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TTL antibody

Principal name

TTL antibody

Alternative names for TTL antibody

Tubulin-tyrosine ligase, Tubulin tyrosine ligase

SwissProt ID

P38160 (Pig), P38584 (Bovin), P38585 (Mouse), Q8NG68 (Human), Q9QXJ0 (Rat)

Gene ID

150465 (TTL), 69737 (Ttl), 171572

Ncbi ID

9606, NP_714923, NP_714923.1, NM_153712, NM_027192, NM_138536

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Por (Porcine), Rt (Rat), Ze (Zebrafish), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB)

Background of TTL antibody

TTL catalyzes the post-translatiol addition of a tyrosine to the C-termil end of detyrosited alpha-tubulin.TTL is a cytosolic enzyme involved in the posttranslatiol modification of alpha-tubulin (see MIM 602529). Alpha-tubulin within assembled microtubules is detyrosited over time at the C terminus. After microtubule disassembly, TTL restores the tyrosine residues and consequently participates in a cycle of tubulin detyrosition and tyrosition (Erck et al., 2003 [PubMed 14571137]).[supplied by OMIM].

Human Lung; WB Suggested Anti-TTL Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human Lung; TTL antibody - middle region (ARP53199_P050) in Human Lung cells using Western Blot

ARP53199_P050

TTL Antibody (C-term) (AP54406PU-N) western blot analysis in Ramos cell line lysates (35 µg/lane). This demonstrates the TTL antibody detected the TTL protein (arrow).

AP54406PU-N

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TTL (RC207805, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TTL.