PEX5L antibody
Principal name
PEX5L antibody
Alternative names for PEX5L antibody
PEX5-related protein, PEX2-related protein, PEX5-like protein, Peroxin-5-related protein, Pex5Rp, PEX5R, PXR2, TRIP8b, Peroxisome biogenesis factor 5-like
Ncbi ID
NM_021483, NP_057643.1, NP_057643, NP_775175.1, NP_001156988.1, 10090, NM_173152, NM_001163517
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of PEX5L antibody
PEX5R (also called TRIP8b, tetratricopetide repeat-containing Rab8b-interacting protein) is a cytoplasmic protein expressed as a family of altertively spliced isoforms. PEX5R /TRIP8b isoforms contain a large constant domain preceded by a variable region. The C-termil half of TRIP8b comprises a tetratricopeptide repeat (TPR) protein binding domain. Alysis of the Pex5p-like protein revealed the presence of a tetratricopeptide repeat (TPR) domain in its C-termil half consisting of seven TPR motifs. Pex5R is almost exclusively expressed in brain. Recent studies provide strong evidence that PEX5R interacts with the carboxyl-termil region of Hyperpolarization-activated cyclic nucleotide-gated (HCN) channels and regulates their cell-surface expression level and cyclic nucleotide dependence. HCN channels are key modulators of neurol activity by providing the depolarizing cation current involved in rhythmogenesis, dendritic integration, and syptic transmission. In contrast, Gosh et al. have shown that human PEX5R can bind to peroxisomal targeting sigl-1 (PTS1) ligands. Furthermore it was shown that enzyme alanine–glyoxylate aminotransferase (AGT) is recognized by PEX5R in the cytoplasm, which allows its subsequent translocation into the peroxisome. TRIP8b may play a role in both normal neurol function and in aberrant neurol excitability associated with neurological diseases.
Mouse Liver; WB Suggested Anti-Pex2 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Mouse Liver; Pex2 antibody - C-terminal region (ARP56944_P050) in Mouse Liver cells using Western Blot
Adult rat hippocampus immunohistochemistry
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged TRIP8b-1a-4, TRIP8b-1b-2, TRIP8b-1a-2 or untagged Kv2.1 plasmid and probed with N212/7 (left) or N86/8 (right) TC supe.
Immunoblot versus crude membranes from adult rat brain (RBM) and WT and TRIP8b KO mouse brains probed with N212/7 (left) and N52A/42 (right) TC supe. Mouse brains courtesy of Dane Chetkovich (Northwestern University).
Adult rat hippocampus immunohistochemistry
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged TRIP8b-1a-4, TRIP8b-1b-2, TRIP8b-1a-2 or untagged Kv2.1 plasmid and probed with N212/7 (left) or N86/8 (right) TC supe.
Immunoblot versus crude membranes from adult rat brain (RBM) and WT and TRIP8b KO mouse brains probed with N212/7 (left) and N52A/42 (right) TC supe. Mouse brains courtesy of Dane Chetkovich (Northwestern University).
Adult rat hippocampus immunohistochemistry
transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged TRIP8b-1a-4, TRIP8b-1b-2, TRIP8b-1a-2 and untagged Kv2.1 plasmids and probed with N212/17 (left) and N86/8 (right) TC supe.
Immunoblot versus crude membranes from adult rat brain (RBM) and WT and TRIP8b KO mouse brains probed with N212/17 (left) and N52A/42 (right) TC supe. Mouse samples courtesy of Dane Chetkovich (Northwestern University).
Adult rat hippocampus immunohistochemistry
transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged TRIP8b-1a-4, TRIP8b-1b-2, TRIP8b-1a-2 and untagged Kv2.1 plasmids and probed with N212/17 (left) and N86/8 (right) TC supe.
Immunoblot versus crude membranes from adult rat brain (RBM) and WT and TRIP8b KO mouse brains probed with N212/17 (left) and N52A/42 (right) TC supe. Mouse samples courtesy of Dane Chetkovich (Northwestern University).
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged TRIP8b-1a-4, TRIP8b-1b-2, TRIP8b-1a-2 or untagged Kv2.1 plasmid and probed with N212A/34 (left) or N86/8 (right) TC supe.
immunoblot versus crude membranes from adult rat brain (RBM) and WT and TRIP8b KO mouse brains probed with N212A/34 (left) and N52A/42 (right) TC supe. Mouse samples courtesy of Dane Chetkovich (Northwestern University).
