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PPP1CA antibody

Principal name

PPP1CA antibody

Alternative names for PPP1CA antibody

PPP1A, PP-1A, Serine/threonine-protein phosphatase PP1-alpha catalytic subunit

SwissProt ID

P62136 (Human), P62137 (Mouse), P62138 (Rat), P62139 (Rabit), Q3T0E7 (Bovin), Q8WMS6 (Canfa)

Gene ID

5499 (PPP1CA), 100009298, 19045 (Ppp1ca), 24668

Ncbi ID

9606, NP_001008709.1, NP_002699, NP_001095176, NP_001008709, NM_002708, NM_001008709, NP_114074.1, NM_031868, NM_031527, NM_206873

Available reactivities

Hu (Human), Bov (Bovine), Ms (Mouse), Rt (Rat), Can (Canine), Por (Porcine), Rb (Rabbit), Eq (Equine), GP (Guinea Pig)

Available hosts

Mouse, Rabbit, Sheep

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Frozen Sections (C)

Background of PPP1CA antibody

PPP1CA is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory.The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene.

General readings

1. Barker, H. M.; Jones, T. A.; da Cruz e Silva, E. F.; Spurr, N. K.; Sheer, D.; Cohen, P. T. W.: Localization of the gene encoding a type I protein phosphatase catalytic subunit to human chromosome band 11q13. Genomics 7: 159-166, 1990.
2 Genoux, D.; Haditsch, U.; Knobloch, M.; Michalon, A.; Storm, D.; Mansuy, I. M. : Protein phosphatase 1 is a molecular constraint on learning and memory. Nature 418: 970-975, 2002.

PPP1CA polyclonal antibody (A01), Lot # 060102JCO1 Western Blot analysis of PPP1CA expression in HL-60 ( Cat # L014V1 ).

H00005499-A01

Immunohistochemistry: AM03118PU-N PPP1CA antibody staining of Formalin-Fixed, Paraffin-Embedded Human Brain, Cortex at 5 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Immunohistochemistry: AM03118PU-N PPP1CA antibody staining of Formalin-Fixed, Paraffin-Embedded Human Adrenal at 5 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Western blot analysisCell lysates of HepG2 (45 µg) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PPP1CA (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Cell lysates of HepG2 (45ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PPP1CA (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

PP1 alpha antibody western blot of total rat brain homogenate. (+) indicates staining done in the presence of specific blocking peptide or (-) Peptide Control.

AP05032PU-N

Western blot analysis of cell lysates of HepG2 ( 45 µg ) were resolved by SDS - PAGE , transferred to NC membrane and probed with PPP1CA monoclonal antibody , clone 4G3 ( 1 : 1000 ) ( Cat # MAB1112 ) . Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.

MAB1112

Western blot of PPP1CA polyclonal antibody ( Cat # PAB10410 ) from total rat brain homogenate. (+) indicates staining done in the presence of specific blocking peptide or (-) peptide control.

PAB10410

Immunoblot analysis of cell lysates.Lane 1 : Jurkat cell lysate. Lnae 2 : MOLT 4 cell lysate.Lane 3 : HT-29 cell lysate.Lane 4 : A-431 cell lysate.Lane 5 : SK-N-SH cell lysate.Lane 6 : PC-12 cell lysate.Lane 7 : NIH/3T3 cell lysate.

MAB2728

HEK293, H1299; WB Suggested Anti-PPP1CA Antibody . . Positive Control: Lane 1: 40ug HEK293 lysate Lane 2: 40ug H1299 lysate . Primary Antibody Dilution : 1:1000. Secondary Antibody : Goat anti-rabbit-HRP . Secondry Antibody Dilution : 1:5000. Submitted by: Jose Luis Rosa, Universitat de Barcelona; PPP1CA antibody - N-terminal region (ARP58652_P050) in HEK293, H1299 cells using Western Blot

ARP58652_P050

Human Jurkat; WB Suggested Anti-PPP1CA Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Jurkat cell lysate. PPP1CA is supported by BioGPS gene expression data to be expressed in Jurkat.; PPP1CA antibody - N-terminal region (ARP58652_P050) in Human Jurkat cells using Western Blot

ARP58652_P050

Immunohistochemistry analyzes of PP1α antibody (Cat.-No.: AP20315PU-N) in paraffin-embedded human breast carcinoma tissue.

