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SDHB antibody

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Principal name

SDHB antibody

Alternative names for SDHB antibody

SDH, SDH1, Iron-sulfur subunit of complex II, Succinate dehydrogenase [ubiquinone] iron-sulfur subunit mitochondrial

SwissProt ID

A5PL98 (Danre), P21911 (Schpo), P21912 (Human), P21913 (Rat), P21914 (Drome), Q007T0 (Pig), Q3T189 (Bovin), Q9CQA3 (Mouse), Q9YHT2 (Chick)

Gene ID

6390 (SDHB), 67680 (Sdhb), 298596

Ncbi ID

9606, NP_002991, NM_023374, NP_002991.2, NM_003000, NP_075863.2, NM_001100539

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Por (Porcine), Rb (Rabbit), Rt (Rat), Eq (Equine), Fi (Fish), Mky (Monkey), Prim (Primate), GP (Guinea Pig), Gt (Goat), Ye (Yeast), Ze (Zebrafish)

Available hosts

Goat, Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)

Background of SDHB antibody

Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme. Sporadic and familial mutations in this gene result in paragangliomas and pheochromocytoma, and support a link between mitochondrial dysfunction and tumorigenesis.Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme. Sporadic and familial mutations in this gene result in paragangliomas and pheochromocytoma, and support a link between mitochondrial dysfunction and tumorigenesis. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Selak MA, Armour SM, MacKenzie ED, Boulahbel H, Watson DG, Mansfield KD, Pan Y, Simon MC, Thompson CB, Gottlieb E. Succinate links TCA cycle dysfunction to oncogenesis by inhibiting HIF-alpha prolyl hydroxylase. Cancer Cell. 2005 Jan;7(1):77-85.

PAB6839 - SDHB
SDHB polyclonal antibody ( Cat # PAB6839 ) ( 0.03 µg/ml ) staining of human liver lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
H00006390-D01P - SDHB
SDHB MaxPab rabbit polyclonal antibody. Western Blot analysis of SDHB expression in human kidney.
H00006390-D01P - SDHB
Western Blot analysis of SDHB expression in transfected 293T cell line (H00006390-T04) by SDHB MaxPab polyclonal antibody.Lane 1: SDHB transfected lysate(31.60 KDa).Lane 2: Non-transfected lysate.
H00006390-D01P - SDHB
SDHB MaxPab rabbit polyclonal antibody. Western Blot analysis of SDHB expression in HeLa.
ARP47981_P050 - SDHB
Hum. Fetal Liver; Host: Rabbit. Target Name: FAM46C . Sample Tissue: Human Fetal Liver . Antibody Dilution: 1.0ug/ml.; SDHB antibody - middle region (ARP47981_P050) in Hum. Fetal Liver cells using Western Blot
ARP47981_P050 - SDHB
Hum. Fetal Heart; Host: Rabbit. Target Name: GNAS . Sample Tissue: Human Fetal Heart . Antibody Dilution: 1.0ug/ml.; SDHB antibody - middle region (ARP47981_P050) in Hum. Fetal Heart cells using Western Blot
ARP47981_P050 - SDHB
Hum. Adult Placenta; Host: Rabbit. Target Name: SERPINA3 . Sample Tissue: Human Adult Placenta . Antibody Dilution: 1.0ug/ml.; SDHB antibody - middle region (ARP47981_P050) in Hum. Adult Placenta cells using Western Blot
ARP47981_P050 - SDHB
Human Jurkat; WB Suggested Anti-SDHB Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Jurkat cell lysate. SDHB is supported by BioGPS gene expression data to be expressed in Jurkat.; SDHB antibody - middle region (ARP47981_P050) in Human Jurkat cells using Western Blot
AP22481PU-N - SDHB
Human Heart (formalin-fixed, paraffin-embedded) stained with SDHB antibody AP22481PU-N at 5 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
AP22481PU-N - SDHB
Antibody (0.03 µg/ml) staining of Human Liver lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
ARP47980_P050 - SDHB
Mouse Heart; WB Suggested Anti-Sdhb Antibody. . Titration: 1.0 ug/ml. . Positive Control: Mouse Heart; Sdhb antibody - middle region (ARP47980_P050) in Mouse Heart cells using Western Blot
TA808102 - SDHB
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SDHB mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808102)(1:150)
TA808102 - SDHB
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SDHB mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808102)(1:150)
TA808102 - SDHB
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-SDHB mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808102)(1:150)
TA808102 - SDHB
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-SDHB mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808102)(1:150)
TA808102 - SDHB
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SDHB mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808102)(1:150)
TA808102 - SDHB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SDHB (RC203182, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SDHB.(1:2000)
TA808109 - SDHB
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SDHB mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808109)(1:150)
TA808109 - SDHB
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SDHB mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808109)(1:150)
TA808109 - SDHB
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-SDHB mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808109)(1:150)
TA808109 - SDHB
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SDHB mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808109)(1:150)
TA808109 - SDHB
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SDHB mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808109)(1:150)
TA808109 - SDHB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SDHB (RC203182, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SDHB.(1:2000)
TA808201 - SDHB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SDHB (RC203182, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SDHB.(1:2000)
TP760076 - SDHB
TA302600 - SDHB
TA302600 (0.03µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA308686 - SDHB
SDHB antibody [C2C3], C-term detects SDHB protein by Western blot analysis. A. 30 ug THP-1 whole cell lysate/extract. B. 30 ug NCI-H929 whole cell lysate/extract. 12 % SDS-PAGE. SDHB antibody [C2C3], C-term (TA308686) dilution: 1:1000
TA308686 - SDHB
SDHB antibody [C2C3], C-term detects SDHB protein by Western blot analysis. A. 30 ug Neuro2A whole cell lysate/extract. 12 % SDS-PAGE. SDHB antibody [C2C3], C-term (TA308686) dilution: 1:1000
TA308686 - SDHB
Immunofluorescence analysis of methanol-fixed HeLa, using SDHB(TA308686) antibody at 1:200 dilution.
TA336808 - SDHB
Western Blot: SDHB Antibody [TA336808] - Detection of SDHB in mouse kidney lysate.
TA336808 - SDHB
Immunohistochemistry: SDHB Antibody [TA336808] - IHC staining of SDHB in mouse bladder.
TA336808 - SDHB
Immunocytochemistry/Immunofluorescence: SDHB Antibody [TA336808] - ICC staining of SDHB in HeLa cells with FITC (green). Nuclei were counterstained with Dapi (blue).
TA346485 - SDHB
Host: Rabbit; Target Name: FAM46C; Sample Tissue: Human Fetal Liver; Antibody Dilution: 1.0 ug/ml
TA346485 - SDHB
Host: Rabbit; Target Name: GNAS; Sample Tissue: Human Fetal Heart; Antibody Dilution: 1.0 ug/ml
TA346485 - SDHB
Host: Rabbit; Target Name: SERPINA3; Sample Tissue: Human Adult Placenta; Antibody Dilution: 1.0 ug/ml
TA346485 - SDHB
WB Suggested Anti-SDHB Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Jurkat cell lysate.SDHB is supported by BioGPS gene expression data to be expressed in Jurkat

