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PTCH1 antibody

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Principal name

PTCH1 antibody

Alternative names for PTCH1 antibody

PTCH, PTC1, PTC, Patched 1, Protein patched homolog 1

SwissProt ID

O42335 (Cynpy), Q09614 (Caeel), Q13635 (Human), Q61115 (Mouse), Q90693 (Chick), Q98864 (Danre)

Gene ID

5727 (PTCH1), 30181 (ptch2), 19206 (Ptch1)

Ncbi ID

NP_000255.2, 9606, 7955, AAR21240, NP_000255, NP_032983.1, NM_008957, NM_001083607, NM_000264, NM_001083603

Available reactivities

Chk (Chicken), Hu (Human), Ms (Mouse), Rt (Rat), Ze (Zebrafish), Can (Canine), Bt (Bat), Bov (Bovine), Eq (Equine), Hst (Hamster), Mky (Monkey), Op (Opossum), Prim (Primate), Rb (Rabbit), L (Lizard), Bear, Por (Porcine), GP (Guinea Pig)

Available hosts

Rabbit, Mouse, Rat, Goat

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of PTCH1 antibody

PTCH (Patched protein homolog 1) is a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). PTCH associates with the smoothened protein (SMO) to transduce the hedgehog's proteins signal.PTCH has a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis. PTCH is expressed in the adult brain, lung, liver, heart, placenta, skeletal muscle, pancreas and kidney. It is also expressed in tumor cells but not in normal skin. During development PTCH is found in all major target tissues of sonic hedgehog, such as the ventral neural tube, somites, and tissues surrounding the zone of polarizing activity of the limb bud.
Defects in PTCH are probably the cause of basal cell nevus syndrome also known as Gorlin syndrome or Gorlin-Goltz syndrome.

General readings

1. Ju B, Spitsbergen J, Eden CJ, Taylor MR, Chen W.
Co-activation of hedgehog and AKT pathways promote tumorigenesis in zebrafish. Mol Cancer. 2009 Jun 25;8:40.
PMID: 19555497

