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RACGAP1 antibody

Principal name

RACGAP1 antibody

Alternative names for RACGAP1 antibody

MGCRACGAP, KIAA1478, Rac GTPase-activating protein 1, Male germ cell RacGap

SwissProt ID

Q9H0H5 (Human), Q9WVM1 (Mouse)

Gene ID

29127 (RACGAP1), 26934 (Racgap1)

Ncbi ID

9606, NP_037409, NP_001119575, NP_001119575.1, NP_001119576, NP_037409.2, NP_036155.1, BC010715, NM_012025, NM_013277, NM_001126103, NM_001126104

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rt (Rat), African clawed frog, Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Por (Porcine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Immunoprecipitation (IP)

Background of RACGAP1 antibody

Kit Component:: - KN314405G1, Racgap1 gRNA vector 1 in pCas-Guide vector
- KN314405G2, Racgap1 gRNA vector 2 in pCas-Guide vector
- KN314405D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:: CRISPR/CAS9 Genome Editing Manual

General readings

Niiya,F., et al., (2005) J. Biol. Chem. 280 (43), 36502-36509

Immunoperoxidase of monoclonal antibody to RACGAP1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 1ug/ml]

H00029127-M01

RACGAP1 monoclonal antibody (M01), clone 1G6. Western Blot analysis of RACGAP1 expression in Jurkat ( Cat # L017V1 ).

H00029127-M01

RACGAP1 monoclonal antibody (M01), clone 1G6. Western Blot analysis of RACGAP1 expression in 293 ( Cat # L026V1 ).

H00029127-M01

Detection limit for recombinant GST tagged RACGAP1 is approximately 0.3ng/ml as a capture antibody.

H00029127-M01

Human HepG2; WB Suggested Anti-RACGAP1 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:1562500. Positive Control: HepG2 cell lysate; RACGAP1 antibody - N-terminal region (ARP31905_T100) in Human HepG2 cells using Western Blot

ARP31905_T100

RACGAP1 polyclonal antibody ( Cat # PAB6112 ) staining ( 1 µg/ml ) of paraffin embedded human testis. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

PAB6112

RACGAP1 polyclonal antibody ( Cat # PAB6112 ) staining ( 0.2 µg/ml ) of human testis lysate ( RIPA buffer, 30 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

PAB6112

Human ACHN; WB Suggested Anti-RACGAP1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: ACHN cell lysate. RACGAP1 is supported by BioGPS gene expression data to be expressed in ACHN.; RACGAP1 antibody - N-terminal region (ARP32595_P050) in Human ACHN cells using Western Blot

ARP32595_P050

Immunohistochemistry analyzes of MgcRacGAP antibody (AP20256PU-N) in paraffin-embedded human brain tissue.

AP20256PU-N

Western blot analysis of MgcRacGAP antibody (AP20256PU-N) in extracts from COS7 cells.

AP20256PU-N

Immunohistochemistry analysis of paraffin-embedded human placenta, using GTPase Activating Protein (Phospho-Ser387) Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of HeLa cells, using GTPase Activating Protein (Phospho-Ser387) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from COS7 cells, using GTPase Activating Protein (Phospho-Ser387) Antibody. The lane on the right is treated with the synthesized peptide.

Immunofluorescence analysis of HeLa cells, using GTPase Activating Protein (Ab-387) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from COS7 cells, using GTPase Activating Protein (Ab-387) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using RGAP1 Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of COS7 cells, using RGAP1 Antibody. The picture on the right is treated with the synthesized peptide.

Formalin fixed, paraffin embedded human testis tissue stained with RACGAP1 Antibody (N-term) Cat.-No AP53556PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.

AP53556PU-N

Confocal immunofluorescent analysis of RACGAP1 Antibody (N-term) Cat.-No AP53556PU-N with MCF-7 cell followed by Alexa Fluor 488-conjugated Goat anti-Rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

AP53556PU-N

Western blot analysis of RACGAP1 Antibody (N-term) Cat.-No AP53556PU-N in MCF-7 cell line lysates (35ug/lane). This demonstrates the RACGAP1 antibody detected the RACGAP1 protein (arrow

AP53556PU-N

Flow Cytometric analysis of MCF-7 cells using RACGAP1 Antibody (N-term) Cat.-No AP53556PU-N (right histogram) compared to a Negative Control cell (left histogram). FITC-conjugated Goat-anti-Rabbit secondary antibodies were used for the analysis.

AP53556PU-N

Western blot analysis of extracts from COS7 tissue using GTPase Activating Protein antibody AP26452PU-N / AP26452PU-S. The lane on the right is treated with the antigen-specific peptide.

Immunohistochemical analysis of MGCRACGAP staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of MGCRACGAP staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot analysis of MGCRACGAP expression in Raji (A); K562 (B); A431 (C) whole cell lysates.

TP307542

TP326045

TP326046

TA302684 staining (0.2µg/ml) of Human Testis lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

TA302684

Sample (50 ug of whole cell lysate). A: mouse heart. 7.5% SDS PAGE. TA308788 diluted at 1:1000.

TA308788

Sample (30 ug of whole cell lysate). A: A431 . B: Raji. 7.5% SDS PAGE. TA308788 diluted at 1:1000.

TA308788

Immunohistochemical analysis of paraffin-embedded H1299 xenograft , using RACGAP1(TA308788) antibody at 1:500 dilution.

TA308788

Immunohistochemical analysis of paraffin-embedded Hepatoma, using RACGAP1(TA308788) antibody at 1:500 dilution.

TA308788

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using RACGAP1(TA308788) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with anti-alpha tubulin antibody (Red) at 1:2000.

