SLC25A31 antibody
Principal name
SLC25A31 antibody
Alternative names for SLC25A31 antibody
AAC4, ANT4, ANT 4, ANT-4, SFEC, Solute carrier family 25 member 31, ADP/ATP translocase 4, Adenine nucleotide translocator 4, ADP ATP carrier protein 4, Sperm flagellar energy carrier protein
Ncbi ID
9606, NP_112581, NP_112581.1, NM_178386, NM_031291, NP_848473.2
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P)
Background of SLC25A31 antibody
- Kit Component:
- - KN315978G1, Slc25a31 gRNA vector 1 in pCas-Guide vector
- KN315978G2, Slc25a31 gRNA vector 2 in pCas-Guide vector
- KN315978D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Kim,Y.H., (2007) Dev. Biol. 302 (2), 463-476
Human Brain; WB Suggested Anti-SLC25A31 Antibody Titration: 0.2-1 ug/ml. Positive Control: Human brain; SLC25A31 antibody - N-terminal region (ARP53805_P050) in Human Brain cells using Western Blot
Western Blot analysis of SLC25A31 expression in transfected 293T cell line (H00083447-T02) by SLC25A31 MaxPab polyclonal antibody.Lane 1: SLC25A31 transfected lysate(35.00 KDa).Lane 2: Non-transfected lysate.
Mouse Heart; WB Suggested Anti-Slc25a31 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Mouse Heart; Slc25a31 antibody - middle region (ARP53806_P050) in Mouse Heart cells using Western Blot
Immunofluorescent analysis of ANT4 staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of ANT4 expression in K562 (A); U251MG (B) whole cell lysates.
Western blot analysis of extracts from COLO cells, HUVEC cells and HeLa cells, using SLC25A31 antibody.The lane on the right is treated with the synthesized peptide.
SLC25A31 Antibody (Center) (Cat. #TA324461) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the SLC25A31 antibody detected the SLC25A31 protein (arrow).
SLC25A31 Antibody (Center) (Cat. #TA324461)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SLC25A31 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Confocal immunofluorescent analysis of SLC25A31 Antibody (Center)(Cat#TA324461) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
WB Suggested Anti-Slc25a31 Antibody; Titration: 1.0 ug/ml; Positive Control: Mouse Heart
WB Suggested Anti-SLC25A31 Antibody Titration: 0.2-1 ug/ml; Positive Control: Human brain
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA315869 | SLC25A31 antibody |
||||||
| Rabbit polyclonal anti-SLC25A31 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA324461 | SLC25A31 antibody |
||||||
| Rabbit polyclonal SLC25A31 Antibody (Center) | |||||||
 |
Rabbit | IgG | Purified | Hu, Mky | ICC/IF, P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
|||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP760296 | SLC25A31 (full length, N-term HIS tag) |
||||
| Recombinant protein of human solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 31 (SLC25A31), nuclear gene encoding mitochondrial protein, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug | |||||
 |
Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
€205.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY410565 | SLC25A31 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |