PPAR-gamma antibody
Principal name
PPAR-gamma antibody
Alternative names for PPAR-gamma antibody
PPARG, NR1C3, Peroxisome proliferator-activated receptor gamma
SwissProt ID
O18971 (Bovin), O19052 (Rabit), O62807 (Pig), O88275 (Rat), P37231 (Human), Q15180 (Human)
Ncbi ID
NP_005028.4, 9606, NP_056953, NP_005028, NM_015869, NP_619726, NP_619725, NP_001138838.1, NM_013124, NM_001145366, NM_138711, NM_005037
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunohistochemistry (IHC), Electrophoretic Mobility Shift Assay (EMSA), Immunoprecipitation (IP), Frozen Sections (C)
Background of PPAR-gamma antibody
Since their discovery in the early 1990's, the peroxisome proliferator activated receptors (PPARs) have attracted significant attention. This is primarily because PPARs serve as receptors for two very important classes of drugs: the hypolipidemic fibrates and the insulin sensitizing thiazolidinediones. Peroxisome proliferators are non-genotoxic carcinogens that are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed PPARs. Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Upon binding fatty acids or hypolipidemic drugs, PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate the expression of target genes. There are 3 known subtypes of PPARs: PPAR-alpha, PPAR-delta and PPAR-gamma. The target genes of PPAR-alpha and PPAR-delta are primarily involved in the catabolism of fatty acids. Conversely, PPAR-gamma is activated by peroxisome proliferators such as prostaglandins, leukotrienes and anti-diabetic thiazolidinediones and affects the expression of genes involved in the storage of fatty acids. PPAR-gamma may also be involved in adipocyte differentiation. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 through interaction with specific response elements.
General readings
Berger J.. et al. (2002) Annu Rev Med . 53:409-35.
Michalik L .,et al. (2006) Pharmacol Rev. 58(4):726-41.
Human Jurkat; WB Suggested Anti-PPARG Antibody Titration: 1.25ug/ml. ELISA Titer: 1:1562500. Positive Control: Jurkat cell lysate; PPARG antibody - N-terminal region (ARP39207_T100) in Human Jurkat cells using Western Blot
Mouse cornea and eyelid; Mouse cornea and eyelid. Primary Antibody Dilution :. 1:100. Secondary Antibody :. Goat anti-rabbit-AlexaFluor-546. Secondary Antibody Dilution :. 1:1000. Color/Signal Descriptions :. PPARG: Green DAPI: Red. Gene Name :. Ppar Gamma . Submitted by :. Anonymous; PPARG antibody - N-terminal region (ARP32880_T100) in Mouse cornea and eyelid cells using Immunohistochemistry
Human 293T; WB Suggested Anti-PPARG Antibody Titration: 1.25ug/ml. Positive Control: 293T cell lysate; PPARG antibody - N-terminal region (ARP32880_T100) in Human 293T cells using Western Blot
Western blot analysis of PPARgamma in lysate from 3T3 cells (Lane 1) and Jurkat Cells (Lane 2,3) using antibody AP00649PU-N
Immunohistochemistry (IHC) analyzes of PPAR-γ antibody (Cat.-No.: AP06573PU-N) in paraffin-embedded human placenta tissue.
Western blot (WB) analysis of PPAR-γ antibody (Cat.-No.: AP06573PU-N) in extracts from K562 cells.
Formalin-Fixed Paraffin-Embedded Human Prostate stained with PPARG antibody Cat.-No AP07257PU-N at 5 µg/ml followed by biotinylated Goat anti-Rabbit IgG secondary antibody, Alkaline Phosphatase-streptavidin and Chromogen.
Figure 2. Staining PPARG in Heart tissue by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue.
Figure 1. Staining PPARG in Liver tissue by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue.
Western blot using PPARG polyclonal antibody ( Cat # PAB11248 ) shows detection of PPARG protein in a mouse 3T3 whole cell lysate ( lane 1 arrowhead ).Approximately 20 µg of lysate was loaded onto a 4-20% gradient gel followed by transfer to nitrocellulose.Primary antibody was used at a 1 : 2,000 dilution in 5% BLOTTO PBS solution.The membrane was washed and reacted with a 1 : 10,000 dilution of IRDye.IgGTM800 Conjugated Affinity Purified Goat-anti-Rabbit [H&L] MX10.Molecular weight estimation was made by comparison to prestained MW markers indicated at the left ( lane M ).
Western blot analysis of extract from mouse adipose tissue, using PPARG polyclonal antibody ( Cat # PAB8500 ) .
Western blot analysis of PPARG expression in lysate from NIH/3T3 cells ( Lane 1 ) and Jurkat Cells ( Lane 2 and 3 ) .
293T cells were analysed by Western blot using PPARG polyclonal antibody ( Cat # PAB14095 ) diluted 1 : 500. Figure shows the result of 293T cells transfected with pNTAP-PPARG ( Lane 2 ) or with an empty vector ( Lane 1 ).
ELISA was performed using a serial dilution of PPARG polyclonal antibody ( Cat # PAB14095 ). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1 : 27,600.
Human Jurkat; WB Suggested Anti-PPARG Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:500. Positive Control: Jurkat cell lysate; PPARG antibody - N-terminal region (ARP32879_P050) in Human Jurkat cells using Western Blot
Recombinant human PPARG, 209-477 aa, His-tagged
Formalin-Fixed, Paraffin-Embedded Human Adrenal stained with PPARG antibody Cat.-No AM20438PU-N at 1/200 dilution after heat-induced antigen retrieval.