AP20315PU-N

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PP1-alpha (Phospho-Thr320) Antibody. The picture on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using PP1-alpha (Ab-320) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from Jurkat cells, using PP1-alpha (Ab-320) Antibody. The lane on the right is treated with the synthesized peptide.

PPP1CA, 1-330 aa, Human, His-tagged

Western blot analysis using PPP1A antibody Cat.-No AM06514SU-N against Hela (1) and NIH/3T3 (2) cell lysate.

AM06514SU-N

Western blot analysis using PPP1CA mouse mAb against Hela (1), HepG2 (2), MCF-7 (3), Jurkat (4) and A549 (5) cell lysate.

AM06712SU-N

Western blot analysis using PPP1CA mAb against human PPP1CA (AA: 174-330) recombinant protein. (Expected MW is 38 kDa)

AM06712SU-N

Red: Control Antigen (100ng)Purple: Antigen (10ng)Green: Antigen (50ng)Blue: Antigen (100ng)

AM06712SU-N

Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-PPP1CA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808819)(1:150)

TA808819

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PPP1CA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808819)(1:150)

TA808819

Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-PPP1CA mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808819)(1:150)

TA808819

Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-PPP1CA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808819)(1:150)

TA808819

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PPP1CA mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808819)(1:150)

TA808819

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PPP1CA mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808819)(1:150)

TA808819

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PPP1CA mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808819)(1:150)

TA808819

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PPP1CA mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808819)(1:150)

TA808819

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPP1CA (RC212268, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPP1CA.(1:2000)

TA808819

TP760246

TP760285

Predicted band size: 38 kDa . Positive control: HeLa cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

TA323467

Western blot analysis of extracts of various cell lines, using PPP1CA antibody.

TA327322

Immunohistochemistry of paraffin-embedded human kidney using PPP1CA antibody at dilution of 1:200 (400x lens).

TA327322

Immunohistochemistry of paraffin-embedded human stomach cancer using PPP1CA antibody at dilution of 1:200 (400x lens).

TA327322

Immunofluorescence analysis of U2OS cell using PPP1CA antibody. Blue: DAPI for nuclear staining.

TA327322

Host: Rabbit; Target Name: Ppp1ca; Sample Tissue: Mouse Spleen lysates; Antibody Dilution: 1.0 ug/ml

TA343228

WB Suggested Anti-PPP1CA Antibody ; Positive Control: Lane 1: 40ug HEK293 lysate Lane 2: 40ug H1299 lysate; Primary Antibody Dilution : 1:1000; Secondary Antibody : Goat anti-rabbit-HRP; Secondry Antibody Dilution : 1:5000; Submitted by: Jose Luis Rosa, U

TA344247

WB Suggested Anti-PPP1CA Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Jurkat cell lysatePPP1CA is supported by BioGPS gene expression data to be expressed in Jurkat

TA344247

Western blot detection of PPP1A in MCF7,K562,Hela,HEPG2 and Jurkat whole cell lysates using PPP1A mouse mAb (1:1000 diluted).Predicted band size: 38KDa.Observed band size: 38KDa.

TA347021

IHC of paraffin-embedded human breast cancer using anti-PPP1A mouse mAb diluted 1/500-1/1000.

TA347021

Immunocytochemistry stain of Hela using PPP1A mouse mAb (1:300).

TA347021

Western blot detection of PPP1A in HepG2,MCF7 and Hela cell lysates using PPP1A mouse mAb (1:1000 diluted).Predicted band size:37KDa.Observed band size:37KDa.

TA347022

Immunocytochemistry of HeLa cells using anti-PPP1A mouse mAb diluted 1:50.

