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SPTLC1 antibody

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Principal name

SPTLC1 antibody

Alternative names for SPTLC1 antibody

Serine palmitoyltransferase 1, SPT 1, SPT1, LCB 1, LCB1, Serine-palmitoyl-CoA transferase 1, Long chain base biosynthesis protein 1

SwissProt ID

O15269 (Human), O35704 (Mouse), Q3MHG1 (Bovin)

Gene ID

10558 (SPTLC1)

Ncbi ID

9606, NP_006406, NP_006406.1, NM_178324, NM_006415

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Chk (Chicken), Rt (Rat), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Ye (Yeast), Por (Porcine)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Frozen Sections (C), Enzyme Immunoassay (E), Paraffin Sections (P)

Background of SPTLC1 antibody

Serine palmitoyltransferase, which consists of two different subunits, is the key enzyme in sphingolipid biosynthesis. It converts L-serine and palmitoyl-CoA to 3-oxosphinganine with pyridoxal 5\'-phosphate as a cofactor. SPTLC1 is the long chain base subunit 1 of serine palmitoyltransferase. Mutations in SPTLC1 gene were identified in patients with hereditary sensory neuropathy type 1. Serine palmitoyltransferase, which consists of two different subunits, is the key enzyme in sphingolipid biosynthesis. It converts L-serine and palmitoyl-CoA to 3-oxosphinganine with pyridoxal 5\'-phosphate as a cofactor. The product of this gene is the long chain base subunit 1 of serine palmitoyltransferase. Mutations in this gene were identified in patients with hereditary sensory neuropathy type 1. Alternatively spliced variants encoding different isoforms have been identified.

General readings

1. Dawkins, J. L., Hulme, D. J., Brahmbhatt, S. B., Auer-Grumbach, M., Nicholson, G. A.Mutations in
SPTLC1, encoding serine palmitoyltransferase, long chain base subunit-1, cause hereditary
sensory neuropathy type I.Nature Genet. 27: 309-312, 2001.
2. McCampbell, A., Truong, D., Broom, D. C., Allchorne, A., Gable, K., Cutler, R. G., Mattson, M. P.,
Woolf, C. J., Frosch, M. P., Harmon, J. M., Dunn, T. M., Brown, R. H., Jr.Mutant SPTLC1 dominantly
inhibits serine palmitoyltransferase activity in vivo and confers an age-dependent neuropathy.Hum.
Molec. Genet. 14: 3507-3521, 2005.
3. Verhoeven, K., Coen, K., De Vriendt, E., Jacobs, A., Van Gerwen, V., Smouts, I., Pou-Serradell, A.,
Martin, J.-J., Timmerman, V., De Jonghe, P.SPTLC1 mutation in twin sisters with hereditary sensory
neuropathy type I.Neurology 62: 1001-1002, 2004.

AP05280PU-N - SPTLC1
Immunohistochemical staining of normal Human lung tissue with Serine Palmitoyltransferase 1 antibody (Cat.-No AP05280PU-N) at 15 μg/ml.
AP05280PU-N - SPTLC1
Western blot on human kidney lysate (10 µg/lane) using Serine Palmitoyltransferase 1 antibody (Cat.-No AP05280PU-N) at 1 µg/ml. Blot was developed using anti rabbit HRP at 1:75k and Supersignal West Femto. Exposure was 2 minutes.
PAB2501 - SPTLC1
The SPTLC1 polyclonal antibody ( Cat # PAB2501 ) is used in Western blot to detect SPTLC1 in 293 cell lysate.
PAB4378 - SPTLC1
The SPTLC1 polyclonal antibody ( Cat # PAB4378 ) is used in Western blot to detect SPTLC1 in mouse liver tissue lysate .
PAB4378 - SPTLC1
The SPTLC1 polyclonal antibody ( Cat # PAB4378 ) is used in Western blot to detect SPTLC1 in HeLa cell lysate .
ARP46721_P050 - SPTLC1
Human kidney; SPTLC1 antibody - middle region (ARP46721_P050) in Human kidney cells using Immunohistochemistry
ARP46721_P050 - SPTLC1
Human HepG2; WB Suggested Anti-SPTLC1 Antibody Titration: 0.5ug/ml. Positive Control: HepG2 cell lysate; SPTLC1 antibody - middle region (ARP46721_P050) in Human HepG2 cells using Western Blot
AP12258PU-N - SPTLC1
Western blot analysis of anti-SPTLC1 Antibody (N-term)(Cat.#AP12258PU-N) in 293 cell line lysates (35ug/lane). SPTLC1 (arrow) was detected using the purified Pab.
AP12259PU-N - SPTLC1
Western blot analysis of anti-SPTLC1 Antibody (C-term)(Cat.#AP12259PU-N) in 293 cell line lysates (35ug/lane). SPTLC1 (arrow) was detected using the purified Pab.
ARP46722_P050 - SPTLC1
Human Brain; WB Suggested Anti-SPTLC1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human brain; SPTLC1 antibody - middle region (ARP46722_P050) in Human Brain cells using Western Blot
TP322985 - SPTLC1
TA319826 - SPTLC1
Western blot analysis of SPT1 in human lung tissue lysate with SPT1 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.
TA319826 - SPTLC1
Immunohistochemistry of SPT1 in human lung tissue with SPT1 antibody at 5 ug/mL.
TA339044 - SPTLC1
WB Suggested Anti-SPTLC1 Antibody Titration: 0.5 ug/ml; Positive Control: HepG2 cell lysate
TA339044 - SPTLC1
Human kidney
TA339045 - SPTLC1
WB Suggested Anti-SPTLC1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Human brain

