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PRKACB antibody

Principal name

PRKACB antibody

Alternative names for PRKACB antibody

PKA C-beta, PKACb, PKA catalytic subunit beta, cAMP-dependent protein kinase catalytic subunit beta

SwissProt ID

P05131 (Bovin), P05383 (Pig), P22694 (Human), P68181 (Mouse), P68182 (Rat), Q5BNF3 (Human)

Gene ID

5567 (PRKACB), 18749 (Prkacb)

Ncbi ID

9606, NP_002722.1, NP_002722, NP_891993, NM_002731, NM_207578, NM_001164200, NM_001164199, NM_182948

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), Por (Porcine), Eq (Equine), GP (Guinea Pig), Can (Canine), Ze (Zebrafish)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of PRKACB antibody

Kit Component:: - KN202131G1, PRKACB gRNA vector 1 in pCas-Guide vector
- KN202131G2, PRKACB gRNA vector 2 in pCas-Guide vector
- KN202131D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:: CRISPR/CAS9 Genome Editing Manual

General readings

Wu K.-J., et al. (2002) Oncogene. 21:7872-7882Lignitto L., et al. (2011) Nat. Cell Biol. 13:412-422

Detection limit for recombinant GST tagged PRKACB is approximately 1ng/ml as a capture antibody.

H00005567-M01

Figure 1. Staining PRKACB in Kidney by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue.

AP07931PU-N

The PRKACB polyclonal antibody ( Cat # PAB4578 ) is used in Western blot to detect PRKACB in A-375 cell lysate .

PAB4578

The anti-PRKACB Pab is used in Western blot to detect PRKACB in A375 cell lysate.

AP13600PU-N

Human Lung; WB Suggested Anti-PRKACB Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Lung; PRKACB antibody - middle region (ARP56416_P050) in Human Lung cells using Western Blot

ARP56416_P050

Western blot analysis of extracts from Jurkat/COLO205 cells, using KAPCB Antibody. The lane on the right is treated with the synthesized peptide.

Human HCT15; WB Suggested Anti-PRKACB Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: HCT15 cell lysate; PRKACB antibody - N-terminal region (ARP56415_P050) in Human HCT15 cells using Western Blot

ARP56415_P050

Immunohistochemical analysis of PKA C beta staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Western blot analysis of PKA C beta expression in HEK293T (A); NIH3T3 (B); mouse lung (C); rat lung (D) whole cell lysates.

TP307218

Western blot analysis of extracts from Jurkat cells and COLO205 cells, using KAPCB antibody.The lane on the right is treated with the synthesized peptide.