TTL antibody
Principal name
TTL antibody
Alternative names for TTL antibody
Tubulin-tyrosine ligase, Tubulin tyrosine ligase
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB)
Background of TTL antibody
TTL catalyzes the post-translatiol addition of a tyrosine to the C-termil end of detyrosited alpha-tubulin.TTL is a cytosolic enzyme involved in the posttranslatiol modification of alpha-tubulin (see MIM 602529). Alpha-tubulin within assembled microtubules is detyrosited over time at the C terminus. After microtubule disassembly, TTL restores the tyrosine residues and consequently participates in a cycle of tubulin detyrosition and tyrosition (Erck et al., 2003 [PubMed 14571137]).[supplied by OMIM].
Human Lung; WB Suggested Anti-TTL Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human Lung; TTL antibody - middle region (ARP53199_P050) in Human Lung cells using Western Blot
TTL Antibody (C-term) (AP54406PU-N) western blot analysis in Ramos cell line lysates (35 µg/lane). This demonstrates the TTL antibody detected the TTL protein (arrow).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TTL (RC207805, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TTL.
WB Suggested Anti-TTL Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Human Lung
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA502027 | TTL antibody |
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| TTL mouse monoclonal antibody, clone OTI3G5 (formerly 3G5) | |||||||
 |
Mouse | IgG1 | OTI3G5 | Purified | Hu | WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
|
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| AP54406PU-N | TTL (C-term) antibody |
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 |
Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP307805 | TTL |
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| Recombinant protein of human tubulin tyrosine ligase (TTL) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY403517 | TTL overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR205888L2V | Lenti ORF particles, Ttl (GFP-tagged) - Mouse tubulin tyrosine ligase (Ttl), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR205888L1V | Lenti ORF particles, Ttl (Myc-DDK-tagged) - Mouse tubulin tyrosine ligase (Ttl), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR213184L2V | Lenti ORF particles, Ttl (GFP-tagged ORF) - Rat tubulin tyrosine ligase (Ttl), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR213184L1V | Lenti ORF particles, Ttl (Myc-DDK-tagged ORF) - Rat tubulin tyrosine ligase (Ttl), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||