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PTP4A2 antibody

Principal name

PTP4A2 antibody

Alternative names for PTP4A2 antibody

PRL2, PRL-2, PTPCAAX2, HU-PP-1, OV-1, Protein-tyrosine phosphatase 4a2, Protein tyrosine phosphatase type IVA 2, Protein-tyrosine phosphatase of regenerating liver 2

SwissProt ID

O70274 (Mouse), Q12974 (Human), Q6P9X4 (Rat)

Gene ID

8073 (PTP4A2), 19244 (Ptp4a2), 85237

Ncbi ID

NP_001182029.1, 9606, NP_536316, NM_080391, NP_001158217.1, NM_053475, NM_001195101

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of PTP4A2 antibody

The protein encoded by this gene belongs to a small class of the protein tyrosine phosphatase (PTP) family. PTPs are cell sigling molecules that play regulatory roles in a variety of cellular processes. PTPs in this class contain a protein tyrosine phosphatase catalytic domain and a characteristic C-termil prenylation motif. This PTP has been shown to primarily associate with plasmic and endosomal membrane through its C-termil prenylation. This PTP was found to interact with the beta-subunit of Rab geranylgeranyltransferase II (beta GGT II); and thus may function as a regulator of GGT II activity. Overexpression of this gene in mammalian cells conferred a transformed phenotype; which suggested its role in tumorigenesis. Altertively spliced transcript variants have been described. Related pseudogenes exist on chromosomes 11; 12 and 17.?

General readings

Si X., et al. (2001) J Biol chem. 246(35):32875-82.
Werner SR., et al. (2003) Cancer Lett. 202(2):201-11.

Recombinant human PTP4A2, 1- 167 aa, His-tagged

TP309983

Predicted band size: 19 kDa . Positive control: Jurkat cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 50 ug per lane Primary antibody: 1/300 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 seconds)

TA324078

Predicted band size: 19kDa. Positive control: Hela, Jurkat and SKOV3 cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lane1: Hela cell lysate Lane2: Jurkat cell lysate Lane3: SKOV3 cell lysate Lysates: 50 ug per lane Primary antibody: 1/300 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 30 seconds)

TA324079

Predicted cell location: Cytoplasm. Positive control: Human colon cancer tissue . Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using PTP4A2 antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification:x200)

TA324079

PTP4A2 Antibody (Center) (Cat. #TA324871) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PTP4A2 antibody detected the PTP4A2 protein (arrow).

TA324871

Formalin-fixed and paraffin-embedded human prostate carcinoma with PTP4A2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

TA324871

Immunofluorescence analysis of PTP4A2 Antibody (Center) with paraffin-embedded human prostate carcinoma tissue . 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.

TA324871

Flow cytometric analysis of widr cells using PTP4A2 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

TA324871

Lysates

Catalog No.
LY409184	PTP4A2 overexpression lysate
		0.1 mg / €295.00
		OriGene Technologies, Inc.

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