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PPP1R13B antibody

Principal name

PPP1R13B antibody

Alternative names for PPP1R13B antibody

ASPP1, KIAA0771, Apoptosis-stimulating of p53 protein 1, Protein phosphatase 1 regulatory subunit 13B

SwissProt ID

Q62415 (Mouse), Q96KQ4 (Human)

Gene ID

23368 (PPP1R13B), 21981 (Ppp1r13b)

Ncbi ID

9606, NP_056131, NP_056131.2, NP_035755.1, NM_015316

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Rt (Rat), Ze (Zebrafish), African clawed frog, Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse, Sheep

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of PPP1R13B antibody

PPP1R13B is a member of the ASPP (apoptosis-stimulating protein of p53) family of p53 interacting proteins. The protein contains four ankyrin repeats and an SH3 domain involved in protein-protein interactions. ASPP proteins are required for the induction of apoptosis by p53-family proteins. They promote DNA binding and transactivation of p53-family proteins on the promoters of proapoptotic genes. Expression of this gene is regulated by the E2F transcription factor.This gene encodes a member of the ASPP (apoptosis-stimulating protein of p53) family of p53 interacting proteins. The protein contains four ankyrin repeats and an SH3 domain involved in protein-protein interactions. ASPP proteins are required for the induction of apoptosis by p53-family proteins. They promote DNA binding and transactivation of p53-family proteins on the promoters of proapoptotic genes. Expression of this gene is regulated by the E2F transcription factor.

General readings

Agirre,X., (2006) Oncogene 25 (13), 1862-1870

Western blot using PPP1R13B polyclonal antibody ( Cat # PAB9974 ) to detect over-expressed PPP1R13B in MCF-7 cells ( lane 2, arrowhead ).Lane 1 is a non-transfected control.Lane 3 is MCF-7 cells over-expressing TP53BP2.Cell extracts were electrophoresed and transferred to nitrocellulose.The membrane was probed with the primary antibody at a 1 : 1,000 dilution.The identity of the lower MW band at approximately 50 kDa is unknown.Primary experimental data indicate that the unknown band intensifies in extracts from p53 siRNA knockdown cells.Personal Communication, H. Yang, Univ. Oklahoma, Oklahoma City, OK.

PAB9974

Human HepG2; WB Suggested Anti-PPP1R13B Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate. PPP1R13B is supported by BioGPS gene expression data to be expressed in HepG2.; PPP1R13B antibody - middle region (ARP47979_P050) in Human HepG2 cells using Western Blot

ARP47979_P050

Western blot analysis of PPP1R13B expression in HEK293T (A); Raw264.7 (B); PC12 (C) whole cell lysates.

TP321172

WB using Anti-ASPP1 to detect over-expressed ASPP1 in MCF-7 cells (lane 2, arrowhead). Lane 1 is a non-transfected control. Lane 3 is MCF-7 cells over-expressing ASPP2. Cell extracts were electrophoresed and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:1,000 dilution. The identity of the lower MW band at approximately 50kDa is unknown. Primary experimental data indicate that the unknown band intensifies in extracts from p53 siRNA knockdown cells.

TA319477

Predicted cell location: Cytoplasm . Positive control: Human liver cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using ASPP1 antibody at dilution 1/35, on the right is treated with the synthetic peptide. (Original magnification: x200)

TA322354

WB Suggested Anti-PPP1R13B Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: HepG2 cell lysate;PPP1R13B is supported by BioGPS gene expression data to be expressed in HepG2

TA342553

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
TA319477	PPP1R13B antibody
	Rabbit polyclonal anti-ASPP1 antibody
	Rabbit	IgG	Aff - Purified	Hu, Ms	E, WB	0.1 mg / €515.00
						OriGene Technologies, Inc.
TA322354	PPP1R13B antibody
	Anti-ASPP1 Rabbit Polyclonal Antibody
	Rabbit	IgG	Purified	Hu, Ms, Rt	E, P	0.1 ml / €335.00
						OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No.	Species	Purity	Source
TP321172	PPP1R13B
	Recombinant protein of human protein phosphatase 1, regulatory (inhibitor) subunit 13B (PPP1R13B)
	Human	> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €788.00
				OriGene Technologies, Inc.

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