNA Lane 2: 5ug in vitro translation hNeur1-pcDNA3 Lane 3: 60ug in vitro translation rNeur1-pcDNA3 Lane 4: 60ug HEK293 cell lysate (not transfected) Lane 5: 60ug hNeur1-pcDNA3 overexpressed in HEK293 cells Lane 6: 60ug rNeur1-pcDNA3 overexpressed in HEK293 cells . Primary Antibody Dilution :. 1:1000 . Secondary Antibody:. Anti-rabbit-HRP . Secondary Antibody Dilution:. 1:5000 . Color/Signal Descriptions:. PDE9A . Gene Name:. Kati Taal and Richard Tamme, Department of Gene Technology, Tallinn University of Technology. . Submitted by:.; PDE9A Antibody (ARP45760_P050) in HEK293 cells using Western Blot
ARP45760_P050 - PDE9A
Human Jurkat; WB Suggested Anti-PDE9A Antibody Titration: 1.0 ug/ml. Positive Control: Jurkat cell lysate; PDE9A Antibody (ARP45760_P050) in Human Jurkat cells using Western Blot
ARP45760_P050 - PDE9A
Mouse Brain; WB Suggested Anti-PDE9A Antibody Titration: 5% Milk. ELISA Titer: dilution: 1:500. Positive Control: Mouse Brain lysate; PDE9A Antibody (ARP45760_P050) in Mouse Brain cells using Western Blot
H00005152-B01 - PDE9A
Immunofluorescence of purified MaxPab antibody to PDE9A on HeLa cell. [antibody concentration 10 ug/ml]
H00005152-B01 - PDE9A
Western Blot analysis of PDE9A expression in transfected 293T cell line (H00005152-T01) by PDE9A MaxPab polyclonal antibody.Lane 1: PDE9A transfected lysate(58.63 KDa).Lane 2: Non-transfected lysate.
H00005152-D01P - PDE9A
Western Blot analysis of PDE9A expression in transfected 293T cell line (H00005152-T02) by PDE9A MaxPab polyclonal antibody.Lane 1: PDE9A transfected lysate(61.70 KDa).Lane 2: Non-transfected lysate.
AP10254PU-N - PDE9A
Western blot using AP10254PU-N (1:500).
AM31702PU-N - PDE9A
Human Adrenal Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)
AM31702PU-N - PDE9A
Human Prostate, Epithelium: Formalin-Fixed, Paraffin-Embedded (FFPE)
AM31702PU-N - PDE9A
Detection limit for recombinant GST tagged PDE9A is 1 ng/ml as a capture antibody.
TP317127 - PDE9A
TP324133 - PDE9A
TP324417 - PDE9A
TA308688 - PDE9A
Sample (50 ug of whole cell lysate). A: Mouse brain. 7.5% SDS PAGE. TA308688 diluted at 1:1000.
TA308688 - PDE9A
Sample (30 ug of whole cell lysate). A: 293T. 7.5% SDS PAGE. TA308688 diluted at 1:3000
TA308688 - PDE9A
Immunohistochemical analysis of paraffin-embedded N87 xenograft, using PDE9A(TA308688) antibody at 1:500 dilution.
TA336701 - PDE9A
Western Blot: PDE9A Antibody [TA336701] - WB analysis of PDE9A in HeLa whole cell lysate.
TA336701 - PDE9A
Immunohistochemistry: PDE9A Antibody [TA336701] - PDE9A antibody was tested in mouse prostate using DAB with hematoxylin counterstain.
TA336701 - PDE9A
Immunocytochemistry/Immunofluorescence: PDE9A Antibody [TA336701] - PDE9A antibody was tested in NIH/3T3 cells with Dylight 488 (green). Nuclei were counterstained with DAPI (blue).
TA341105 - PDE9A
Anti-PDE9A antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Items 1 to 20 of 29 total

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PDE9A antibody
  Rabbit Polyclonal antibody to PDE9A (phosphodiesterase 9A)  
Sample (50 ug of whole cell lysate). A: Mouse brain. 7.5% SDS PAGE. TA308688 diluted at 1:1000. Rabbit IgG Aff - Purified Hu, Ms P, WB
0.1 ml / €415.00
  OriGene Technologies, Inc.
+2 additional images

