PRKACG antibody
Principal name
PRKACG antibody
Alternative names for PRKACG antibody
PKA C-gamma, PKACg, KAPG, PKA catalytic subunit gamma, cAMP-dependent protein kinase catalytic subunit gamma
SwissProt ID
P22612 (Human)
Gene ID
5568 (PRKACG)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P)
Background of PRKACG antibody
cAMP-dependent protein kise catalytic subunit gamma is an enzyme that in humans is encoded by the PRKACG gene. Cyclic AMP-dependent protein kise (PKA) consists of two catalytic subunits and a regulatory subunit dimer. This gene encodes the gamma form of its catalytic subunit. The gene is intronless and is thought to be a retrotransposon derived from the gene for the alpha form of the PKA catalytic subunit. PRKACG has been shown to interact with Ryanodine receptor 2.
Western Blot analysis of PRKACG expression in transfected 293T cell line (H00005568-T01) by PRKACG MaxPab polyclonal antibody.Lane 1: PRKACG transfected lysate(38.61 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of PRKACG expression in transfected 293T cell line (H00005568-T02) by PRKACG MaxPab polyclonal antibody.Lane 1: PRKACG transfected lysate(40.40 KDa).Lane 2: Non-transfected lysate.
Immunohistochemical analysis of paraffin-embedded liver cancer tissues using PRKACG antibody AM06770SU-N with DAB staining.
Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PRKACG antibody AM06770SU-N with DAB staining.
Western blot analysis using PRKACG mAb against human PRKACG antibody AM06770SU-N (AA: 164-351) recombinant protein (Expected MW is 47.1 kDa)
Flow Cytometric analysis of MCF-7 cells using PRKACG antibody AM06770SU-N (green) and negative control (red).
Immunohistochemical analysis of PKA C gamma staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of PKA C gamma expression in HeLa (A); MCF7 (B) whole cell lysates.
Western blot analysis of extracts from COLO205 cells, using KAPCG antibody.The lane on the right is treated with the synthesized peptide.
Predicted cell location: Cytoplasm . Positive control: Human thyroid and lung cancer tissue . Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human thyroid cancer tissue using PRKACG antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification:x 200)
Predicted cell location: Cytoplasm . Positive control: Human thyroid and lung cancer tissue . Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human lung cancer tissue using PRKACG antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification:x200)
Predicted cell location: Cytoplasm . Positive control: Human lung and liver cancer tissue . Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human lung cancer tissue using PRKACG antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification:x 200)
Predicted cell location: Cytoplasm . Positive control: Human lung and liver cancer tissue . Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human liver cancere tissue using PRKACG antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification:x200)
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- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AM06770SU-N | PRKACG antibody |
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Mouse | IgG1 | 2E4 | Ascites | Hu | E, P, WB |
0.1 ml /
€410.00
|
| OriGene Technologies GmbH | |||||||
 +4 additional images |
Highly validated antibody! |
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