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TNFAIP3 antibody

Principal name

TNFAIP3 antibody

Alternative names for TNFAIP3 antibody

OTUD7C, Zinc finger protein A20, Tumor necrosis factor alpha-induced protein 3, OTU domain-containing protein 7C, Putative DNA-binding protein A20

SwissProt ID

P21580 (Human), Q60769 (Mouse)

Gene ID

7128 (TNFAIP3)

Ncbi ID

9606, NP_006281.1, NP_006281, NM_006290

Available reactivities

Hu (Human), Ms (Mouse), Bt (Bat), Bov (Bovine), Hst (Hamster), Mky (Monkey), Por (Porcine), Prim (Primate), Rb (Rabbit), Rt (Rat), Can (Canine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Flow Cytometry (F), Immunoprecipitation (IP)

Background of TNFAIP3 antibody

A20, also referred to as TNF-a-induced protein 3 (TNFAIP3), is cytokine-inducible protein that functions to inhibit apoptosis and activate NF-?B. It was first identified as a TNF-a inducible primary response gene in human umbilical vein endothelial cells, and encodes a 790-amino acid protein containing seven Cys2/Cys2-zinc finger motifs. Constitutive expression of A20 is observed in lymphoid tissues, but it is transiently expressed in a variety of cell types in response to inflammatory sigls such as TNF-a, IL-1, phorbol esters, and LPS . Expression of A20 can confer resistance to apoptosis and NF-?B activation triggered by these sigls, probably through interference with TRAF (TNF receptor associated factor) family members , and interaction with the NF-?B inhibiting protein ABIN. Studies also show that A20 contains site-specific ubiquitin modifying activity that can contribute to its biological functions. The amino-terminus of A20 contains de-ubiquititing (DUB) activity for Lys63 branches, such as those found in TRAF6 and RIP, while the carboxyl-terminus contains ubiquitin ligase (E3) activity for Lys48 branches of the same substrates and leads to their degradation .

Detection of TNFAIP3 by Western blot in HT1080 cells, wild type. Or transfected with TNFAIP3 were activated with 0.1 µg/mL of rhs TNF-a for 4 hours. TNFAIP3 monoclonal antibody, clone 8E8.38 ( Cat # MAB3716 ) at 1 : 1,000 dilution.

MAB3716

Immunofluorescence analysis of HepG2 cells, using TNAP3 Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from RAW264.7 cells, using TNAP3 Antibody. The lane on the right is treated with the synthesized peptide.

Small intestine, Human: Formalin-Fixed, Paraffin-Embedded (FFPE)

AP22993PU-N

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)

AP23477PU-N

Western blot analysis of TNFAIP3 in SK-N-SH cell lysate with TNFAIP3 antibody at 1 µg/ml.

AP23477PU-N

Immunohistochemical analysis of A20 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of A20 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot analysis of A20 expression in HeLa (A); Daudi (B); RAW264.7 (C) whole cell lysates.

TP321337

Western blot analysis of TNFAIP3 in SK-N-SH cell lysate with TNFAIP3 antibody at 1 ug/ml.

TA306764

Immunohistochemistry of TNFAIP3 in human lung tissue with TNFAIP3 antibody at 5 ug/ml.

TA306764

Immunofluorescence of TNFAIP3 in Human Lung cells with TNFAIP3 antibody at 20 ug/mL.

TA306764

Western blot analysis of TNFAIP3 in rat lung tissue lysate with TNFAIP3 antibody at (A) 1 and (B) 2 ug/ml.

TA306770

Immunohistochemistry of TNFAIP3 in human lung tissue with TNFAIP3 antibody at 5 ug/ml.

TA306770

Immunofluorescence of TNFAIP3 in Human Lung cells with TNFAIP3 antibody at 20 ug/mL.

TA306770

Western blot analysis of extracts from RAW264.7 cells, using TNAP3 antibody.The lane on the right is treated with the synthesized peptide.

TA312809

Immunofluorescence analysis of HepG2 cells, using TNAP3 antibody.The picture on the right is treated with the synthesized peptide.

TA312809

Western blot analysis of extracts of various mouse tissues, using TNFAIP3 antibody

TA327286

Immunohistochemistry of paraffin-embedded human stomach cancer using TNFAIP3 antibody at dilution of 1:200 (400x lens).