Formalin-Fixed, Paraffin-Embedded Human Prostate stained with PPARG antibody Cat.-No AM20438PU-N at 1/200 dilution after heat-induced antigen retrieval.
Western blot analysis using PPARG antibody Cat.-No AM20438PU-N against truncated PPARG-His recombinant protein (Lane 1) and full-length PPARG (aa1-477) transfected CHO-K1 cell lysate (Lane 2).
Western blot with polyclonal anti- PPAR-G antibody
Western blot analysis using PPARG antibody Cat.-No AM06243SU-N against truncated PPARG-His recombinant protein (Lane 1) and full-length PPARG (aa1-477) transfected CHO-K1 cell lysate (Lane 2).
Western blot analysis of PPAR gamma (pS112) expression in HeLa colchicine-treated (A); Raw264.7 colchicine-treated (B); PC12 colchicine-treated (C); U937 (D) whole cell lysates.
ELISA: PPAR gamma Antibody
Western blot analysis of rat testis lysates with anti-PPARG antibody (1:500).
WB using Anti-PPAR2 antibody shows detection of PPAR2 protein in a mouse 3T3 whole cell lysate (lane 1 arrowhead). Approximately 20 ug of lysate was loaded onto a 4-20% gradient gel followed by transfer to nitrocellulose. Primary antibody was used at a 1:2,000 dilution. The membrane was washed and reacted with a 1:10,000 dilution of IRDye™800 Conjugated Goat-anti-Rabbit IgG [H&L] MX10. Molecular weight estimation was made by comparison to prestained MW markers indicated at the left (lane M).
Predicted cell location: Cytoplasm. Positive control: Human ovarian and thyroid cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human ovarian cancer tissue using PPARG antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x 200)
Predicted cell location: Cytoplasm. Positive control: Human ovarian and thyroid cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human thyroid cancer tissue using PPARG antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x200)
Predicted cell location: Cytoplasm. Positive control: Human ovarian and thyroid cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human ovarian cancer tissue using PPARG antibody at dilution 1/20, on the right is treated with the synthetic peptide. (Original magnification:x 200)
Predicted cell location: Cytoplasm. Positive control: Human ovarian and thyroid cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human thyroid cancer tissue using PPARG antibody at dilution 1/20, on the right is treated with the synthetic peptide. (Original magnification:x200)
Western blot analysis of extracts of various cell lines, using PPARG antibody.
Immunohistochemistry of paraffin-embedded H-ovary using PPARG Antibody at Dilution 1:100
Immunofluorescence analysis of A549 cell using PPARG antibody. Blue: DAPI for nuclear staining.
Western blot analysis of PPAR-gamma expression in HuvEc whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
WB Suggested Anti-PPARG Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 500; Positive Control: Jurkat cell lysate
WB Suggested Anti-PPARG Antibody Titration: 1.25ug/ml; Positive Control: 293T cell lysate
Mouse cornea and eyelid; Primary Antibody Dilution: 1: 100; Secondary Antibody: Goat anti-rabbit-AlexaFluor-546; Secondary Antibody: ilution . 1: 1000; Color/Signal Descriptions . PPARG: Green DAPI: Red; Gene Name: Ppar Gamma Submitted by: Anonymous
Western Blot: PPAR gamma Antibody [TA336911] - Western blot analysis of PPAR gamma in 1. human adipose and 2. human adrenal lysate.
Immunohistochemistry-Paraffin: PPAR gamma Antibody [TA336911] - IHC analysis of PPAR gamma in mouse liver.
WB Suggested Anti-PPARG Antibody Titration: 1.25 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Jurkat cell lysate
Host: Rabbit; Target Name: Pparg; Sample Tissue: Rat Kidney lysates; Antibody Dilution: 1.0 ug/ml
Western blot detection of PPAR gamma (C-terminus) in 3T3-L1 and Mouse Lung cell lysates using PPAR gamma (C-terminus) mouse mAb (1:1000 diluted).Predicted band size:54KDa.Observed band size:54KD.
WB using the antibody against PPARG. The antibody was diluted 1:2,000 in TBS-Tween containing 3% skimmed milk. Image shows the result of 293T cells transfected with pNTAP-PPARG (lane 1) and of non-transfected cells (lane 2). The position of the protein of interest is indicated on the right the marker (in kDa) is shown on the left.
ChIP was performed on macrophages derived from mouse bone marrow using the ab against PPARG and optimized PCR primer sets for qPCR. Sheared chromatin from 1 million cells and 1 ug of PPARg antibody were used per ChIP experiment. IgG was used as a negative IP control. Figure 1A: recovery, expressed as the % of input, of the PDK4 PPAR response element (RE). Figure 1B: recovery of the FABP4 Adipo PPAR RE in cells treated with RSG, a very strong activating ligand of PPARG, and in untreated cells.
Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody against human PPARG. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be 1:70,250.
Western blot analysis of PPAR gamma expression in Hela (A); NIH3T3 (B); PC12 (C) whole cell lysates.
Immunohistochemical analysis of PPAR gamma staining in mouse heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of PPAR- gamma phosphorylation expression in Paclitaxel treated Jurkat whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC212449L1V | Lenti ORF particles, PPARG (Myc-DDK tagged) - Human peroxisome proliferator-activated receptor gamma (PPARG), transcript variant 4 , 200 uL, >10^7 TU/mL |
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200 µl /
€1,040.00
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| OriGene Technologies, Inc. | ||
