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SLC25A31 antibody

Principal name

SLC25A31 antibody

Alternative names for SLC25A31 antibody

AAC4, ANT4, ANT 4, ANT-4, SFEC, Solute carrier family 25 member 31, ADP/ATP translocase 4, Adenine nucleotide translocator 4, ADP ATP carrier protein 4, Sperm flagellar energy carrier protein

SwissProt ID

Q2YDD9 (Bovin), Q3V132 (Mouse), Q9H0C2 (Human)

Gene ID

83447 (SLC25A31), 73333 (Slc25a31)

Ncbi ID

9606, NP_112581, NP_112581.1, NM_178386, NM_031291, NP_848473.2

Available reactivities

Bov (Bovine), Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey), Eq (Equine), Por (Porcine), Rb (Rabbit), GP (Guinea Pig)

Available hosts

Rabbit

Available applications

Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P)

Background of SLC25A31 antibody

Kit Component:: - KN315978G1, Slc25a31 gRNA vector 1 in pCas-Guide vector
- KN315978G2, Slc25a31 gRNA vector 2 in pCas-Guide vector
- KN315978D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:: CRISPR/CAS9 Genome Editing Manual

General readings

Kim,Y.H., (2007) Dev. Biol. 302 (2), 463-476

Human Brain; WB Suggested Anti-SLC25A31 Antibody Titration: 0.2-1 ug/ml. Positive Control: Human brain; SLC25A31 antibody - N-terminal region (ARP53805_P050) in Human Brain cells using Western Blot

ARP53805_P050

Western Blot analysis of SLC25A31 expression in transfected 293T cell line (H00083447-T02) by SLC25A31 MaxPab polyclonal antibody.Lane 1: SLC25A31 transfected lysate(35.00 KDa).Lane 2: Non-transfected lysate.

H00083447-D01P

Western blot analysis of extracts from HeLa/HuvEc/COLO cells, using SLC25A31 Antibody. The lane on the right is treated with the synthesized peptide.

Mouse Heart; WB Suggested Anti-Slc25a31 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Mouse Heart; Slc25a31 antibody - middle region (ARP53806_P050) in Mouse Heart cells using Western Blot

ARP53806_P050

Immunofluorescent analysis of ANT4 staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot analysis of ANT4 expression in K562 (A); U251MG (B) whole cell lysates.

TP760296

Western blot analysis of extracts from COLO cells, HUVEC cells and HeLa cells, using SLC25A31 antibody.The lane on the right is treated with the synthesized peptide.

TA315869

SLC25A31 Antibody (Center) (Cat. #TA324461) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the SLC25A31 antibody detected the SLC25A31 protein (arrow).

TA324461

SLC25A31 Antibody (Center) (Cat. #TA324461)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SLC25A31 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

TA324461

Confocal immunofluorescent analysis of SLC25A31 Antibody (Center)(Cat#TA324461) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).