TA327286

Immunofluorescence analysis of U2OS cell using TNFAIP3 antibody. Blue: DAPI for nuclear staining.

TA327286

Western Blot: TNFAIP3 Antibody (59A426) [TA336834] - analysis of A20 using mAb 59A426. A20 migrated as a stack of bands and LPS treatment (200 ng, ) induced a ~58 kDa putative A20 cleavage band* not seen in the untreated control © THP1 cells. A20

TA336834

Western Blot: TNFAIP3 Antibody (59A426) [TA336834] - Human Jurkat lysate probed with A20 antibody at 4 ug/ml.

TA336834

Immunohistochemistry-Paraffin: TNFAIP3 Antibody (59A426) [TA336834] - Formalin-fixed, paraffin-embedded human placenta probed with A20 antibody at 5 ug/ml, cytoplasmic staining of decidual cells is observed. Human tissue TMA was used for this test.Stainin

TA336834

Flow Cytometry: TNFAIP3 Antibody (59A426) [TA336834] - Intracellular flow cytometric analysis of A20 in ThP-1 cells using A20 antibody (red) and isotype control (green) at 0.5 ug/ml

TA336834

Host: Rabbit; Target Name: TNFAIP3; Sample Tissue: ACHN whole cell lysates; Antibody Dilution: 1.0 ug/ml

TA343148

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
TA306764	TNFAIP3 (C-term) antibody
	Rabbit Polyclonal TNFAIP3 Antibody
	Rabbit	IgG		Aff - Purified	Hu, Ms, Rt	E, P, WB	0.1 mg / €415.00
							OriGene Technologies, Inc.
+2 additional images
TA306770	TNFAIP3 (Center) antibody
	Rabbit Polyclonal TNFAIP3 Antibody
	Rabbit	IgG		Aff - Purified	Hu, Ms, Rt	E, P, WB	0.1 mg / €415.00
							OriGene Technologies, Inc.
+2 additional images
TA312809	TNFAIP3 antibody
	Rabbit polyclonal anti-TNAP3 antibody
	Rabbit	IgG		Purified	Hu, Ms	E, ICC/IF, WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
+1 additional image
TA327286	TNFAIP3 antibody
	TNFAIP3 Rabbit Polyclonal Antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt		0.1 mg / €335.00
							OriGene Technologies, Inc.
+2 additional images
TA336834	TNFAIP3 antibody
	Mouse Monoclonal A20/TNFAIP3 Antibody (59A426)
	Mouse	IgG1	59A426	Purified	Hu, Ms, Rt	F, ICC/IF, IP, P, WB	0.1 mg / €465.00
							OriGene Technologies, Inc.
+3 additional images
AP22993PU-N	TNFAIP3 antibody
	Goat			Aff - Purified	Hu, Mky, Rb	P	50 µg / €425.00
							OriGene Technologies GmbH
AP23477PU-N	TNFAIP3 (C-term) antibody
	Rabbit				Hu, Ms, Rt	E, P, WB	50 µg / €425.00
							OriGene Technologies GmbH
+1 additional image

CRISPR

Catalog No.	Species	Donor Vector
KN221337	TNFAIP3 - human gene knockout kit via CRISPR
	Human	GFP-Puro	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN221337BN	TNFAIP3 - human gene knockout kit via CRISPR
	Human	mBFP-Neo	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN221337LP	TNFAIP3 - human gene knockout kit via CRISPR
	Human	Luciferase-Puro	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN221337RB	TNFAIP3 - human gene knockout kit via CRISPR
	Human	RFP-BSD	1 kit / €1,290.00
			OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No.	Species	Purity	Source
TP321337	TNFAIP3
	Recombinant protein of human tumor necrosis factor, alpha-induced protein 3 (TNFAIP3)
	Human	> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
				OriGene Technologies, Inc.

Lysates

Catalog No.
LY416749	TNFAIP3 overexpression lysate
		0.1 mg / €495.00
		OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.
RC221337L2V	Lenti ORF particles, TNFAIP3 (mGFP-tagged) - Human tumor necrosis factor, alpha-induced protein 3 (TNFAIP3), 200 uL, >10^7 TU/mL
		200 µl / €1,110.00
		OriGene Technologies, Inc.

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