H00005727-M02 - PTCH1
Detection limit for recombinant GST tagged PTCH is approximately 0.03ng/ml as a capture antibody.
H00005727-M03 - PTCH1
Detection limit for recombinant GST tagged PTCH is approximately 0.1ng/ml as a capture antibody.
H00005727-M04 - PTCH1
Detection limit for recombinant GST tagged PTCH is approximately 1ng/ml as a capture antibody.
H00005727-M05 - PTCH1
Detection limit for recombinant GST tagged PTCH is approximately 0.3ng/ml as a capture antibody.
H00005727-M06 - PTCH1
Detection limit for recombinant GST tagged PTCH is approximately 1ng/ml as a capture antibody.
H00005727-M07 - PTCH1
Detection limit for recombinant GST tagged PTCH is approximately 0.3ng/ml as a capture antibody.
H00005727-M08 - PTCH1
Detection limit for recombinant GST tagged PTCH is approximately 1ng/ml as a capture antibody.
AP06278PU-N - PTCH1
Immunohistochemistry (IHC) analysis of Patched antibody (Cat.-No.: AP06278PU-N) in paraffin-embedded human brain tissue.
AP06278PU-N - PTCH1
Western blot (WB) analysis of Patched antibody (Cat.-No.: AP06278PU-N) at 1/500 dilution
Lane1:Hela whole cell lysate
Lane2:NIH-3T3 whole cell lysate
Lane3:PC12 whole cell lysate
Lane4:MCF-7 whole cell lysate
PAB7063 - PTCH1
PTCH1 polyclonal antibody ( Cat # PAB7063 ) ( 1 µg/ml ) staining of human brain lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
H00005727-M10 - PTCH1
PTCH monoclonal antibody (M10), clone 3B3 Western Blot analysis of PTCH expression in Y-79 ( Cat # L042V1 ).
ARP44249_P050 - PTCH1
Human Kidney; Kidney; PTCH1 antibody - C-terminal region (ARP44249_P050) in Human Kidney cells using Immunohistochemistry
ARP44249_P050 - PTCH1
Human Jurkat; WB Suggested Anti-PTCH1 Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysate; PTCH1 antibody - C-terminal region (ARP44249_P050) in Human Jurkat cells using Western Blot
PAB12543 - PTCH1
Western blot analysis of human PTCH1 in fetal brain lysate with PTCH1 polyclonal antibody ( Cat # PAB12543 ) at a 1 : 500 dilution.
C0296-1 - PTCH1
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Patched Antibody. The picture on the right is treated with the synthesized peptide.
C0296-1 - PTCH1
Western blot analysis of extracts from mouse muscle cells, using Patched Antibody. The lane on the right is treated with the synthesized peptide.
AP22448PU-N - PTCH1
AP22448PU-N PTCH1 antibody staining of Paraffin Embedded Human Colon at 3.8 µg/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
AP23037PU-N - PTCH1
Immunohistochemistry Image: Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
AP23037PU-N - PTCH1
Immunohistochemistry Image: Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)
AP31827PU-N - PTCH1
Formalin-Fixed, Paraffin-Embedded Human Small Intestine stained with PTCH1 antibody Cat.-No AP31827PU-N
AP31827PU-N - PTCH1
Western blot analysis of extracts from mouse muscle cells using PTCH1 antibody Cat.-No AP31827PU-N. The lane on the right is treated with the synthesized peptide.
AP32805PU-N - PTCH1
Staining of Mouse fetal Kidney lysate using PTCH1 Antibody Cat.-No AP32805PU-N at 2 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
CPA1964-100ul - PTCH1
Western blot analysis of Patched expression in HeLa (A); Ramos (B); MCF7 (C); NIH3T3 (D); PC12 (E) whole cell lysates.
TA500122 - PTCH1
Anti-Patched1 mouse monoclonal antibody (TA500122) immunofluorescent staining of HeLa cells transiently transfected by pCMV6-ENTRY PTCH1(RC201597)
TA500122 - PTCH1
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTCH1(RC216999) (RC216999, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTCH1.
TA500123 - PTCH1
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PTCH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500123)
TA500123 - PTCH1
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PTCH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500123)
TA500123 - PTCH1
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PTCH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500123)
TA500123 - PTCH1
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PTCH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500123)
TA500123 - PTCH1
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PTCH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500123)
TA500123 - PTCH1
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PTCH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500123)
TA500123 - PTCH1
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PTCH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500123)
TA500123 - PTCH1
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PTCH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500123)
TA500123 - PTCH1
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PTCH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500123)
TA500123 - PTCH1
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-PTCH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500123)
TA500123 - PTCH1
TA500123 - PTCH1
Anti-PTCH1 mouse monoclonal antibody (TA500123) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PTCH1(RC216999).
TA500123 - PTCH1
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTCH1 (RC216999, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTCH1.
TA590748 - PTCH1
Immunohistochemistry: Patched 1 Antibody - With exception of malignant gliomas, malignant tissues such as in colo-rectal cancer showed in general a moderate to strong cytoplasmic and membranous positivity. Image was provided courtesy of the Swedish Human Proteome Resource (HPR) Center.
TA590748 - PTCH1
Immunohistochemistry: Patched 1 Antibody - Normal and malignant tissues showed in general a moderate to strong cytoplasmic and membranous positivity. Malignant gliomas were mostly weakly stained or negative. Stroma and endothelial lining were distinctly stained in normal and tumor tissues. Image and statement courtesy of the Human Protein Atlas (HPA).
TA590748 - PTCH1
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
TA590748 - PTCH1
TA590748 - PTCH1
Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17; Lane 2: RT-4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: TonsilThis validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
TA590748 - PTCH1
ELISA: Patched 1 Antibody
TA803741 - PTCH1
Human recombinant protein fragment corresponding to amino acids 770-1027 of human PTCH1 (NP_000255) produced in E.coli.
TA803788 - PTCH1
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTCH1 (RC216999, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTCH1.
TA803822 - PTCH1
Human recombinant protein fragment corresponding to amino acids 770-1027 of human PTCH1 (NP_000255) produced in E.coli.
TA803949 - PTCH1
Human recombinant protein fragment corresponding to amino acids 770-1027 of human PTCH1 (NP_000255) produced in E.coli.
TP710156 - PTCH1
TA303124 - PTCH1
TA303124 (1µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA309821 - PTCH1
Western Blot: Patched 1 Antibody -Analysis of Human PTCH1 in Fetal brain Lysate with anti huPTCH1 at a 1:500 dilution.
TA334665 - PTCH1
WB Suggested Anti-PTCH1 Antibody Titration: 0.2-1 ug/ml; Positive Control: Jurkat cell lysate
TA334665 - PTCH1
Kidney
TA347455 - PTCH1
Western blot analysis on mouse muscle cell lysate using Patched Antibody
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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PTCH1 antibody
  Patched1 (Patched) mouse monoclonal antibody, clone OTI5C7 (formerly 5C7)  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PTCH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500123) Mouse IgG2b OTI5C7 Purified Hu ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.
+12 additional images

Top Selling product!

Highly validated antibody!

TrueMAB

PTCH1 (C-term) antibody
AP22448PU-N PTCH1 antibody staining of Paraffin Embedded Human Colon at 3.8 µg/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat Aff - Purified Hu, Ms, Rt E, ICC/IF, P
0.1 mg / €380.00
  OriGene Technologies GmbH
+3 additional images

Highly validated antibody!

