SDHB antibody
Principal name
SDHB antibody
Alternative names for SDHB antibody
SDH, SDH1, Iron-sulfur subunit of complex II, Succinate dehydrogenase [ubiquinone] iron-sulfur subunit mitochondrial
SwissProt ID
A5PL98 (Danre), P21911 (Schpo), P21912 (Human), P21913 (Rat), P21914 (Drome), Q007T0 (Pig), Q3T189 (Bovin), Q9CQA3 (Mouse), Q9YHT2 (Chick)
Ncbi ID
9606, NP_002991, NM_023374, NP_002991.2, NM_003000, NP_075863.2, NM_001100539
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)
Background of SDHB antibody
Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme. Sporadic and familial mutations in this gene result in paragangliomas and pheochromocytoma, and support a link between mitochondrial dysfunction and tumorigenesis.Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme. Sporadic and familial mutations in this gene result in paragangliomas and pheochromocytoma, and support a link between mitochondrial dysfunction and tumorigenesis. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
General readings
Selak MA, Armour SM, MacKenzie ED, Boulahbel H, Watson DG, Mansfield KD, Pan Y, Simon MC, Thompson CB, Gottlieb E. Succinate links TCA cycle dysfunction to oncogenesis by inhibiting HIF-alpha prolyl hydroxylase. Cancer Cell. 2005 Jan;7(1):77-85.
SDHB polyclonal antibody ( Cat # PAB6839 ) ( 0.03 µg/ml ) staining of human liver lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
SDHB MaxPab rabbit polyclonal antibody. Western Blot analysis of SDHB expression in human kidney.
Western Blot analysis of SDHB expression in transfected 293T cell line (H00006390-T04) by SDHB MaxPab polyclonal antibody.Lane 1: SDHB transfected lysate(31.60 KDa).Lane 2: Non-transfected lysate.
SDHB MaxPab rabbit polyclonal antibody. Western Blot analysis of SDHB expression in HeLa.
Hum. Fetal Liver; Host: Rabbit. Target Name: FAM46C . Sample Tissue: Human Fetal Liver . Antibody Dilution: 1.0ug/ml.; SDHB antibody - middle region (ARP47981_P050) in Hum. Fetal Liver cells using Western Blot
Hum. Fetal Heart; Host: Rabbit. Target Name: GNAS . Sample Tissue: Human Fetal Heart . Antibody Dilution: 1.0ug/ml.; SDHB antibody - middle region (ARP47981_P050) in Hum. Fetal Heart cells using Western Blot
Hum. Adult Placenta; Host: Rabbit. Target Name: SERPINA3 . Sample Tissue: Human Adult Placenta . Antibody Dilution: 1.0ug/ml.; SDHB antibody - middle region (ARP47981_P050) in Hum. Adult Placenta cells using Western Blot
Human Jurkat; WB Suggested Anti-SDHB Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Jurkat cell lysate. SDHB is supported by BioGPS gene expression data to be expressed in Jurkat.; SDHB antibody - middle region (ARP47981_P050) in Human Jurkat cells using Western Blot
Human Heart (formalin-fixed, paraffin-embedded) stained with SDHB antibody AP22481PU-N at 5 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Antibody (0.03 µg/ml) staining of Human Liver lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Mouse Heart; WB Suggested Anti-Sdhb Antibody. . Titration: 1.0 ug/ml. . Positive Control: Mouse Heart; Sdhb antibody - middle region (ARP47980_P050) in Mouse Heart cells using Western Blot
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SDHB mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808102)(1:150)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SDHB mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808102)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-SDHB mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808102)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-SDHB mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808102)(1:150)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SDHB mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808102)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SDHB (RC203182, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SDHB.(1:2000)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SDHB mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808109)(1:150)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SDHB mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808109)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-SDHB mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808109)(1:150)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SDHB mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808109)(1:150)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SDHB mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808109)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SDHB (RC203182, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SDHB.(1:2000)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SDHB (RC203182, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SDHB.(1:2000)
TA302600 (0.03µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
SDHB antibody [C2C3], C-term detects SDHB protein by Western blot analysis. A. 30 ug THP-1 whole cell lysate/extract. B. 30 ug NCI-H929 whole cell lysate/extract. 12 % SDS-PAGE. SDHB antibody [C2C3], C-term (TA308686) dilution: 1:1000
SDHB antibody [C2C3], C-term detects SDHB protein by Western blot analysis. A. 30 ug Neuro2A whole cell lysate/extract. 12 % SDS-PAGE. SDHB antibody [C2C3], C-term (TA308686) dilution: 1:1000
Immunofluorescence analysis of methanol-fixed HeLa, using SDHB(TA308686) antibody at 1:200 dilution.