Immunoblot against crude membranes from whole rat (RBM) and mouse (MBM) brain and from human cerebellum (HBM(Cb)], cerebral cortex [HBM(Cx)] and hippocampus [HBM(H)] probed with N212A/34 (left) or N52A/42 (right) TC supe.
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged TRIP8b-1a-4, TRIP8b-1b-2, TRIP8b-1a-2 or untagged Kv2.1 plasmid and probed with N212A/34 (left) or N86/8 (right) TC supe.
immunoblot versus crude membranes from adult rat brain (RBM) and WT and TRIP8b KO mouse brains probed with N212A/34 (left) and N52A/42 (right) TC supe. Mouse samples courtesy of Dane Chetkovich (Northwestern University).
Immunoblot against crude membranes from whole rat (RBM) and mouse (MBM) brain and from human cerebellum (HBM(Cb)], cerebral cortex [HBM(Cx)] and hippocampus [HBM(H)] probed with N212A/34 (left) or N52A/42 (right) TC supe.
Adult rat hippocampus immunohistochemistry
Adult rat brain immunoblot
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged TRIP8b-1a-4, TRIP8b-1b-2, TRIP8b-1a-2 or untagged Kv2.1 plasmid and probed with N212/3 (left) and N86/8 (right) TC supe.
Immunoblot versus crude membranes from adult rat brain (RBM) and WT and TRIP8b KO mouse brains probed with N212/3 (left) and N52A/42 (right) TC supe. Mouse samples courtesy of Dane Chetkovich (Northwestern University).
Adult rat hippocampus immunohistochemistry
Adult rat brain immunoblot
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged TRIP8b-1a-4, TRIP8b-1b-2, TRIP8b-1a-2 or untagged Kv2.1 plasmid and probed with N212/3 (left) and N86/8 (right) TC supe.
Immunoblot versus crude membranes from adult rat brain (RBM) and WT and TRIP8b KO mouse brains probed with N212/3 (left) and N52A/42 (right) TC supe. Mouse samples courtesy of Dane Chetkovich (Northwestern University).
Transfected cell immunoblot: extracts of COS cells transiently transfected with carboxyterminally (C) and amino-terminally (N) GFP-tagged TRIP8b-1a-5, TRIP8b-1a-4 or untagged Kv2.1 plasmid and probed with N291C/22 (left)and N212/7 (right) TC supe. Immunoblot versus crude membranes from adult rat brain (RBM) and WT and TRIP8b KO mouse brains probed with N291C/22 (left) and N52A/42 (right) TC supe. Mouse brains courtesy of Dane Chetkovich (Northwestern University)
Transfected cell immunoblot: extracts of COS cells transiently transfected with carboxyterminally (C) and amino-terminally (N) GFP-tagged TRIP8b-1a-5, TRIP8b-1a-4 or untagged Kv2.1 plasmid and probed with N291C/22 (left)and N212/7 (right) TC supe. Immunoblot versus crude membranes from adult rat brain (RBM) and WT and TRIP8b KO mouse brains probed with N291C/22 (left) and N52A/42 (right) TC supe. Mouse brains courtesy of Dane Chetkovich (Northwestern University)
Western blot analysis of rat brain (lanes 1 and 3) and mouse brain (lanes 2 and 4) lysates: 1-2. Anti-TRIP8b antibody , (1:400). 3-4. Anti-TRIP8b antibody, preincubated with the control peptide antigen.
WB Suggested Anti-Pex2 Antibody; Titration: 1.0 ug/ml; Positive Control: Mouse Liver
- Quick-Filters:
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| 73-208 | PEX5L antibody |
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Mouse | IgG1 | N212/3 | Supernatant | Hu, Ms, Rt | ICC/IF, WB |
5 ml /
€490.00
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| Antibodies Incorporated | |||||||
 +3 additional images |
Highly validated antibody!
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| 75-208 | PEX5L antibody |
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 |
Mouse | IgG1 | N212/3 | Purified | Hu, Ms, Rt | ICC/IF, WB |
0.1 ml /
€570.00
|
| Antibodies Incorporated | |||||||
| +3 additional images |
Highly validated antibody!
