SCAP antibody
Principal name
SCAP antibody
Alternative names for SCAP antibody
SREBP cleavage-activating protein, KIAA0199, Sterol regulatory element-binding protein cleavage-activating protein
SwissProt ID
A0JPH4 (Xenla), A2RRU4 (Rat), A6QM06 (Bovin), O43043 (Schpo), Q12770 (Human), Q5MNU5 (Pig), Q6GQT6 (Mouse)
Ncbi ID
NP_036367, NP_036367.2, NP_001001144.2, NM_001001144, NM_001103162, NM_012235
Available reactivities
Available hosts
Available applications
Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E)
Background of SCAP antibody
- Kit Component:
- - KN315339G1, Scap gRNA vector 1 in pCas-Guide vector
- KN315339G2, Scap gRNA vector 2 in pCas-Guide vector
- KN315339D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Immunohistochemistry analysis in Formalin Fixed, Paraffin Embedded Human stomach tissue stained with SCAP Antibody (N-term) Cat.-No AP53806PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of SCAP Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Western blot analysis of SCAP Antibody (N-term) Cat.-No AP53806PU-N in K562 cell line lysates (35ug/lane). This demonstrates the SCAP antibody detected the SCAP protein (arrow).
Western blot analysis of SCAP Antibody (Center) (Cat. #TA325047) in K562 cell line lysates (35ug/lane). SCAP (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human colon carcinoma with SCAP Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Fluorescent confocal image of HeLa cells stained with SCAP (Center) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with TA325047 SCAP (Center) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min).
Flow cytometric analysis of K562 cells using SCAP Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using (SCAP Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (SCAP Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200)
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC223734L2V | Lenti ORF particles, SCAP (mGFP-tagged) - Human SREBF chaperone (SCAP), 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€2,630.00
|
|
| OriGene Technologies, Inc. | ||
| RC223734L1V | Lenti ORF particles, SCAP (Myc-DDK tagged) - Human SREBF chaperone (SCAP) , 200 uL, >10^7 TU/mL |
|
|
200 µl /
€2,380.00
|
|
| OriGene Technologies, Inc. | ||