Western Blot: SDHB Antibody [TA336808] - Detection of SDHB in mouse kidney lysate.
Immunohistochemistry: SDHB Antibody [TA336808] - IHC staining of SDHB in mouse bladder.
Immunocytochemistry/Immunofluorescence: SDHB Antibody [TA336808] - ICC staining of SDHB in HeLa cells with FITC (green). Nuclei were counterstained with Dapi (blue).
Host: Rabbit; Target Name: FAM46C; Sample Tissue: Human Fetal Liver; Antibody Dilution: 1.0 ug/ml
Host: Rabbit; Target Name: GNAS; Sample Tissue: Human Fetal Heart; Antibody Dilution: 1.0 ug/ml
Host: Rabbit; Target Name: SERPINA3; Sample Tissue: Human Adult Placenta; Antibody Dilution: 1.0 ug/ml
WB Suggested Anti-SDHB Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Jurkat cell lysate.SDHB is supported by BioGPS gene expression data to be expressed in Jurkat
- Quick-Filters:
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA808102 | SDHB antibody |
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| SDHB mouse monoclonal antibody, clone OTI3F5 (formerly 3F5) | |||||||
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Mouse | IgG1 | OTI3F5 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +5 additional images |
Highly validated antibody!
|
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| TA808109 | SDHB antibody |
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| SDHB mouse monoclonal antibody, clone OTI1H6 (formerly 1H6) | |||||||
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Mouse | IgG1 | OTI1H6 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +5 additional images |
Highly validated antibody!
|
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| TA302600 | SDHB (C-term) antibody |
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| Goat Polyclonal Antibody against SDHB | |||||||
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Goat | Aff - Purified | Hu | E, WB |
0.1 mg /
€360.00
|
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| OriGene Technologies, Inc. | |||||||
| TA308686 | SDHB antibody |
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| Rabbit Polyclonal antibody to SDHB (succinate dehydrogenase complex, subunit B, iron sulfur (Ip)) | |||||||
![SDHB antibody [C2C3], C-term detects SDHB protein by Western blot analysis. A. 30 ug THP-1 whole cell lysate/extract. B. 30 ug NCI-H929 whole cell lysate/extract. 12 % SDS-PAGE. SDHB antibody [C2C3], C-term (TA308686) dilution: 1:1000](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/GTX104628-1-W.jpg) |
Rabbit | IgG | Aff - Purified | Hu, Ms | ICC/IF, WB |
0.1 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA336808 | SDHB antibody |
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| Rabbit Polyclonal SDHB Antibody | |||||||
![Western Blot: SDHB Antibody [TA336808] - Detection of SDHB in mouse kidney lysate.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NBP1-54154-1-W.jpg) |
Rabbit | Purified | Hu, Ms | C, ICC/IF, P, WB |
0.1 ml /
€395.00
|
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| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA351639 | SDHB antibody |
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| Rabbit Polyclonal Anti-SDHB Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA808201 | SDHB antibody |
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| SDHB mouse monoclonal antibody, clone OTI2A3 (formerly 2A3) | |||||||
 |
Mouse | IgG2b | OTI2A3 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| AP22481PU-N | SDHB (271-280) antibody |
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 |
Goat | Aff - Purified | Hu | E, P, WB |
50 µg /
€425.00
|
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP760076 | SDHB (full length, N-term HIS tag) |
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| Recombinant protein of human succinate dehydrogenase complex, subunit B, iron sulfur (Ip) (SDHB), nuclear gene encoding mitochondrial protein, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug | |||||
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Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
€205.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY401052 | SDHB overexpression lysate |
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0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR203816L2V | Lenti ORF particles, Sdhb (GFP-tagged) - Mouse succinate dehydrogenase complex, subunit B, iron sulfur (Ip) (Sdhb), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
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200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR203816L1V | Lenti ORF particles, Sdhb (Myc-DDK-tagged) - Mouse succinate dehydrogenase complex, subunit B, iron sulfur (Ip) (Sdhb), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
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|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR210540L2V | Lenti ORF particles, Sdhb (GFP-tagged ORF) - Rat succinate dehydrogenase complex, subunit B, iron sulfur (Ip) (Sdhb), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR210540L1V | Lenti ORF particles, Sdhb (Myc-DDK-tagged ORF) - Rat succinate dehydrogenase complex, subunit B, iron sulfur (Ip) (Sdhb), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||