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PPP1CA antibody

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Principal name

PPP1CA antibody

Alternative names for PPP1CA antibody

PPP1A, PP-1A, Serine/threonine-protein phosphatase PP1-alpha catalytic subunit

SwissProt ID

P62136 (Human), P62137 (Mouse), P62138 (Rat), P62139 (Rabit), Q3T0E7 (Bovin), Q8WMS6 (Canfa)

Gene ID

5499 (PPP1CA), 100009298, 19045 (Ppp1ca), 24668

Ncbi ID

9606, NP_001008709.1, NP_002699, NP_001095176, NP_001008709, NM_002708, NM_001008709, NP_114074.1, NM_031868, NM_031527, NM_206873

Available reactivities

Hu (Human), Bov (Bovine), Ms (Mouse), Rt (Rat), Can (Canine), Por (Porcine), Rb (Rabbit), Eq (Equine), GP (Guinea Pig)

Available hosts

Mouse, Rabbit, Sheep

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Frozen Sections (C)

Background of PPP1CA antibody

PPP1CA is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory.The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene.

General readings

1. Barker, H. M.; Jones, T. A.; da Cruz e Silva, E. F.; Spurr, N. K.; Sheer, D.; Cohen, P. T. W.: Localization of the gene encoding a type I protein phosphatase catalytic subunit to human chromosome band 11q13. Genomics 7: 159-166, 1990.
2 Genoux, D.; Haditsch, U.; Knobloch, M.; Michalon, A.; Storm, D.; Mansuy, I. M. : Protein phosphatase 1 is a molecular constraint on learning and memory. Nature 418: 970-975, 2002.

