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TSLP antibody

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Principal name

TSLP antibody

Alternative names for TSLP antibody

Thymic stromal lymphopoietin, Thymic stroma-derived lymphopoietin

SwissProt ID

Q969D9 (Human), Q9JIE6 (Mouse)

Gene ID

85480 (TSLP), 53603 (Tslp)

Ncbi ID

9606, NP_067342.1, NP_149024.1, NP_149024, NP_067342, NP_612561.1, NM_138551, NM_033035, NM_021367

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Rat, Mouse

Available applications

Paraffin Sections (P), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Frozen Sections (C), ELISA (capture) (E(capture)), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of TSLP antibody

The eBio15B11.3 monoclol antibody reacts with human thymic stromal lymphopoietin (TSLP). TSLP is an IL-7-like cytokine derived from epithelial cells, which strongly activates dendritic cells (DC). TSLP can replace the activity of IL-7 in supporting the development of B cells in vitro; it promotes the proliferation and differentiation of committed B220(+) B cell progenitors. TSLP prevents cell death by apoptosis and stimulates growth of the human acute myeloid leukemia cell line MUTZ-3. This effect is neutralized by antibodies directed against IL-7. The TSLP receptor has been shown to be a member of the hematopoietin receptor superfamily. High affinity binding sites require the presence of the alpha chain of the IL-7 receptor (CD127). The receptor complex does not involve the common gamma chain (CD132), a sigl-transducing component of various cytokine receptors, including receptors for IL-4, IL-7, IL-9, IL-13, and IL-15. Recently TSLP has been found expressed by keratinocytes of atopic dermatitis; TSLP expression is also associated with Langerhans cell migration and activation in situ. TSLP expressed within thymus and peripheral lymphoid and non-lymphoid tissues regulates DC-mediated central tolerance, peripheral T cell homeostasis, and inflammatory Th2 responses. DCs activated by TSLP can prime ?ve CD4 T cells to differentiate into T helper type 2 cells that produce high levels of TNF-α, but no IL-10. This priming for Th2 differentiation is dependent upon TSLP-mediated upregulation of OX40L by DCs.

General readings

Sims, J.E. et al. (2000) J. Exp. Med. 192:671.

Park, L.S. et al. (2000) J. Exp. Med. 192:659.

Pandey, A. et al. (2000) Nature Immunol. 1:59.

Reche, P.A. et al. (2001) J. Immunol. 167:336.