TA308788

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using RGAP1 antibody.The picture on the right is treated with the synthesized peptide.

TA312359

Immunofluorescence analysis of COS-7 cells, using RGAP1 antibody.The picture on the right is treated with the synthesized peptide.

TA312359

Western blot analysis of GTPase Activating Protein expression in COS7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

TA325784

WB Suggested Anti-RACGAP1 Antibody Titration: 1.25ug/ml; ELISA Titer: 1:1562500; Positive Control: HepG2 cell lysate

TA329685

WB Suggested Anti-RACGAP1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: ACHN cell lysateRACGAP1 is supported by BioGPS gene expression data to be expressed in ACHN

TA330619

Western blot analysis of extracts of various cell lines, using RACGAP1 antibody.

TA332591

Immunofluorescence analysis of U20S cell using RACGAP1 antibody. Blue: DAPI for nuclear staining.

TA332591

Host: Rabbit; Target Name: Racgap1; Sample Tissue: Rat Stomach lysates; Antibody Dilution: 1.0 ug/ml

TA343711

Western blot analysis of GTPase Activating Protein phosphorylation expression in COS7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

TA325785

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
TA308788	RACGAP1 antibody
	Rabbit Polyclonal antibody to RACGAP1 (Rac GTPase activating protein 1)
	Rabbit	IgG	Aff - Purified	Hu, Ms	ICC/IF, P, WB	0.1 ml / €415.00
						OriGene Technologies, Inc.
+4 additional images	Highly validated antibody!
AP53556PU-N	RACGAP1 (N-term) antibody
	Rabbit	Ig	Aff - Purified	Hu	F, ICC/IF, P, WB	0.4 ml / €415.00
						OriGene Technologies GmbH
+3 additional images	Highly validated antibody!
TA302684	RACGAP1 (C-term) antibody
	Goat Polyclonal Antibody against RACGAP1 / MgcRacGAP
	Goat		Aff - Purified	Bov, Can, Hu	E, P, WB	0.1 mg / €360.00
						OriGene Technologies, Inc.
TA312359	RACGAP1 antibody
	Rabbit polyclonal GTPase Activating Protein antibody
	Rabbit	IgG	Purified	Hu, Ms	E, ICC/IF, P	0.1 ml / €335.00
						OriGene Technologies, Inc.
+1 additional image
TA325784	RACGAP1 antibody
	Rabbit Polyclonal GTPase Activating Protein Antibody
	Rabbit	IgG	Purified	Hu, Ms, Rt	WB	0.1 ml / €335.00
						OriGene Technologies, Inc.
TA325785	RACGAP1 antibody
	Rabbit Polyclonal GTPase Activating Protein (Ser387) Antibody (Phospho-specific)
	Rabbit	IgG	Purified	Hu, Ms, Rt	WB	0.1 ml / €335.00
						OriGene Technologies, Inc.
TA332591	RACGAP1 antibody
	Rabbit anti-RACGAP1 Polyclonal Antibody
	Rabbit	IgG	Purified	Hu, Ms, Rt		0.1 mg / €335.00
						OriGene Technologies, Inc.
+1 additional image
AP20256PU-N	RACGAP1 antibody
	Rabbit		Aff - Purified	Hu, Ms, Rt	ICC/IF, P, WB	0.1 mg / €370.00
						OriGene Technologies GmbH
+1 additional image
AP26452PU-N	RACGAP1 pSer387 antibody
	Rabbit		Aff - Purified	Hu, Ms, Rt	WB	0.1 mg / €300.00
						OriGene Technologies GmbH
AP26452PU-S	RACGAP1 pSer387 antibody
	Rabbit		Aff - Purified	Hu, Ms, Rt	WB	50 µg / €230.00
						OriGene Technologies GmbH

Proteins & Growth Factors

Catalog No.	Species	Purity	Source
TP307542	RACGAP1 (transcript variant 1)
	Recombinant protein of human Rac GTPase activating protein 1 (RACGAP1), transcript variant 1
	Human	> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
				OriGene Technologies, Inc.

TP326045	RACGAP1 (transcript variant 2)
	Purified recombinant protein of Homo sapiens Rac GTPase activating protein 1 (RACGAP1), transcript variant 2
	Human	> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
				OriGene Technologies, Inc.
TP326046	RACGAP1 (transcript variant 3)
	Recombinant protein of human Rac GTPase activating protein 1 (RACGAP1), transcript variant 3
	Human	> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
				OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.
MR209580L2V	Lenti ORF particles, Racgap1 (GFP-tagged) - Mouse Rac GTPase-activating protein 1 (cDNA clone MGC:11396 IMAGE:3602242), 200 uL, >10^7 TU/mL
		200 µl / €1,240.00
		OriGene Technologies, Inc.

MR216578L2V	Lenti ORF particles, Racgap1 (GFP-tagged) - Mouse Rac GTPase-activating protein 1 (Racgap1), 200 uL, >10^7 TU/mL
		200 µl / €1,360.00
		OriGene Technologies, Inc.

MR209580L1V	Lenti ORF particles, Racgap1 (Myc-DDK-tagged) - Mouse Rac GTPase-activating protein 1 (cDNA clone MGC:11396 IMAGE:3602242), 200 uL, >10^7 TU/mL
		200 µl / €1,110.00
		OriGene Technologies, Inc.

MR216578L1V	Lenti ORF particles, Racgap1 (Myc-DDK-tagged) - Mouse Rac GTPase-activating protein 1 (Racgap1), 200 uL, >10^7 TU/mL
		200 µl / €1,220.00
		OriGene Technologies, Inc.

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