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TIE1 antibody

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Principal name

TIE1 antibody

Alternative names for TIE1 antibody

TIE, Tie-1, Tyrosine-protein kinase receptor Tie-1

SwissProt ID

P35590 (Human), Q06805 (Bovin), Q06806 (Mouse)

Gene ID

7075 (TIE1)

Ncbi ID

NP_005415.1, 9606, NP_005415, 10090, NM_005424

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Rt (Rat)

Available hosts

Goat, Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Immunoprecipitation (IP)

Background of TIE1 antibody

TIE1/TIE (tyrosine kinase with Ig and EGF homology domains 1) and TIE2/Tek define a new class of the receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin like domains flanking three epidermal growth factor (EFG) like domains followed by three fibronectin type III like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. Human TIE1 cDNA encodes a 1138 amino acid residue precursor protein with a putative signal peptide, an extracellular domain, and a cytoplasmic domain. Human TIE1/Fc, a disulfide linked homodimeric protein, has a calculated molecular mass of approximately 107 kDa. Due to glycosylation, the protein migrates to approximately 160 kDa in SDS PAGE under reducing conditions. TIE1 and TIE2, expressed primarily on endothelial and hematopoietic progenitor cells, play important roles in angiogenesis, vasculogenesis, and hematopoiesis. In developing vascular endothelial cells, TIE1 and TIE2 are specifically expressed. Two ligands that bind TIE have been identified, angiopoietin 1 and angiopoietin 2. Based on gene targeting studies, the in vivo functions of TIE1 are related to endothelial cell differentiation. The receptor tyrosine kinase TIE also plays a role in the survival and integrity of the endothelium.

DM3517B - TIE1
FACS analysis with primary human dermal microvascular endothelial cells (HDMVEC). Upper panel: no primary antibody; Middle panel: solely conjugated secondary antibody; Lower panel: Biotin-conjugated anti-Human TIE-1.
H00007075-M01 - TIE1
Detection limit for recombinant GST tagged TIE1 is approximately 1ng/ml as a capture antibody.
H00007075-M03 - TIE1
Detection limit for recombinant GST tagged TIE1 is approximately 1ng/ml as a capture antibody.
PAB3397 - TIE1
Formalin-fixed and paraffin-embedded human brain tissue reacted with TIE1 polyclonal antibody ( Cat # PAB3397 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
PAB3397 - TIE1
Western blot analysis of TIE1 polyclonal antibody ( Cat # PAB3397 ) in mouse brain lysate. TIE ( arrow ) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
AP14431PU-N - TIE1
Formalin-fixed and paraffin-embedded human brain tissue reacted with TIE antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
AP14431PU-N - TIE1
Western blot analysis of anti-TIE Antibody (N-term) (Cat#AP14431PU-N) in mouse bladder tissue lysates (35 µg/lane). TIE (arrow) was detected using the purified Pab.
AP15820PU-S - TIE1
Human tonsil stained with Anti-Tie-1
PAB11825 - TIE1
Immunohistochemical staining of TIE1 on formalin fixed, paraffin embedded human tonsil with TIE1 polyclonal antibody.
AP31041PU-N - TIE1
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
AP31726PU-N - TIE1
Western analysis of recombinant Human and Mouse sTIE-1 and sTIE-2 using a Polyclonal antibody directed against Mouse recombinant sTIE-1. There is a strong cross reactivity with Human sTIE-1 but only a weak one for Human and Mouse sTIE-2 visible.
AM31937PU-N - TIE1
Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
AR31151PU-N - TIE1
SDS-PAGE analysis of recombinant mouse soluble TIE-1/Fc produced in CHO cells. Sample was loaded in 10% SDS-polyacrylamide gel under reducing condition and stained with Coomassie blue.
CPA2164-100ul - TIE1
Western blot analysis of TIE1 expression in A549 (A); ECV304 (B); NIH3T3 (C) whole cell lysates.
TA590602 - TIE1
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TIE1 (RC207771, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TIE1.
TA590602 - TIE1
ELISA: TIE1 Antibody
TP307771 - TIE1
TP700156 - TIE1
TP720418 - TIE1
TA314624 - TIE1
Western blot analysis of extracts from HepG2 cells, K562 cells and Jurkat cells, using TIE1 antibody.The lane on the right is treated with the synthesized peptide.