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| TA329011 | PEX5L antibody |
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| Rabbit polyclonal Anti-TRIP8b | |||||||
 |
Rabbit | Purified | Ms, Rt | WB |
0.2 ml /
€585.00
|
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| OriGene Technologies, Inc. | |||||||
| 73-243 | PEX5L (constant) antibody |
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 |
Mouse | IgG2b | N212/7 | Supernatant | Hu, Ms, Rt | C, ICC/IF, WB |
5 ml /
€490.00
|
| Antibodies Incorporated | |||||||
| +2 additional images |
|
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| 73-244 | PEX5L (constant) antibody |
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 |
Mouse | IgG2a | N212/17 | Supernatant | Hu, Ms, Rt | C, ICC/IF, WB |
5 ml /
€490.00
|
| Antibodies Incorporated | |||||||
| +2 additional images |
|
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| 73-245 | PEX5L (exon 1b) antibody |
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 |
Mouse | IgG1 | N212A/34 | Supernatant | Hu, Ms, Rt | ICC/IF, WB |
5 ml /
€490.00
|
| Antibodies Incorporated | |||||||
| +2 additional images |
|
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| 73-334 | PEX5L (exon 1a/5 junction) antibody |
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 |
Mouse | IgG1 | N291C/22 | Supernatant | Ms, Rt | ICC/IF, WB |
5 ml /
€490.00
|
| Antibodies Incorporated | |||||||
|
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| 75-243 | PEX5L (constant) antibody |
||||||
 |
Mouse | IgG2b | N212/7 | Purified | Hu, Ms, Rt | C, ICC/IF, WB |
0.1 ml /
€570.00
|
| Antibodies Incorporated | |||||||
| +2 additional images |
|
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| 75-244 | PEX5L (constant) antibody |
||||||
 |
Mouse | IgG2a | N212/17 | Purified | Hu, Ms, Rt | C, ICC/IF, WB |
0.1 ml /
€570.00
|
| Antibodies Incorporated | |||||||
| +2 additional images |
|
||||||
| 75-245 | PEX5L (exon 1b) antibody |
||||||
 |
Mouse | IgG1 | N212A/34 | Purified | Hu, Ms, Rt | ICC/IF, WB |
0.1 ml /
€570.00
|
| Antibodies Incorporated | |||||||
| +2 additional images |
|
||||||
| 75-334 | PEX5L (exon 1a/5 junction) antibody |
||||||
 |
Mouse | IgG1 | N291C/22 | Purified | Ms, Rt | ICC/IF, WB |
0.1 ml /
€570.00
|
| Antibodies Incorporated | |||||||
|
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP319522 | PEX5L |
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| Recombinant protein of human peroxisomal biogenesis factor 5-like (PEX5L) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR216226L2V | Lenti ORF particles, Pex5l (GFP-tagged) - Mouse peroxisomal biogenesis factor 5-like (Pex5l), transcript variant 1, 200 uL, >10^7 TU/mL |
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200 µl /
€1,350.00
|
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| OriGene Technologies, Inc. | ||
| MR219087L2V | Lenti ORF particles, Pex5l (GFP-tagged) - Mouse peroxisomal biogenesis factor 5-like (Pex5l), transcript variant 3, 200 uL, >10^7 TU/mL |
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200 µl /
€930.00
|
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| OriGene Technologies, Inc. | ||
| MR216226L1V | Lenti ORF particles, Pex5l (Myc-DDK-tagged) - Mouse peroxisomal biogenesis factor 5-like (Pex5l), transcript variant 1, 200 uL, >10^7 TU/mL |
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200 µl /
€1,210.00
|
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| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR213919L2V | Lenti ORF particles, Pex5l (GFP-tagged ORF) - Rat peroxisomal biogenesis factor 5-like (Pex5l), 200 uL, >10^7 TU/mL |
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200 µl /
€1,330.00
|
|
| OriGene Technologies, Inc. | ||
| RR213919L1V | Lenti ORF particles, Pex5l (Myc-DDK-tagged ORF) - Rat peroxisomal biogenesis factor 5-like (Pex5l), 200 uL, >10^7 TU/mL |
|
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200 µl /
€1,190.00
|
|
| OriGene Technologies, Inc. | ||