TA347022

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
TA327322	PPP1CA antibody
	Rabbit anti-PPP1CA Polyclonal Antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt		0.1 mg / €335.00
							OriGene Technologies, Inc.
+3 additional images	Highly validated antibody!
TA808819	PPP1CA antibody
	PPP1CA mouse monoclonal antibody, clone OTI6E5 (formerly 6E5)
	Mouse	IgG1	OTI6E5	Purified	Hu	P, WB	0.1 ml / €360.00
							OriGene Technologies, Inc.
+8 additional images	Highly validated antibody!
AM03118PU-N	PPP1CA antibody
	Mouse	IgG2a	4G3	Purified	Hu	E, ICC/IF, P, WB	0.1 ml / €330.00
							OriGene Technologies GmbH
+3 additional images	Highly validated antibody!
AM03118PU-S	PPP1CA antibody
	Mouse	IgG2a	4G3	Purified	Hu	E, ICC/IF, P, WB	50 µl / €220.00
							OriGene Technologies GmbH
+3 additional images	Highly validated antibody!
TA323467	PPP1CA antibody
	Anti-PPP1CA Rabbit Polyclonal Antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt	WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
TA347021	PPP1CA antibody
	Mouse Monoclonal PPP1A Antibody
	Mouse	IgG1	1C11-C10-H5-E9	Purified		ICC/IF, WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
+2 additional images
TA347022	PPP1CA antibody
	Mouse Monoclonal PPP1A Antibody
	Mouse	IgG1	1H1-F7-D8	Purified		ICC/IF, WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
+1 additional image
AM06514SU-N	PPP1CA antibody
	Mouse	IgG1	6D1	Ascites	Hu, Ms	E, WB	0.1 ml / €340.00
							OriGene Technologies GmbH
AM06712SU-N	PPP1CA antibody
	Mouse	IgG1	5E9	Ascites	Hu	E, WB	0.1 ml / €410.00
							OriGene Technologies GmbH
+2 additional images
AM20663PU-N	PPP1CA antibody
	Mouse	IgG2b	PP-1A	Purified	Hu, Ms, Rb, Rt	ICC/IF, WB	0.1 mg / €390.00
							OriGene Technologies GmbH
AP05032PU-N	PPP1CA antibody
	Rabbit	IgG		Purified	Bov, Hu, Ms, Rt	IP, WB	0.1 mg / €400.00
							OriGene Technologies GmbH
AP05033PU-N	PPP1CA antibody
	Sheep	IgG		Purified	Bov, Hu, Ms, Rt	IP, WB	0.1 mg / €400.00
							OriGene Technologies GmbH
AP20315PU-N	PPP1CA antibody
	Rabbit			Aff - Purified	Hu, Ms, Rt	P, WB	0.1 mg / €370.00
							OriGene Technologies GmbH
AP55265SU-N	PPP1CA antibody
	Rabbit			Serum	Hu	C, E, WB	0.2 ml / €430.00
							OriGene Technologies GmbH

Proteins & Growth Factors

Catalog No.	Species	Purity	Source
TP760246	PPP1CA (full length, N-term HIS tag, transcript variant 3)
	Recombinant protein of human protein phosphatase 1, catalytic subunit, alpha isozyme (PPP1CA), transcript variant 3, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug
	Human	> 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	E. coli	50 µg / €205.00
				OriGene Technologies, Inc.
TP760285	PPP1CA (full length, N-term HIS tag, transcript variant 1)
	Recombinant protein of human protein phosphatase 1, catalytic subunit, alpha isozyme (PPP1CA), transcript variant 1, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug
	Human	> 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	E. coli	50 µg / €205.00
				OriGene Technologies, Inc.

Lysates

Catalog No.
LY400954	PPP1CA overexpression lysate
		0.1 mg / €295.00
		OriGene Technologies, Inc.
LY423356	PPP1CA overexpression lysate
		0.1 mg / €295.00
		OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.
MR204856L2V	Lenti ORF particles, Ppp1ca (GFP-tagged) - Mouse protein phosphatase 1, catalytic subunit, alpha isoform (Ppp1ca), 200 uL, >10^7 TU/mL
		200 µl / €930.00
		OriGene Technologies, Inc.

MR204856L1V	Lenti ORF particles, Ppp1ca (Myc-DDK-tagged) - Mouse protein phosphatase 1, catalytic subunit, alpha isoform (Ppp1ca), 200 uL, >10^7 TU/mL
		200 µl / €830.00
		OriGene Technologies, Inc.

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