TNFAIP3 antibody

Background of TNFAIP3 antibody

A20, also referred to as TNF-a-induced protein 3 (TNFAIP3), is cytokine-inducible protein that functions to inhibit apoptosis and activate NF-?B. It was first identified as a TNF-a inducible primary response gene in human umbilical vein endothelial cells, and encodes a 790-amino acid protein containing seven Cys2/Cys2-zinc finger motifs. Constitutive expression of A20 is observed in lymphoid tissues, but it is transiently expressed in a variety of cell types in response to inflammatory sigls such as TNF-a, IL-1, phorbol esters, and LPS . Expression of A20 can confer resistance to apoptosis and NF-?B activation triggered by these sigls, probably through interference with TRAF (TNF receptor associated factor) family members , and interaction with the NF-?B inhibiting protein ABIN. Studies also show that A20 contains site-specific ubiquitin modifying activity that can contribute to its biological functions. The amino-terminus of A20 contains de-ubiquititing (DUB) activity for Lys63 branches, such as those found in TRAF6 and RIP, while the carboxyl-terminus contains ubiquitin ligase (E3) activity for Lys48 branches of the same substrates and leads to their degradation .