7 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SPTLC1 (C-term) antibody
  Rabbit Polyclonal SPT1 Antibody  
Western blot analysis of SPT1 in human lung tissue lysate with SPT1 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide. Rabbit Aff - Purified Hu E, WB
0.1 mg / €415.00
  OriGene Technologies, Inc.
+1 additional image

SPTLC1 antibody
SPTLC1 Rabbit IgG Purified Hu WB
0.1 mg / €400.00
  OriGene Technologies GmbH

SPTLC1 antibody
Immunohistochemical staining of normal Human lung tissue with Serine Palmitoyltransferase 1 antibody (Cat.-No AP05280PU-N) at 15 μg/ml. Rabbit IgG Aff - Purified Hu C, WB
0.1 mg / €400.00
  OriGene Technologies GmbH
+1 additional image

SPTLC1 (N-term) antibody
Western blot analysis of anti-SPTLC1 Antibody (N-term)(Cat.#AP12258PU-N) in 293 cell line lysates (35ug/lane). SPTLC1 (arrow) was detected using the purified Pab. Rabbit Ig Purified Hu, Ms WB
0.4 ml / €415.00
  OriGene Technologies GmbH

SPTLC1 (C-term) antibody
Western blot analysis of anti-SPTLC1 Antibody (C-term)(Cat.#AP12259PU-N) in 293 cell line lysates (35ug/lane). SPTLC1 (arrow) was detected using the purified Pab. Rabbit Ig Purified Hu WB
0.4 ml / €415.00
  OriGene Technologies GmbH

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SPTLC1 (transcript variant 1)
  Recombinant protein of human serine palmitoyltransferase, long chain base subunit 1 (SPTLC1), transcript variant 1  
SPTLC1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.
 

Lysates

Catalog No.    

SPTLC1 overexpression lysate
SPTLC1 overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.

7 Item(s)

per page

Available Filters

Product Type
  1. Lysate (1)
  2. Primary Antibody (5)
  3. Protein | Growth Factor (1)
Clonality
  1. Polyclonal (5)
Target
  1. SPTLC1 (6)
Property
  1. C-term (2)
  2. N-term (1)
Reactivity
  1. Human (Hu) (5)
  2. Mouse (Ms) (1)
Applications
  1. Western blot / Immunoblot (WB) (5)
  2. Frozen sections (C) (1)
  3. Enzyme Immunoassay (E) (1)
Presentation
  1. Purified (3)
  2. Aff - Purified (2)
Host
  1. Rabbit (5)
Images
  1. 1 image (5)
Images: Applications
  1. Western blot / Immunoblot (WB) (4)
  2. Frozen sections (C) (1)
  3. IHC (1)
Species (Protein)
  1. Human (1)
Source
  1. HEK293 cells (1)
Manufacturer
  1. OriGene Technologies (7)
Service
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