5 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SDHB (C-term) antibody
  Goat Polyclonal Antibody against SDHB  
TA302600 (0.03µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €360.00
  OriGene Technologies, Inc.

SDHB antibody
  Rabbit Polyclonal antibody to SDHB (succinate dehydrogenase complex, subunit B, iron sulfur (Ip))  
SDHB antibody [C2C3], C-term detects SDHB protein by Western blot analysis. A. 30 ug THP-1 whole cell lysate/extract. B. 30 ug NCI-H929 whole cell lysate/extract. 12 % SDS-PAGE. SDHB antibody [C2C3], C-term (TA308686) dilution: 1:1000 Rabbit IgG Aff - Purified Hu, Ms ICC/IF, WB
0.1 ml / €415.00
  OriGene Technologies, Inc.
+2 additional images

SDHB antibody
  Rabbit Polyclonal SDHB Antibody  
Western Blot: SDHB Antibody [TA336808] - Detection of SDHB in mouse kidney lysate. Rabbit Purified Hu, Ms C, ICC/IF, P, WB
0.1 ml / €395.00
  OriGene Technologies, Inc.
+2 additional images

SDHB antibody
  Rabbit Polyclonal Anti-SDHB Antibody  
SDHB Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

SDHB (271-280) antibody
Human Heart (formalin-fixed, paraffin-embedded) stained with SDHB antibody AP22481PU-N at 5 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Goat Aff - Purified Hu E, P, WB
50 µg / €425.00
  OriGene Technologies GmbH
+1 additional image

5 Item(s)

per page

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Product Type
  1. Primary Antibody (5)
Target
  1. SDHB (5)
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  1. 271-280 (1)
  2. C-term (1)
Reactivity
  1. Human (Hu) (5)
  2. Mouse (Ms) (3)
  3. Rat (Rt) (1)
Applications
  1. Western blot / Immunoblot (WB) (5)
  2. Enzyme Immunoassay (E) (3)
  3. Paraffin Sections (P) (3)
  4. Immunocytochemistry/​Immunofluorescence (ICC/IF) (2)
  5. Frozen sections (C) (1)
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  2. Purified (2)
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  1. Rabbit (3)
  2. Goat (2)
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  1. 1 image (4)
  2. 3+ images (2)
Images: Applications
  1. Western blot / Immunoblot (WB) (4)
  2. Immunofluorescence (IF) (2)
  3. IHC (1)
  4. Paraffin Sections (P) (1)
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