PTCH1 (266-280) antibody
Staining of Mouse fetal Kidney lysate using PTCH1 Antibody Cat.-No AP32805PU-N at 2 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €380.00
  OriGene Technologies GmbH
+1 additional image

OriGene recommends this product for Western blotting.

PTCH1 antibody
  Goat Polyclonal Antibody against PTCH  
TA303124 (1µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por E, ICC/IF, WB
0.1 mg / €360.00
  OriGene Technologies, Inc.

PTCH1 antibody
  Rabbit Polyclonal Patched 1 Antibody  
Western Blot: Patched 1 Antibody -Analysis of Human PTCH1 in Fetal brain Lysate with anti huPTCH1 at a 1:500 dilution. Rabbit Purified Chk, Hu, Ms, Rt C, E, ICC/IF, WB
0.1 ml / €395.00
  OriGene Technologies, Inc.

PTCH1 antibody
  Rabbit Polyclonal Anti-Patched Antibody  
Western blot analysis on mouse muscle cell lysate using Patched Antibody Rabbit IgG Purified Hu, Ms WB
0.1 mg / €335.00
  OriGene Technologies, Inc.

PTCH1 antibody
  anti-Patched1 (Patched) mouse monoclonal antibody, clone OTI3c2 (formerly 3c2)  
Anti-Patched1 mouse monoclonal antibody (TA500122) immunofluorescent staining of HeLa cells transiently transfected by pCMV6-ENTRY PTCH1(RC201597) Mouse IgG2b OTI3c2 Purified Hu ICC/IF, WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
+1 additional image

TrueMAB

PTCH1 antibody
  PTCH1 mouse monoclonal antibody, clone OTI1H10 (formerly 1H10)  
Human recombinant protein fragment corresponding to amino acids 770-1027 of human PTCH1 (NP_000255) produced in E.coli. Mouse IgG1 OTI1H10 Purified Hu WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
 

TrueMAB

PTCH1 antibody
  PTCH1 mouse monoclonal antibody, clone OTI1H8 (formerly 1H8)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTCH1 (RC216999, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTCH1. Mouse IgG1 OTI1H8 Purified Hu WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
 

TrueMAB

PTCH1 antibody
  PTCH1 mouse monoclonal antibody, clone OTI5G9 (formerly 5G9)  
Human recombinant protein fragment corresponding to amino acids 770-1027 of human PTCH1 (NP_000255) produced in E.coli. Mouse IgG1 OTI5G9 Purified Hu WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
 

TrueMAB

PTCH1 antibody
  PTCH1 mouse monoclonal antibody, clone OTI6C10 (formerly 6C10)  
Human recombinant protein fragment corresponding to amino acids 770-1027 of human PTCH1 (NP_000255) produced in E.coli. Mouse IgG1 OTI6C10 Purified Hu WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
 

TrueMAB

PTCH1 antibody
Immunohistochemistry (IHC) analysis of Patched antibody (Cat.-No.: AP06278PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €370.00
  OriGene Technologies GmbH
+1 additional image

PTCH1 antibody
PTCH1 Goat Aff - Purified Ze E, ICC/IF
0.1 mg / €380.00
  OriGene Technologies GmbH

PTCH1 (1431-1442) antibody
Immunohistochemistry Image: Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat Aff - Purified Bt, Can, Elephant, Eq, Hu, Mky E, P
50 µg / €425.00
  OriGene Technologies GmbH
+1 additional image

PTCH1 antibody
PTCH1 Rabbit Serum Ms C, E, WB
0.2 ml / €430.00
  OriGene Technologies GmbH

PTCH1 antibody
PTCH1 Rabbit Serum Hu C, E, WB
0.2 ml / €430.00
  OriGene Technologies GmbH

PTCH1 antibody
Detection of Patched in E13.5 Embryonic Mouse Spinal Cord. Tissue was stained using a rhodamine-conjugated anti-rat IgG secondary antibody (red) and the cell nuclei counterstained with DAPI (blue).  Rat IgG2a 413220 Purified Ms C, E, WB
0.1 mg / €340.00
  Neuromics Antibodies

PTCH1 (269-279) antibody
PTCH1 Rabbit Purified Chk, Hu, Ms, Rt WB
0.1 ml / €310.00
  Neuromics Antibodies

CRISPR

Catalog No. Species Donor Vector  

PTCH1 - human gene knockout kit via CRISPR
PTCH1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

PTCH1 - human gene knockout kit via CRISPR
PTCH1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

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29 products - FILTER YOUR RESULTS!

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Target
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  2. Mouse (Ms) (9)
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  • free call: 0800-227 47 46 (Germany only)
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