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SEPHS2 antibody

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Principal name

SEPHS2 antibody

Alternative names for SEPHS2 antibody

Selenide water dikinase 2, Selenophosphate synthase 2, Selenium donor protein 2, SPS2

SwissProt ID

A1YIZ1 (Pig), P08459 (Yeast), P97364 (Mouse), Q99611 (Human), Q9VKY8 (Drome)

Gene ID

22928 (SEPHS2), 20768 (Sephs2)

Ncbi ID

9606, 10090, NP_036380, NP_036380.2, NP_033292.2, NM_009266, NM_012248

Available reactivities

Hu (Human), Ms (Mouse)

Available hosts

Rabbit, Mouse

Available applications

Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of SEPHS2 antibody

Kit Component:
- KN201830G1, SEPHS2 gRNA vector 1 in pCas-Guide vector
- KN201830G2, SEPHS2 gRNA vector 2 in pCas-Guide vector
- KN201830D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

AP13853PU-N - SEPHS2
Formalin-fixed and paraffin-embedded human cancer tissue (breast carcinoma) reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
AP13853PU-N - SEPHS2
Western blot analysis of anti-SEPSH2 Pab(AP13853PU-N) in HepG2 cell line lysate (35 µg/lane). SEPSH2(arrow) was detected using the purified Pab (1:50 dilution).
H00022928-M02 - SEPHS2
Immunoperoxidase of monoclonal antibody to SEPHS2 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml]
H00022928-M02 - SEPHS2
SEPHS2 monoclonal antibody (M02), clone 2G9 Western Blot analysis of SEPHS2 expression in K-562 ( Cat # L009V1 ).
H00022928-M02 - SEPHS2
Detection limit for recombinant GST tagged SEPHS2 is approximately 0.03ng/ml as a capture antibody.
PAB11323 - SEPHS2
Western blot using Sephs2 polyclonal antibody ( Cat # PAB11323 ) shows detection of Sephs2 in NIH/3T3 cells over-expressing this protein.No signal is seen in control lysates or in lysates from cells over-expressing the protein after pre-treatment with Sephs2 siRNA.Endogenous Sephs2 can be detected in mouse kidney, liver and testes tissue lysates.Partial cross-reactivity is seen against recombinant SPS1.The primary antibody was used at a 1 : 1000 dilution.Personal Communication, D. Hatfield, NCI, Bethesda, MD.
PAB2041 - SEPHS2
Formalin-fixed and paraffin-embedded human cancer tissue reacted with SEPHS2 polyclonal antibody ( Cat # PAB2041 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
PAB2041 - SEPHS2
Western blot analysis of SEPHS2 polyclonal antibody ( Cat # PAB2041 ) in HepG2 cell line lysate ( 35 µg/lane ) . SEPHS2 ( arrow ) was detected using the purified Polyclonal antibody ( 1 : 50 dilution ) .
TP301830 - SEPHS2
TA308431 - SEPHS2
Sample (30ug whole cell lysate). A:HeLa S3. 7.5% SDS PAGE. TA308431 diluted at 1:1000
TA308431 - SEPHS2
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SEPHS2(TA308431) antibody at 1:200 dilution.
TA319460 - SEPHS2
WB using anti-SPS2 antibody shows detection of SPS2 in NIH3T3 cells over-expressing this protein. No signal is seen in control lysates or in lysates from cells over-expressing the protein after pre-treatment with SPS2 siRNA. Endogenous SPS2 can be detected in mouse kidney, liver and testes tissue lysates. Partial cross-reactivity is seen against recombinant SPS1. The primary antibody was used at a 1:1000 dilution. Personal Communication, D. Hatfield, NCI, Bethesda, MD.

6 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SEPHS2 antibody
  Rabbit polyclonal antibody to SEPHS2 (selenophosphate synthetase 2)  
Sample (30ug whole cell lysate). A:HeLa S3. 7.5% SDS PAGE. TA308431 diluted at 1:1000 Rabbit IgG Aff - Purified Hu ICC/IF, WB
0.1 ml / €415.00
  OriGene Technologies, Inc.
+1 additional image

SEPHS2 antibody
  Rabbit polyclonal Selenophosphate Synthetase 2 antibody  
WB using anti-SPS2 antibody shows detection of SPS2 in NIH3T3 cells over-expressing this protein. No signal is seen in control lysates or in lysates from cells over-expressing the protein after pre-treatment with SPS2 siRNA. Endogenous SPS2 can be detected in mouse kidney, liver and testes tissue lysates. Partial cross-reactivity is seen against recombinant SPS1. The primary antibody was used at a 1:1000 dilution. Personal Communication, D. Hatfield, NCI, Bethesda, MD. Rabbit IgG Purified Hu, Ms E, WB
0.1 mg / €515.00
  OriGene Technologies, Inc.

SEPHS2 (Center) antibody
Formalin-fixed and paraffin-embedded human cancer tissue (breast carcinoma) reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+1 additional image

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SEPHS2
  Purified recombinant protein of Homo sapiens selenophosphate synthetase 2 (SEPHS2)  
SEPHS2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.
 

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Txnrd1 (GFP-tagged) - Mouse thioredoxin reductase 1 (Txnrd1), transcript variant 4, (Note: selenocysteine protein, Internal stop codon present. see reference data summary below), 200 uL, >10^7 TU/mL
Lenti ORF particles, Txnrd1 (GFP-tagged) - Mouse thioredoxin reductase 1 (Txnrd1), transcript variant 4, (Note: selenocysteine protein, Internal stop codon present. see reference data summary below), 200 uL, >10^7 TU/mL  
200 µl / €1,110.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Txnrd1 (Myc-DDK-tagged) - Mouse thioredoxin reductase 1 (Txnrd1), transcript variant 4, (Note: selenocysteine protein, Internal stop codon present. see reference data summary below), 200 uL, >10^7 TU/mL
Lenti ORF particles, Txnrd1 (Myc-DDK-tagged) - Mouse thioredoxin reductase 1 (Txnrd1), transcript variant 4, (Note: selenocysteine protein, Internal stop codon present. see reference data summary below), 200 uL, >10^7 TU/mL  
200 µl / €1,000.00
  OriGene Technologies, Inc.
 

6 Item(s)

per page

Available Filters

Product Type
  1. Mouse Lenti ORF Particle (2)
  2. Primary Antibody (3)
  3. Protein | Growth Factor (1)
Clonality
  1. Polyclonal (3)
Target
  1. SEPHS2 (4)
Property
  1. Center (1)
Reactivity
  1. Human (Hu) (3)
  2. Mouse (Ms) (1)
Applications
  1. Western blot / Immunoblot (WB) (3)
  2. Enzyme Immunoassay (E) (1)
  3. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
  4. Paraffin Sections (P) (1)
Presentation
  1. Purified (2)
  2. Aff - Purified (1)
Host
  1. Rabbit (3)
Images
  1. 1 image (4)
Images: Applications
  1. Western blot / Immunoblot (WB) (3)
  2. Immunofluorescence (IF) (1)
  3. Paraffin Sections (P) (1)
Species (Protein)
  1. Human (1)
Source
  1. HEK293 cells (1)
Manufacturer
  1. OriGene Technologies (6)
Service
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