PRKACB antibody
Principal name
PRKACB antibody
Alternative names for PRKACB antibody
PKA C-beta, PKACb, PKA catalytic subunit beta, cAMP-dependent protein kinase catalytic subunit beta
SwissProt ID
P05131 (Bovin), P05383 (Pig), P22694 (Human), P68181 (Mouse), P68182 (Rat), Q5BNF3 (Human)
Ncbi ID
9606, NP_002722.1, NP_002722, NP_891993, NM_002731, NM_207578, NM_001164200, NM_001164199, NM_182948
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)
Background of PRKACB antibody
- Kit Component:
- - KN202131G1, PRKACB gRNA vector 1 in pCas-Guide vector
- KN202131G2, PRKACB gRNA vector 2 in pCas-Guide vector
- KN202131D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Wu K.-J., et al. (2002) Oncogene. 21:7872-7882Lignitto L., et al. (2011) Nat. Cell Biol. 13:412-422
Detection limit for recombinant GST tagged PRKACB is approximately 1ng/ml as a capture antibody.
Figure 1. Staining PRKACB in Kidney by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue.
The PRKACB polyclonal antibody ( Cat # PAB4578 ) is used in Western blot to detect PRKACB in A-375 cell lysate .
The anti-PRKACB Pab is used in Western blot to detect PRKACB in A375 cell lysate.
Human Lung; WB Suggested Anti-PRKACB Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Lung; PRKACB antibody - middle region (ARP56416_P050) in Human Lung cells using Western Blot
Human HCT15; WB Suggested Anti-PRKACB Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: HCT15 cell lysate; PRKACB antibody - N-terminal region (ARP56415_P050) in Human HCT15 cells using Western Blot
Immunohistochemical analysis of PKA C beta staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of PKA C beta expression in HEK293T (A); NIH3T3 (B); mouse lung (C); rat lung (D) whole cell lysates.
Western blot analysis of extracts from Jurkat cells and COLO205 cells, using KAPCB antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts of SW480 cell line, using PRKACB antibody.
Immunofluorescence analysis of U2OS cell using PRKACB antibody. Blue: DAPI for nuclear staining.
WB Suggested Anti-PRKACB Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: HCT15 cell lysate
WB Suggested Anti-PRKACB Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human Lung
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA314382 | PRKACB antibody |
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| Rabbit polyclonal anti-KAPCB antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA332611 | PRKACB antibody |
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| Rabbit anti-PRKACB Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
|
||
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| AP07931PU-N | PRKACB pThr198 (193-202) antibody |
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 |
Rabbit | Purified | Hu | E, P |
50 µg /
€425.00
|
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| OriGene Technologies GmbH | |||||||
| AP13600PU-N | PRKACB (N-term) antibody |
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 |
Rabbit | Ig | Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP307218 | PRKACB (transcript variant 2) |
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| Recombinant protein of human protein kinase, cAMP-dependent, catalytic, beta (PRKACB), transcript variant 2 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY403923 | PRKACB overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR219172L2V | Lenti ORF particles, Prkacb (GFP-tagged) - Mouse protein kinase, cAMP dependent, catalytic, beta (Prkacb), transcript variant 3, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR217190L2V | Lenti ORF particles, Prkacb (GFP-tagged) - Mouse protein kinase, cAMP dependent, catalytic, beta (Prkacb), transcript variant 4, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR219172L1V | Lenti ORF particles, Prkacb (Myc-DDK-tagged) - Mouse protein kinase, cAMP dependent, catalytic, beta (Prkacb), transcript variant 3, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
| MR217190L1V | Lenti ORF particles, Prkacb (Myc-DDK-tagged) - Mouse protein kinase, cAMP dependent, catalytic, beta (Prkacb), transcript variant 4, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||