AP01155PU-N - TSLP
Formalin-Fixed, Paraffin-Embedded Human Sections prostate stained with TSLP Antibody Cat.-No AP01155PU at 0.125 µg/ml with an overnight incubation at 4°C.  An HRP-labeled polymer detection system was used with a DAB chromogen.  Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.
AP01155PU-N - TSLP
Western Blot (Reduced) using TSLP Antibody Cat.-No AP01155PU
AP01155PU-N - TSLP
Western Blot (Unreduced) using TSLP Antibody Cat.-No AP01155PU
AP01155PU-N - TSLP
Sandwich ELISA using TSLP Antibody Cat.-No AP01155PU
AP07802PU-N - TSLP
Figure 1. Staining TSLP in Thymus by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue.
PAB13434 - TSLP
Immunohistochemistry of TSLP in human brain tissue with TSLP polyclonal antibody ( Cat # PAB13434 ) at 2.5 µg/ml.
PAB13434 - TSLP
Western blot analysis of TSLP in Jurkat cell lysate with TSLP polyclonal antibody ( Cat # PAB13434 ) at ( A ) 1 and ( B ) 2 µg/ml.
PAB13435 - TSLP
Immunohistochemistry of TSLP in human brain tissue with TSLP polyclonal antibody ( Cat # PAB13435 ) at 2.5 µg/ml.
PAB13435 - TSLP
Western blot analysis of TSLP in A-20 cell lysate with TSLP polyclonal antibody ( Cat # PAB13435 ) at ( A ) 0.5, ( B ) 1 and ( C ) 2 µg/ml.
AP22662PU-N - TSLP
Human Prostate (formalin-fixed, paraffin-embedded) stained with TSLP antibody AP22662PU-N at 2.5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
AP22662PU-N - TSLP
Human Liver (formalin-fixed, paraffin-embedded) stained with TSLP antibody AP22662PU-N at 2.5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
AP22662PU-N - TSLP
Human Brain, Cortex (formalin-fixed, paraffin-embedded) stained with TSLP antibody AP22662PU-N at 2.5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
AP22662PU-N - TSLP
Western blot analysis of TSLP in A-20 cell lysate with TSLP antibody (AP22662PU-N) at (A) 0.5, (B) 1 and (C) 2 µg/ml.
AP22809PU-N - TSLP
Mouse Liver (formalin-fixed, paraffin-embedded) stained with TSLP antibody AP22809PU-N followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
AP22809PU-N - TSLP
Mouse Spleen (formalin-fixed, paraffin-embedded) stained with TSLP antibody AP22809PU-N followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
AP22809PU-N - TSLP
Western blot analysis of TSLP in A-20 cell lysate with TSLP antibody (AP22809PU-N) at (A) 0.5, (B) 1 and (C) 2 µg/ml.
DDX0800P-50 - TSLP
IHC staining of human tiisue frozen section with clone 214E4 (DX0480)
DDX0801P-50 - TSLP
IHC staining of human tonsil frozen section with clone209A2 (DX0481)
DDX0801P-50 - TSLP
Capture Elisa: 209A2.03 (3μg/ml) and 210G1-biot (4μg/ml).
DDX0804P-50 - TSLP
DDX0804P-50 - TSLP
AP54374PU-N - TSLP
TSLP Antibody (C-term) (AP54374PU-N) western blot analysis in Jurkat cell line lysates (35 µg/lane). This demonstrates the TSLP antibody detected the TSLP protein (arrow).
AR51770PU-N - TSLP
CEK1354 - Human TSLP ELISA Kit
Representative Standard Curve
CEK1568 - Mouse TSLP ELISA Kit
Representative Standard Curve
TP723462 - TSLP
TA306384 - TSLP
Western blot analysis of TSLP in Jurkat cell lysate with TSLP antibody at (A) 1 and (B) 2 ug/ml.
TA306384 - TSLP
Immunohistochemistry of TSLP in human brain tissue with TSLP antibody at 2.5 ug/ml.
TA306385 - TSLP
Western blot analysis of TSLP in A-20 cell lysate with TSLP antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.
TA306385 - TSLP
Immunohistochemistry of TSLP in human brain tissue with TSLP antibody at 2.5 ug/ml.
TA306385 - TSLP
Immunofluorescence of TSLP in Human Brain tissues with TSLP antibody at 20 ug/mL.
TA306386 - TSLP
Western blot analysis of TSLP in A-20 cell lysate with TSLP antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.
TA306386 - TSLP
Immunohistochemistry of TSLP in mouse brain tissue with TSLP antibody at 2.5 ug/ml.
TA306386 - TSLP
Immunofluorescence of TSLP in Mouse Brain tissue with TSLP antibody at 20 ug/mL.
TA320508 - TSLP
Standard curve of Human TSLP ELISA.
TA322122 - TSLP
Predicted band size: 18 kDa . Positive control: Jurkat cell lysate. Recommended dilution: 1/500-2000. (Gel: 16+12+10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/850 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes)
TA328465 - TSLP
To detect hTSLP by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human TSLP as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTSLP.
TA328465 - TSLP
To detect hTSLP by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human TSLP as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTSLP.
TA328465 - TSLP
To detect hTSLP by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human TSLP as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTSLP.
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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TSLP antibody
Formalin-Fixed, Paraffin-Embedded Human Sections prostate stained with TSLP Antibody Cat.-No AP01155PU at 0.125 µg/ml with an overnight incubation at 4°C.  An HRP-labeled polymer detection system was used with a DAB chromogen.  Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. Rabbit Aff - Purified Hu E, P, WB
0.1 mg / €340.00
  OriGene Technologies GmbH
+3 additional images

Highly validated antibody!

TSLP antibody
Formalin-Fixed, Paraffin-Embedded Human Sections prostate stained with TSLP Antibody Cat.-No AP01155PU at 0.125 µg/ml with an overnight incubation at 4°C.  An HRP-labeled polymer detection system was used with a DAB chromogen.  Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. Rabbit Aff - Purified Hu E, P, WB
50 µg / €250.00
  OriGene Technologies GmbH
+3 additional images

Highly validated antibody!

