Sialidase-3 antibody
Principal name
Sialidase-3 antibody
Alternative names for Sialidase-3 antibody
NEU3, Membrane sialidase, Ganglioside sialidase, N-acetyl-alpha-neuraminidase 3
SwissProt ID
Q9UQ49 (Human)
Gene ID
10825 (NEU3)
Ncbi ID
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunoprecipitation (IP)
Background of Sialidase-3 antibody
- Kit Component:
- - KN216537G1, NEU3 gRNA vector 1 in pCas-Guide vector
- KN216537G2, NEU3 gRNA vector 2 in pCas-Guide vector
- KN216537D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Western blot analysis of NEU3 expression in Caco-2 cells (1) and NEU3 transfected DLD-1 cells (2) using AM26497AF-N.
Western blot analysis of NEU3 expression in mock transfected COS-1 cells (1) and NEU3 transfected COS-1 cells (2) using AM26497AF-N.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEU3 (RC216537, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEU3.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NEU3 polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
ELISA: NEU3 Antibody
Host: Rabbit; Target Name: NEU3; Sample Tissue: THP-1 Whole Cell lysates; Antibody Dilution: 1.0 ug/ml
WB analysis using Anti-SH3BP2 pS427 antibody to detect endogenous protein present in unstimulated human whole cell lysates). The band as indicated by the arrowheads is evident in both M059 cells (panel A) and PC-3 cells (panel B). Comparison to a molecular weight marker indicates a band of ~60 kDa corresponding to human SH3BP2 protein. The blot was incubated with a 1:500 dilution of the antibody at RTfollowed by detection using standard techniques.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA319445 | Sialidase-3 antibody |
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| Rabbit polyclonal SH3BP2 phospho S427 antibody (Phospho-specific) | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, IP, WB |
0.1 mg /
€515.00
|
|
| OriGene Technologies, Inc. | |||||||
| AM26497AF-N | Sialidase-3 antibody |
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 |
Mouse | IgG2a | 11B | Azide Free | Hu | WB |
0.1 mg /
€400.00
|
| OriGene Technologies GmbH | |||||||
 +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP316537 | Sialidase-3 |
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| Recombinant protein of human sialidase 3 (membrane sialidase) (NEU3) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