H00005499-A01 - PPP1CA
PPP1CA polyclonal antibody (A01), Lot # 060102JCO1 Western Blot analysis of PPP1CA expression in HL-60 ( Cat # L014V1 ).
AM03118PU-N - PPP1CA
Immunohistochemistry: AM03118PU-N PPP1CA antibody staining of Formalin-Fixed, Paraffin-Embedded Human Brain, Cortex at 5 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
AM03118PU-N - PPP1CA
Immunohistochemistry: AM03118PU-N PPP1CA antibody staining of Formalin-Fixed, Paraffin-Embedded Human Adrenal at 5 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
AM03118PU-N - PPP1CA
Western blot analysisCell lysates of HepG2 (45 µg) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PPP1CA (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
AM03118PU-N - PPP1CA
Cell lysates of HepG2 (45ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PPP1CA (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
AP05032PU-N - PPP1CA
PP1 alpha antibody western blot of total rat brain homogenate. (+) indicates staining done in the presence of specific blocking peptide or (-) Peptide Control.
MAB1112 - PPP1CA
Western blot analysis of cell lysates of HepG2 ( 45 µg ) were resolved by SDS - PAGE , transferred to NC membrane and probed with PPP1CA monoclonal antibody , clone 4G3 ( 1 : 1000 ) ( Cat # MAB1112 ) . Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.
PAB10410 - PPP1CA
Western blot of PPP1CA polyclonal antibody ( Cat # PAB10410 ) from total rat brain homogenate. (+) indicates staining done in the presence of specific blocking peptide or (-) peptide control.
MAB2728 - PPP1CA
Immunoblot analysis of cell lysates.Lane 1 : Jurkat cell lysate. Lnae 2 : MOLT 4 cell lysate.Lane 3 : HT-29 cell lysate.Lane 4 : A-431 cell lysate.Lane 5 : SK-N-SH cell lysate.Lane 6 : PC-12 cell lysate.Lane 7 : NIH/3T3 cell lysate.
ARP58652_P050 - PPP1CA
HEK293, H1299; WB Suggested Anti-PPP1CA Antibody . . Positive Control: Lane 1: 40ug HEK293 lysate Lane 2: 40ug H1299 lysate . Primary Antibody Dilution : 1:1000. Secondary Antibody : Goat anti-rabbit-HRP . Secondry Antibody Dilution : 1:5000. Submitted by: Jose Luis Rosa, Universitat de Barcelona; PPP1CA antibody - N-terminal region (ARP58652_P050) in HEK293, H1299 cells using Western Blot
ARP58652_P050 - PPP1CA
Human Jurkat; WB Suggested Anti-PPP1CA Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Jurkat cell lysate. PPP1CA is supported by BioGPS gene expression data to be expressed in Jurkat.; PPP1CA antibody - N-terminal region (ARP58652_P050) in Human Jurkat cells using Western Blot
AP20315PU-N - PPP1CA
Immunohistochemistry analyzes of PP1α antibody (Cat.-No.: AP20315PU-N) in paraffin-embedded human breast carcinoma tissue.
A0726-1 - PPP1CA
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PP1-alpha (Phospho-Thr320) Antibody. The picture on the right is treated with the synthesized peptide.
B0726-1 - PPP1CA
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using PP1-alpha (Ab-320) Antibody. The picture on the right is treated with the synthesized peptide.
B0726-1 - PPP1CA
Western blot analysis of extracts from Jurkat cells, using PP1-alpha (Ab-320) Antibody. The lane on the right is treated with the synthesized peptide.
AR39114PU-N - PPP1CA
AR39114PU-N - PPP1CA
PPP1CA, 1-330 aa, Human, His-tagged
AM06514SU-N - PPP1CA
Western blot analysis using PPP1A antibody Cat.-No AM06514SU-N against Hela (1) and NIH/3T3 (2) cell lysate.
AM06712SU-N - PPP1CA
Western blot analysis using PPP1CA mouse mAb against Hela (1), HepG2 (2), MCF-7 (3), Jurkat (4) and A549 (5) cell lysate.
AM06712SU-N - PPP1CA
Western blot analysis using PPP1CA mAb against human PPP1CA (AA: 174-330) recombinant protein. (Expected MW is 38 kDa)
AM06712SU-N - PPP1CA
Red: Control Antigen (100ng)Purple: Antigen (10ng)Green: Antigen (50ng)Blue: Antigen (100ng)
TA808819 - PPP1CA
Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-PPP1CA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808819)(1:150)
TA808819 - PPP1CA
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PPP1CA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808819)(1:150)
TA808819 - PPP1CA
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-PPP1CA mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808819)(1:150)
TA808819 - PPP1CA
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-PPP1CA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808819)(1:150)
TA808819 - PPP1CA
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PPP1CA mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808819)(1:150)
TA808819 - PPP1CA
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PPP1CA mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808819)(1:150)
TA808819 - PPP1CA
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PPP1CA mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808819)(1:150)
TA808819 - PPP1CA
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PPP1CA mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808819)(1:150)
TA808819 - PPP1CA
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPP1CA (RC212268, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPP1CA.(1:2000)
TP760246 - PPP1CA
TP760285 - PPP1CA
TA323467 - PPP1CA
Predicted band size: 38 kDa . Positive control: HeLa cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
TA327322 - PPP1CA
Western blot analysis of extracts of various cell lines, using PPP1CA antibody.
TA327322 - PPP1CA
Immunohistochemistry of paraffin-embedded human kidney using PPP1CA antibody at dilution of 1:200 (400x lens).
TA327322 - PPP1CA
Immunohistochemistry of paraffin-embedded human stomach cancer using PPP1CA antibody at dilution of 1:200 (400x lens).
TA327322 - PPP1CA
Immunofluorescence analysis of U2OS cell using PPP1CA antibody. Blue: DAPI for nuclear staining.
TA343228 - PPP1CA
Host: Rabbit; Target Name: Ppp1ca; Sample Tissue: Mouse Spleen lysates; Antibody Dilution: 1.0 ug/ml
TA344247 - PPP1CA
WB Suggested Anti-PPP1CA Antibody ; Positive Control: Lane 1: 40ug HEK293 lysate Lane 2: 40ug H1299 lysate; Primary Antibody Dilution : 1:1000; Secondary Antibody : Goat anti-rabbit-HRP; Secondry Antibody Dilution : 1:5000; Submitted by: Jose Luis Rosa, U
TA344247 - PPP1CA
WB Suggested Anti-PPP1CA Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Jurkat cell lysatePPP1CA is supported by BioGPS gene expression data to be expressed in Jurkat
TA347021 - PPP1CA
Western blot detection of PPP1A in MCF7,K562,Hela,HEPG2 and Jurkat whole cell lysates using PPP1A mouse mAb (1:1000 diluted).Predicted band size: 38KDa.Observed band size: 38KDa.
TA347021 - PPP1CA
IHC of paraffin-embedded human breast cancer using anti-PPP1A mouse mAb diluted 1/500-1/1000.
TA347021 - PPP1CA
Immunocytochemistry stain of Hela using PPP1A mouse mAb (1:300).
TA347022 - PPP1CA
Western blot detection of PPP1A in HepG2,MCF7 and Hela cell lysates using PPP1A mouse mAb (1:1000 diluted).Predicted band size:37KDa.Observed band size:37KDa.
TA347022 - PPP1CA
Immunocytochemistry of HeLa cells using anti-PPP1A mouse mAb diluted 1:50.
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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PPP1CA antibody
  Rabbit anti-PPP1CA Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using PPP1CA antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €335.00
  OriGene Technologies, Inc.
+3 additional images

Highly validated antibody!

PPP1CA antibody
  Anti-PPP1CA Rabbit Polyclonal Antibody  
Predicted band size: 38 kDa . Positive control: HeLa cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

PPP1CA antibody
PP1 alpha antibody western blot of total rat brain homogenate. (+) indicates staining done in the presence of specific blocking peptide or (-) Peptide Control. Rabbit IgG Purified Bov, Hu, Ms, Rt IP, WB
0.1 mg / €400.00
  OriGene Technologies GmbH

PPP1CA antibody
Immunohistochemistry analyzes of PP1α antibody (Cat.-No.: AP20315PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €370.00
  OriGene Technologies GmbH

PPP1CA antibody
PPP1CA Rabbit Serum Hu C, E, WB
0.2 ml / €430.00
  OriGene Technologies GmbH

5 Item(s)

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  1. Primary Antibody (5)
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