TSLP antibody
Human Prostate (formalin-fixed, paraffin-embedded) stained with TSLP antibody AP22662PU-N at 2.5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Purified Hu, Ms E, P, WB
50 µg / €425.00
  OriGene Technologies GmbH
+3 additional images

Highly validated antibody!

TSLP (C-term) antibody
  Rabbit Polyclonal TSLP Antibody  
Western blot analysis of TSLP in Jurkat cell lysate with TSLP antibody at (A) 1 and (B) 2 ug/ml. Rabbit IgG Aff - Purified Hu E, P, WB
0.1 mg / €415.00
  OriGene Technologies, Inc.
+1 additional image

TSLP (Center) antibody
  Rabbit Polyclonal TSLP Antibody  
Western blot analysis of TSLP in A-20 cell lysate with TSLP antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml. Rabbit IgG Aff - Purified Hu, Ms E, P, WB
0.1 mg / €415.00
  OriGene Technologies, Inc.
+2 additional images

TSLP antibody
  Rabbit Polyclonal TSLP Antibody  
Western blot analysis of TSLP in A-20 cell lysate with TSLP antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml. Rabbit IgG Aff - Purified Ms, Rt E, ICC/IF, P, WB
0.1 mg / €415.00
  OriGene Technologies, Inc.
+2 additional images

TSLP antibody
  Anti-TSLP Rabbit Polyclonal Antibody  
Predicted band size: 18 kDa . Positive control: Jurkat cell lysate. Recommended dilution: 1/500-2000. (Gel: 16+12+10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/850 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

TSLP antibody
  Anti-Human TSLP Rabbit Polyclonal Antibody  
To detect hTSLP by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human TSLP as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTSLP. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €275.00
  OriGene Technologies, Inc.
+2 additional images

TSLP antibody
TSLP Rabbit Biotin Hu E, WB
50 µg / €340.00
  OriGene Technologies GmbH

TSLP antibody
TSLP Rabbit Biotin Hu E, WB
25 µg / €250.00
  OriGene Technologies GmbH

TSLP antibody
Figure 1. Staining TSLP in Thymus by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue. Rabbit Aff - Purified Hu P, WB
50 µg / €425.00
  OriGene Technologies GmbH

TSLP antibody
Mouse Liver (formalin-fixed, paraffin-embedded) stained with TSLP antibody AP22809PU-N followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Purified Ms, Rt E, P, WB
50 µg / €425.00
  OriGene Technologies GmbH
+2 additional images

TSLP (C-term) antibody
TSLP Antibody (C-term) (AP54374PU-N) western blot analysis in Jurkat cell line lysates (35 µg/lane). This demonstrates the TSLP antibody detected the TSLP protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €415.00
  OriGene Technologies GmbH

TSLP antibody
TSLP Rat IgG2 5G29 Purified Ms WB
0.1 mg / €460.00
  OriGene Technologies GmbH

TSLP antibody
IHC staining of human tiisue frozen section with clone 214E4 (DX0480) Mouse IgG1 214E4.01 Purified Hu C
0.1 mg / €440.00
  Dendritics SAS

TSLP antibody
IHC staining of human tonsil frozen section with clone209A2 (DX0481) Mouse IgG2a 209A2.03 Purified Hu C, E, E(capture)
0.1 mg / €440.00
  Dendritics SAS
+1 additional image

TSLP antibody
TSLP Mouse IgG2a 210H1 Biotin Hu E, WB
0.1 mg / €490.00
  Dendritics SAS
+1 additional image

TSLP antibody
TSLP Mouse IgG2a 210H1 Purified Hu E, WB
0.1 mg / €440.00
  Dendritics SAS
+1 additional image

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

TSLP (transcript variant 1)
  Purified recombinant protein of Human thymic stromal lymphopoietin (TSLP), transcript variant 1.  
TSLP Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.		 E. coli
10 µg / €230.00
  OriGene Technologies, Inc.
 

TSLP (transcript variant 1)
  Purified recombinant protein of Human thymic stromal lymphopoietin (TSLP), transcript variant 1  
TSLP Human > 98 %
Preparation: .
Purity Detail: >98%, as determined by Coomassie stained SDS-PAGE.		 E. coli
10 µg / €255.00
  OriGene Technologies, Inc.
 

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