18 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TIE1 antibody
  Rabbit polyclonal anti-TIE1 antibody  
Western blot analysis of extracts from HepG2 cells, K562 cells and Jurkat cells, using TIE1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

TIE1 antibody
  Rabbit Polyclonal TIE1 Antibody  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TIE1 (RC207771, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TIE1. Rabbit IgG Purified Hu E, WB
0.1 mg / €409.00
  OriGene Technologies, Inc.
+1 additional image

TIE1 antibody
Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 1G10 Purified Hu E, P, WB
50 µg / €475.00
  OriGene Technologies GmbH

TIE1 (N-term) antibody
Formalin-fixed and paraffin-embedded human brain tissue reacted with TIE antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+1 additional image

TIE1 (C-term) antibody
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Purified Hu P
50 µl / €425.00
  OriGene Technologies GmbH

TIE1 antibody
Western analysis of recombinant Human and Mouse sTIE-1 and sTIE-2 using a Polyclonal antibody directed against Mouse recombinant sTIE-1. There is a strong cross reactivity with Human sTIE-1 but only a weak one for Human and Mouse sTIE-2 visible. Rabbit IgG Purified Hu, Ms WB
0.2 mg / €370.00
  OriGene Technologies GmbH

TIE1 antibody
Western analysis of recombinant Human and Mouse sTIE-1 and sTIE-2 using a Polyclonal antibody directed against Mouse recombinant sTIE-1. There is a strong cross reactivity with Human sTIE-1 but only a weak one for Human and Mouse sTIE-2 visible. Rabbit IgG Purified Hu, Ms WB
0.1 mg / €270.00
  OriGene Technologies GmbH

TIE1 antibody
FACS analysis with primary human dermal microvascular endothelial cells (HDMVEC). Upper panel: no primary antibody; Middle panel: solely conjugated secondary antibody; Lower panel: Biotin-conjugated anti-Human TIE-1. Mouse IgG1 6F12 Biotin Hu E, F, WB
50 µg / €330.00
  OriGene Technologies GmbH

TIE1 antibody
TIE1 Mouse IgG1 6F12 Purified Hu E, F, WB
0.1 mg / €330.00
  OriGene Technologies GmbH

TIE1 (Extracell. Dom.) antibody
TIE1 Mouse IgG1 8C9 Purified Hu E, F, WB
0.1 mg / €330.00
  OriGene Technologies GmbH

TIE1 (Extracell. Dom.) antibody
Tie-1 staining of Paraffin-embedded tissue sections of human placenta were stained with anti-goat HRP-DAB (brown) and Haematoxylin (blue) counterstain. Goat Aff - Purified Hu E, P, WB
0.1 mg / €450.00
  Neuromics Antibodies

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

TIE1
  Recombinant protein of human tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (TIE1)  
TIE1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €788.00
  OriGene Technologies, Inc.

TIE1 (C-term DDK/His tag)
  Purified Recombinant protein of human tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (TIE1), with C-terminal DDK/His tag, expressed in human cells, 20 µg  
TIE1 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

TIE1
  Recombinant protein of human tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (TIE1)  
TIE1 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
10 µg / €299.00
  OriGene Technologies, Inc.

TIE1 (C-term, His-tag)
TIE1 Mouse Purified > 90 % Insect cells
50 µg / €350.00
  OriGene Technologies GmbH

TIE1 (Fc Chimera)
SDS-PAGE analysis of recombinant mouse soluble TIE-1/Fc produced in CHO cells. Sample was loaded in 10% SDS-polyacrylamide gel under reducing condition and stained with Coomassie blue. Mouse Purified > 90 % by SDS-PAGE and visualised by silver stain CHO cells
0.1 mg / €350.00
  OriGene Technologies GmbH

TIE1 (Fc Chimera)
TIE1 Human Purified > 90 % by SDS-PAGE and visualised by silver stain. Insect cells
0.1 mg / €350.00
  OriGene Technologies GmbH

Lysates

Catalog No.    

TIE1 overexpression lysate
TIE1 overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.

18 Item(s)

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18 products - FILTER YOUR RESULTS!

Available Filters

Product Type
  1. Lysate (1)
  2. Primary Antibody (11)
  3. Protein | Growth Factor (6)
Clonality
  1. Polyclonal (7)
  2. Monoclonal (4)
Target
  1. TIE1 (17)
Property
  1. C-term (1)
  2. Extracell. Dom. (2)
  3. N-term (1)
Reactivity
  1. Human (Hu) (11)
  2. Mouse (Ms) (4)
Applications
  1. Western blot / Immunoblot (WB) (10)
  2. Enzyme Immunoassay (E) (7)
  3. Paraffin Sections (P) (4)
  4. Flow cytometry (F) (3)
Presentation
  1. Purified (12)
  2. Aff - Purified (1)
  3. Biotin (1)
Host
  1. Rabbit (6)
  2. Mouse (4)
  3. Goat (1)
Images
  1. 1 image (13)
Images: Applications
  1. Western blot / Immunoblot (WB) (5)
  2. Paraffin Sections (P) (3)
  3. Enzyme Immunoassay (E) (1)
  4. Flow cytometry (F) (1)
Species (Protein)
  1. Human (4)
  2. Mouse (2)
Source
  1. HEK293 cells (3)
  2. Insect cells (2)
  3. CHO cells (1)
Manufacturer
  1. OriGene Technologies (17)
  2. Neuromics Antibodies (1)
Service
  • OriGene Technologies GmbH
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  • D-32052 Herford
  • Germany
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  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
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