PTCH1 antibody
Principal name
PTCH1 antibody
Alternative names for PTCH1 antibody
PTCH, PTC1, PTC, Patched 1, Protein patched homolog 1
SwissProt ID
O42335 (Cynpy), Q09614 (Caeel), Q13635 (Human), Q61115 (Mouse), Q90693 (Chick), Q98864 (Danre)
Ncbi ID
NP_000255.2, 9606, 7955, AAR21240, NP_000255, NP_032983.1, NM_008957, NM_001083607, NM_000264, NM_001083603
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of PTCH1 antibody
PTCH (Patched protein homolog 1) is a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). PTCH associates with the smoothened protein (SMO) to transduce the hedgehog's proteins signal.PTCH has a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis. PTCH is expressed in the adult brain, lung, liver, heart, placenta, skeletal muscle, pancreas and kidney. It is also expressed in tumor cells but not in normal skin. During development PTCH is found in all major target tissues of sonic hedgehog, such as the ventral neural tube, somites, and tissues surrounding the zone of polarizing activity of the limb bud.
Defects in PTCH are probably the cause of basal cell nevus syndrome also known as Gorlin syndrome or Gorlin-Goltz syndrome.
General readings
1. Ju B, Spitsbergen J, Eden CJ, Taylor MR, Chen W.
Co-activation of hedgehog and AKT pathways promote tumorigenesis in zebrafish. Mol Cancer. 2009 Jun 25;8:40.
PMID: 19555497
Detection limit for recombinant GST tagged PTCH is approximately 0.03ng/ml as a capture antibody.
Detection limit for recombinant GST tagged PTCH is approximately 0.1ng/ml as a capture antibody.
Detection limit for recombinant GST tagged PTCH is approximately 1ng/ml as a capture antibody.
Detection limit for recombinant GST tagged PTCH is approximately 0.3ng/ml as a capture antibody.
Detection limit for recombinant GST tagged PTCH is approximately 1ng/ml as a capture antibody.
Detection limit for recombinant GST tagged PTCH is approximately 0.3ng/ml as a capture antibody.
Detection limit for recombinant GST tagged PTCH is approximately 1ng/ml as a capture antibody.
Immunohistochemistry (IHC) analysis of Patched antibody (Cat.-No.: AP06278PU-N) in paraffin-embedded human brain tissue.
Western blot (WB) analysis of Patched antibody (Cat.-No.: AP06278PU-N) at 1/500 dilution
Lane1:Hela whole cell lysate
Lane2:NIH-3T3 whole cell lysate
Lane3:PC12 whole cell lysate
Lane4:MCF-7 whole cell lysate
Lane1:Hela whole cell lysate
Lane2:NIH-3T3 whole cell lysate
Lane3:PC12 whole cell lysate
Lane4:MCF-7 whole cell lysate
PTCH1 polyclonal antibody ( Cat # PAB7063 ) ( 1 µg/ml ) staining of human brain lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
PTCH monoclonal antibody (M10), clone 3B3 Western Blot analysis of PTCH expression in Y-79 ( Cat # L042V1 ).
Human Kidney; Kidney; PTCH1 antibody - C-terminal region (ARP44249_P050) in Human Kidney cells using Immunohistochemistry
Human Jurkat; WB Suggested Anti-PTCH1 Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysate; PTCH1 antibody - C-terminal region (ARP44249_P050) in Human Jurkat cells using Western Blot
Western blot analysis of human PTCH1 in fetal brain lysate with PTCH1 polyclonal antibody ( Cat # PAB12543 ) at a 1 : 500 dilution.
AP22448PU-N PTCH1 antibody staining of Paraffin Embedded Human Colon at 3.8 µg/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry Image: Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunohistochemistry Image: Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)
Formalin-Fixed, Paraffin-Embedded Human Small Intestine stained with PTCH1 antibody Cat.-No AP31827PU-N
Western blot analysis of extracts from mouse muscle cells using PTCH1 antibody Cat.-No AP31827PU-N. The lane on the right is treated with the synthesized peptide.
Staining of Mouse fetal Kidney lysate using PTCH1 Antibody Cat.-No AP32805PU-N at 2 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot analysis of Patched expression in HeLa (A); Ramos (B); MCF7 (C); NIH3T3 (D); PC12 (E) whole cell lysates.
Anti-Patched1 mouse monoclonal antibody (TA500122) immunofluorescent staining of HeLa cells transiently transfected by pCMV6-ENTRY PTCH1(RC201597)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTCH1(RC216999) (RC216999, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTCH1.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PTCH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500123)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PTCH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500123)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PTCH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500123)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PTCH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500123)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PTCH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500123)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PTCH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500123)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PTCH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500123)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PTCH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500123)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PTCH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500123)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-PTCH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500123)
Anti-PTCH1 mouse monoclonal antibody (TA500123) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PTCH1(RC216999).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTCH1 (RC216999, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTCH1.
Immunohistochemistry: Patched 1 Antibody - With exception of malignant gliomas, malignant tissues such as in colo-rectal cancer showed in general a moderate to strong cytoplasmic and membranous positivity. Image was provided courtesy of the Swedish Human Proteome Resource (HPR) Center.
Immunohistochemistry: Patched 1 Antibody - Normal and malignant tissues showed in general a moderate to strong cytoplasmic and membranous positivity. Malignant gliomas were mostly weakly stained or negative. Stroma and endothelial lining were distinctly stained in normal and tumor tissues. Image and statement courtesy of the Human Protein Atlas (HPA).
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17; Lane 2: RT-4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: TonsilThis validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
ELISA: Patched 1 Antibody
Human recombinant protein fragment corresponding to amino acids 770-1027 of human PTCH1 (NP_000255) produced in E.coli.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTCH1 (RC216999, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTCH1.
Human recombinant protein fragment corresponding to amino acids 770-1027 of human PTCH1 (NP_000255) produced in E.coli.
Human recombinant protein fragment corresponding to amino acids 770-1027 of human PTCH1 (NP_000255) produced in E.coli.
TA303124 (1µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: Patched 1 Antibody -Analysis of Human PTCH1 in Fetal brain Lysate with anti huPTCH1 at a 1:500 dilution.
WB Suggested Anti-PTCH1 Antibody Titration: 0.2-1 ug/ml; Positive Control: Jurkat cell lysate
Kidney
Western blot analysis on mouse muscle cell lysate using Patched Antibody
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN211551LP | PTCH1 - human gene knockout kit via CRISPR |
||
 |
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
