RACGAP1 antibody
Principal name
RACGAP1 antibody
Alternative names for RACGAP1 antibody
MGCRACGAP, KIAA1478, Rac GTPase-activating protein 1, Male germ cell RacGap
Ncbi ID
9606, NP_037409, NP_001119575, NP_001119575.1, NP_001119576, NP_037409.2, NP_036155.1, BC010715, NM_012025, NM_013277, NM_001126103, NM_001126104
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Immunoprecipitation (IP)
Background of RACGAP1 antibody
- Kit Component:
- - KN314405G1, Racgap1 gRNA vector 1 in pCas-Guide vector
- KN314405G2, Racgap1 gRNA vector 2 in pCas-Guide vector
- KN314405D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Niiya,F., et al., (2005) J. Biol. Chem. 280 (43), 36502-36509
Immunoperoxidase of monoclonal antibody to RACGAP1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 1ug/ml]
RACGAP1 monoclonal antibody (M01), clone 1G6. Western Blot analysis of RACGAP1 expression in Jurkat ( Cat # L017V1 ).
RACGAP1 monoclonal antibody (M01), clone 1G6. Western Blot analysis of RACGAP1 expression in 293 ( Cat # L026V1 ).
Detection limit for recombinant GST tagged RACGAP1 is approximately 0.3ng/ml as a capture antibody.
Human HepG2; WB Suggested Anti-RACGAP1 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:1562500. Positive Control: HepG2 cell lysate; RACGAP1 antibody - N-terminal region (ARP31905_T100) in Human HepG2 cells using Western Blot
RACGAP1 polyclonal antibody ( Cat # PAB6112 ) staining ( 1 µg/ml ) of paraffin embedded human testis. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
RACGAP1 polyclonal antibody ( Cat # PAB6112 ) staining ( 0.2 µg/ml ) of human testis lysate ( RIPA buffer, 30 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Human ACHN; WB Suggested Anti-RACGAP1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: ACHN cell lysate. RACGAP1 is supported by BioGPS gene expression data to be expressed in ACHN.; RACGAP1 antibody - N-terminal region (ARP32595_P050) in Human ACHN cells using Western Blot
Immunohistochemistry analyzes of MgcRacGAP antibody (AP20256PU-N) in paraffin-embedded human brain tissue.
Western blot analysis of MgcRacGAP antibody (AP20256PU-N) in extracts from COS7 cells.
Formalin fixed, paraffin embedded human testis tissue stained with RACGAP1 Antibody (N-term) Cat.-No AP53556PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescent analysis of RACGAP1 Antibody (N-term) Cat.-No AP53556PU-N with MCF-7 cell followed by Alexa Fluor 488-conjugated Goat anti-Rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Western blot analysis of RACGAP1 Antibody (N-term) Cat.-No AP53556PU-N in MCF-7 cell line lysates (35ug/lane). This demonstrates the RACGAP1 antibody detected the RACGAP1 protein (arrow
Flow Cytometric analysis of MCF-7 cells using RACGAP1 Antibody (N-term) Cat.-No AP53556PU-N (right histogram) compared to a Negative Control cell (left histogram). FITC-conjugated Goat-anti-Rabbit secondary antibodies were used for the analysis.
Western blot analysis of extracts from COS7 tissue using GTPase Activating Protein antibody AP26452PU-N / AP26452PU-S. The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of MGCRACGAP staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of MGCRACGAP staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of MGCRACGAP expression in Raji (A); K562 (B); A431 (C) whole cell lysates.
TA302684 staining (0.2µg/ml) of Human Testis lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Sample (50 ug of whole cell lysate). A: mouse heart. 7.5% SDS PAGE. TA308788 diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: A431 . B: Raji. 7.5% SDS PAGE. TA308788 diluted at 1:1000.
Immunohistochemical analysis of paraffin-embedded H1299 xenograft , using RACGAP1(TA308788) antibody at 1:500 dilution.
Immunohistochemical analysis of paraffin-embedded Hepatoma, using RACGAP1(TA308788) antibody at 1:500 dilution.
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using RACGAP1(TA308788) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with anti-alpha tubulin antibody (Red) at 1:2000.
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using RGAP1 antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of COS-7 cells, using RGAP1 antibody.The picture on the right is treated with the synthesized peptide.
Western blot analysis of GTPase Activating Protein expression in COS7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
WB Suggested Anti-RACGAP1 Antibody Titration: 1.25ug/ml; ELISA Titer: 1:1562500; Positive Control: HepG2 cell lysate
WB Suggested Anti-RACGAP1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: ACHN cell lysateRACGAP1 is supported by BioGPS gene expression data to be expressed in ACHN
Western blot analysis of extracts of various cell lines, using RACGAP1 antibody.
Immunofluorescence analysis of U20S cell using RACGAP1 antibody. Blue: DAPI for nuclear staining.
Host: Rabbit; Target Name: Racgap1; Sample Tissue: Rat Stomach lysates; Antibody Dilution: 1.0 ug/ml
Western blot analysis of GTPase Activating Protein phosphorylation expression in COS7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP307542 | RACGAP1 (transcript variant 1) |
||||
| Recombinant protein of human Rac GTPase activating protein 1 (RACGAP1), transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP326045 | RACGAP1 (transcript variant 2) |
||||
| Purified recombinant protein of Homo sapiens Rac GTPase activating protein 1 (RACGAP1), transcript variant 2 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP326046 | RACGAP1 (transcript variant 3) |
||||
| Recombinant protein of human Rac GTPase activating protein 1 (RACGAP1), transcript variant 3 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