PDE9A antibody
  Rabbit Polyclonal PDE9A Antibody  
Western Blot: PDE9A Antibody [TA336701] - WB analysis of PDE9A in HeLa whole cell lysate. Rabbit Purified Hu, Ms, Rt C, ICC/IF, IP, P, WB
50 µl / €415.00
  OriGene Technologies, Inc.
+2 additional images

PDE9A antibody
  Rabbit Polyclonal Anti-PDE9A Antibody (Internal)  
PDE9A Rabbit Aff - Purified Hu, Ms, Rt P
50 µg / €425.00
  OriGene Technologies, Inc.

PDE9A antibody
Human Adrenal Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 4E1 Purified Hu E, P, WB
50 µg / €475.00
  OriGene Technologies GmbH
+2 additional images

PDE9A antibody
Western blot using AP00660SU-N (1:2,000) against lysate from Insect cells transfected with the indicated human PDE isoform. Rabbit Serum Hu, Ms, Rt C, IP, WB
0.1 ml / €440.00
  OriGene Technologies GmbH

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PDE9A (transcript variant 13)
  Purified recombinant protein of Homo sapiens phosphodiesterase 9A (PDE9A), transcript variant 13  
PDE9A Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.
 

PDE9A (transcript variant 3)
  Purified recombinant protein of Homo sapiens phosphodiesterase 9A (PDE9A), transcript variant 3  
PDE9A Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.
 

PDE9A (transcript variant 9)
  Purified recombinant protein of Homo sapiens phosphodiesterase 9A (PDE9A), transcript variant 9  
PDE9A Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.
 

Lysates

Catalog No.    

PDE9A overexpression lysate
PDE9A overexpression lysate  
0.1 mg / €495.00
  OriGene Technologies, Inc.

PDE9A overexpression lysate
PDE9A overexpression lysate  
0.1 mg / €495.00
  OriGene Technologies, Inc.

PDE9A overexpression lysate
PDE9A overexpression lysate  
0.1 mg / €495.00
  OriGene Technologies, Inc.

PDE9A overexpression lysate
PDE9A overexpression lysate  
0.1 mg / €495.00
  OriGene Technologies, Inc.

PDE9A overexpression lysate
PDE9A overexpression lysate  
0.1 mg / €495.00
  OriGene Technologies, Inc.

PDE9A overexpression lysate
PDE9A overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.

PDE9A overexpression lysate
PDE9A overexpression lysate  
0.1 mg / €495.00
  OriGene Technologies, Inc.

PDE9A overexpression lysate
PDE9A overexpression lysate  
0.1 mg / €495.00
  OriGene Technologies, Inc.

PDE9A overexpression lysate
PDE9A overexpression lysate  
0.1 mg / €495.00
  OriGene Technologies, Inc.

PDE9A overexpression lysate
PDE9A overexpression lysate  
0.1 mg / €495.00
  OriGene Technologies, Inc.

PDE9A overexpression lysate
PDE9A overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.

PDE9A overexpression lysate
PDE9A overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.

Items 1 to 20 of 29 total

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29 products - FILTER YOUR RESULTS!

Available Filters

Product Type
  1. Lysate (17)
  2. Mouse Lenti ORF Particle (4)
  3. Primary Antibody (5)
  4. Protein | Growth Factor (3)
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  1. Polyclonal (4)
  2. Monoclonal (1)
Target
  1. PDE9A (8)
Reactivity
  1. Human (Hu) (5)
  2. Mouse (Ms) (4)
  3. Rat (Rt) (3)
Applications
  1. Paraffin Sections (P) (4)
  2. Western blot / Immunoblot (WB) (4)
  3. Frozen sections (C) (2)
  4. Immunoprecipitation (IP) (2)
  5. Enzyme Immunoassay (E) (1)
  6. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
Presentation
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  2. IHC (2)
  3. ELISA (capture) (E(capture)) (1)
  4. Immunofluorescence (IF) (1)
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  1. HEK293 cells (3)
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