SEPHS2 antibody
Principal name
SEPHS2 antibody
Alternative names for SEPHS2 antibody
Selenide water dikinase 2, Selenophosphate synthase 2, Selenium donor protein 2, SPS2
Ncbi ID
9606, 10090, NP_036380, NP_036380.2, NP_033292.2, NM_009266, NM_012248
Available reactivities
Available hosts
Available applications
Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of SEPHS2 antibody
- Kit Component:
- - KN201830G1, SEPHS2 gRNA vector 1 in pCas-Guide vector
- KN201830G2, SEPHS2 gRNA vector 2 in pCas-Guide vector
- KN201830D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Formalin-fixed and paraffin-embedded human cancer tissue (breast carcinoma) reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of anti-SEPSH2 Pab(AP13853PU-N) in HepG2 cell line lysate (35 µg/lane). SEPSH2(arrow) was detected using the purified Pab (1:50 dilution).
Immunoperoxidase of monoclonal antibody to SEPHS2 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml]
SEPHS2 monoclonal antibody (M02), clone 2G9 Western Blot analysis of SEPHS2 expression in K-562 ( Cat # L009V1 ).
Detection limit for recombinant GST tagged SEPHS2 is approximately 0.03ng/ml as a capture antibody.
Western blot using Sephs2 polyclonal antibody ( Cat # PAB11323 ) shows detection of Sephs2 in NIH/3T3 cells over-expressing this protein.No signal is seen in control lysates or in lysates from cells over-expressing the protein after pre-treatment with Sephs2 siRNA.Endogenous Sephs2 can be detected in mouse kidney, liver and testes tissue lysates.Partial cross-reactivity is seen against recombinant SPS1.The primary antibody was used at a 1 : 1000 dilution.Personal Communication, D. Hatfield, NCI, Bethesda, MD.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with SEPHS2 polyclonal antibody ( Cat # PAB2041 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
Western blot analysis of SEPHS2 polyclonal antibody ( Cat # PAB2041 ) in HepG2 cell line lysate ( 35 µg/lane ) . SEPHS2 ( arrow ) was detected using the purified Polyclonal antibody ( 1 : 50 dilution ) .
Sample (30ug whole cell lysate). A:HeLa S3. 7.5% SDS PAGE. TA308431 diluted at 1:1000
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SEPHS2(TA308431) antibody at 1:200 dilution.
WB using anti-SPS2 antibody shows detection of SPS2 in NIH3T3 cells over-expressing this protein. No signal is seen in control lysates or in lysates from cells over-expressing the protein after pre-treatment with SPS2 siRNA. Endogenous SPS2 can be detected in mouse kidney, liver and testes tissue lysates. Partial cross-reactivity is seen against recombinant SPS1. The primary antibody was used at a 1:1000 dilution. Personal Communication, D. Hatfield, NCI, Bethesda, MD.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA308431 | SEPHS2 antibody |
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| Rabbit polyclonal antibody to SEPHS2 (selenophosphate synthetase 2) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu | ICC/IF, WB |
0.1 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| TA319460 | SEPHS2 antibody |
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| Rabbit polyclonal Selenophosphate Synthetase 2 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, WB |
0.1 mg /
€515.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP13853PU-N | SEPHS2 (Center) antibody |
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 |
Rabbit | Ig | Purified | Hu | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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