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RAG2 antibody

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Principal name

RAG2 antibody

Alternative names for RAG2 antibody

RAG-2, V(D)J recombination-activating protein 2

SwissProt ID

O13034 (Danre), P21784 (Mouse), P25022 (Chick), P34089 (Rabit), P55895 (Human), Q91830 (Xenla)

Gene ID

5897 (RAG2), 19374 (Rag2)

Ncbi ID

9606, NP_000527.2, NM_000536, NM_009020, NP_033046.1

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse)

Available hosts

Mouse, Rabbit

Available applications

ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Enzyme Immunoassay (E), Paraffin Sections (P)

Background of RAG2 antibody

This gene encodes a protein that is involved in the initiation of V(D)J recombition during B and T cell development. This protein forms a complex with the product of the adjacent recombition activating gene 1, and this complex can form double-strand breaks by cleaving D at conserved recombition sigl sequences. The recombition activating gene 1 component is thought to contain most of the catalytic activity, while the N-termil of the recombition activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with D. A C-termil plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms. [provided by RefSeq]

H00005897-B01P - RAG2
Immunofluorescence of purified MaxPab antibody to RAG2 on HeLa cell. [antibody concentration 10 ug/ml]
H00005897-B01P - RAG2
Western Blot analysis of RAG2 expression in transfected 293T cell line (H00005897-T01) by RAG2 MaxPab polyclonal antibody.Lane 1: RAG2 transfected lysate(57.97 KDa).Lane 2: Non-transfected lysate.
H00005897-D01P - RAG2
Western Blot analysis of RAG2 expression in transfected 293T cell line (H00005897-T02) by RAG2 MaxPab polyclonal antibody.Lane 1: RAG2 transfected lysate(59.20 KDa).Lane 2: Non-transfected lysate.
H00005897-M06 - RAG2
Detection limit for recombinant GST tagged RAG2 is approximately 1ng/ml as a capture antibody.
AM06429SU-N - RAG2
Western blot analysis using RAG2 mouse mAb against RAG2(AA: 350-527)-hIgGFc transfected HEK293 (1)cell lysate.
TA307957 - RAG2
Sample (30 ug of whole cell lysate). A: Molt-4 . 7.5% SDS PAGE. TA307957 diluted at 1:1000.
TA307957 - RAG2
RAG2 antibody detects RAG2 protein by Western blot analysis. A. 50 ug Rat thymus lysate/extract. 7.5 % SDS-PAGE. RAG2 antibody (TA307957) dilution: 1:1000
TA307957 - RAG2
Immunohistochemical analysis of paraffin-embedded DLD1 Xenograft, using RAG2(TA307957) antibody at 1:100 dilution.
TA307957 - RAG2
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RAG2(TA307957) antibody at 1:200 dilution.
TA332798 - RAG2
Western blot analysis of extracts of various cell lines, using RAG2 antibody.
TA332798 - RAG2
Immunofluorescence analysis of U20S cell using RAG2 antibody. Blue: DAPI for nuclear staining.
TA352213 - RAG2
Western blot analysis of PBMC (lane 1), mouse thymus (lane 2) and Hela (lane 3) cells using anti-RAG2 antibody (3G4A26). GAPDH antibody (poly6314) was used as loading control.
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  2. Highly Validated (1)

4 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RAG2 antibody
  Rabbit polyclonal antibody to RAG2 (recombination activating gene 2)  
Sample (30 ug of whole cell lysate). A: Molt-4 . 7.5% SDS PAGE. TA307957 diluted at 1:1000. Rabbit IgG Aff - Purified Hu, Rt ICC/IF, P, WB
0.1 ml / €415.00
  OriGene Technologies, Inc.
+3 additional images

Highly validated antibody!

RAG2 antibody
  Rabbit anti-RAG2 Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using RAG2 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €335.00
  OriGene Technologies, Inc.
+1 additional image

RAG2 antibody
Western blot analysis using RAG2 mouse mAb against RAG2(AA: 350-527)-hIgGFc transfected HEK293 (1)cell lysate. Mouse IgG1 4D5 Ascites Hu E, WB
0.1 ml / €340.00
  OriGene Technologies GmbH

Lysates

Catalog No.    

RAG2 overexpression lysate
RAG2 overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.

4 Item(s)

per page

Available Filters

Product Type
  1. Lysate (1)
  2. Primary Antibody (3)
Clonality
  1. Polyclonal (2)
  2. Monoclonal (1)
Target
  1. RAG2 (3)
Reactivity
  1. Human (Hu) (3)
  2. Rat (Rt) (2)
  3. Mouse (Ms) (1)
Applications
  1. Western blot / Immunoblot (WB) (2)
  2. Enzyme Immunoassay (E) (1)
  3. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
  4. Paraffin Sections (P) (1)
Presentation
  1. Aff - Purified (1)
  2. Ascites (1)
  3. Purified (1)
Host
  1. Rabbit (2)
  2. Mouse (1)
Images
  1. 1 image (3)
  2. 3+ images (1)
Images: Applications
  1. Western blot / Immunoblot (WB) (3)
  2. Immunofluorescence (IF) (2)
  3. IHC (1)
Manufacturer
  1. OriGene Technologies (4)
Service
  • OriGene Technologies GmbH
  • Schillerstraße 5
  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
  • email: info-de@origene.com
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